Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0177 and Shewana3_0178 are separated by 122 nucleotides Shewana3_0178 and Shewana3_0179 are separated by 69 nucleotides Shewana3_0179 and Shewana3_0180 are separated by 141 nucleotides Shewana3_0180 and Shewana3_0181 are separated by 11 nucleotides
Shewana3_0177: Shewana3_0177 - Delta-9 acyl-phospholipid desaturase (RefSeq), at 210,775 to 211,902
_0177
Shewana3_0178: Shewana3_0178 - DNA-binding transcriptional repressor FabR (RefSeq), at 212,025 to 212,639
_0178
Shewana3_0179: Shewana3_0179 - tRNA (uracil-5-)-methyltransferase (RefSeq), at 212,709 to 213,806
_0179
Shewana3_0180: Shewana3_0180 - glutamate racemase (RefSeq), at 213,948 to 214,769
_0180
Shewana3_0181: Shewana3_0181 - RNP-1-like RNA-binding protein (RefSeq), at 214,781 to 215,245
_0181
Position (kb) 212
213
214 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 211.712 kb on - strand, within Shewana3_0177 at 211.752 kb on + strand, within Shewana3_0177 at 211.815 kb on + strand at 211.820 kb on + strand at 211.851 kb on + strand at 211.859 kb on - strand at 212.040 kb on - strand at 212.090 kb on + strand, within Shewana3_0178 at 212.090 kb on + strand, within Shewana3_0178 at 212.090 kb on + strand, within Shewana3_0178 at 212.090 kb on + strand, within Shewana3_0178 at 212.123 kb on - strand, within Shewana3_0178 at 212.195 kb on + strand, within Shewana3_0178 at 212.230 kb on + strand, within Shewana3_0178 at 212.230 kb on + strand, within Shewana3_0178 at 212.230 kb on + strand, within Shewana3_0178 at 212.230 kb on + strand, within Shewana3_0178 at 212.238 kb on - strand, within Shewana3_0178 at 212.238 kb on - strand, within Shewana3_0178 at 212.238 kb on - strand, within Shewana3_0178 at 212.238 kb on - strand, within Shewana3_0178 at 212.238 kb on - strand, within Shewana3_0178 at 212.271 kb on + strand, within Shewana3_0178 at 212.391 kb on + strand, within Shewana3_0178 at 212.461 kb on + strand, within Shewana3_0178 at 212.461 kb on + strand, within Shewana3_0178 at 212.461 kb on + strand, within Shewana3_0178 at 212.469 kb on - strand, within Shewana3_0178 at 212.471 kb on + strand, within Shewana3_0178 at 212.491 kb on - strand, within Shewana3_0178 at 212.499 kb on + strand, within Shewana3_0178 at 212.600 kb on - strand at 212.618 kb on + strand at 212.734 kb on - strand at 212.757 kb on - strand at 212.757 kb on - strand at 212.806 kb on - strand at 212.875 kb on + strand, within Shewana3_0179 at 212.892 kb on + strand, within Shewana3_0179 at 212.943 kb on + strand, within Shewana3_0179 at 212.966 kb on + strand, within Shewana3_0179 at 213.100 kb on - strand, within Shewana3_0179 at 213.436 kb on - strand, within Shewana3_0179 at 213.563 kb on - strand, within Shewana3_0179 at 213.699 kb on + strand at 213.701 kb on + strand at 213.715 kb on + strand at 213.732 kb on + strand at 213.745 kb on - strand at 213.889 kb on + strand at 213.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 211,712 - Shewana3_0177 0.83 -0.3 211,752 + Shewana3_0177 0.87 -1.7 211,815 + +0.3 211,820 + +1.3 211,851 + +1.1 211,859 - +0.5 212,040 - +0.4 212,090 + Shewana3_0178 0.11 -1.4 212,090 + Shewana3_0178 0.11 +0.0 212,090 + Shewana3_0178 0.11 +0.2 212,090 + Shewana3_0178 0.11 +0.4 212,123 - Shewana3_0178 0.16 -1.3 212,195 + Shewana3_0178 0.28 +0.1 212,230 + Shewana3_0178 0.33 +0.7 212,230 + Shewana3_0178 0.33 +0.8 212,230 + Shewana3_0178 0.33 +0.3 212,230 + Shewana3_0178 0.33 +0.6 212,238 - Shewana3_0178 0.35 -1.4 212,238 - Shewana3_0178 0.35 +0.6 212,238 - Shewana3_0178 0.35 +0.3 212,238 - Shewana3_0178 0.35 -0.1 212,238 - Shewana3_0178 0.35 +0.2 212,271 + Shewana3_0178 0.40 +1.1 212,391 + Shewana3_0178 0.60 +0.3 212,461 + Shewana3_0178 0.71 +0.9 212,461 + Shewana3_0178 0.71 +0.6 212,461 + Shewana3_0178 0.71 -2.6 212,469 - Shewana3_0178 0.72 -1.7 212,471 + Shewana3_0178 0.73 -1.8 212,491 - Shewana3_0178 0.76 +1.1 212,499 + Shewana3_0178 0.77 -0.7 212,600 - +1.0 212,618 + -3.5 212,734 - +1.0 212,757 - -0.7 212,757 - +0.1 212,806 - -1.8 212,875 + Shewana3_0179 0.15 +1.4 212,892 + Shewana3_0179 0.17 +1.0 212,943 + Shewana3_0179 0.21 +0.4 212,966 + Shewana3_0179 0.23 -4.0 213,100 - Shewana3_0179 0.36 +1.3 213,436 - Shewana3_0179 0.66 -1.4 213,563 - Shewana3_0179 0.78 +0.7 213,699 + +1.0 213,701 + -0.5 213,715 + -2.0 213,732 + -1.5 213,745 - -0.8 213,889 + +0.6 213,889 + -0.7
Or see this region's nucleotide sequence