Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mogA and Shewana3_0071 are separated by 33 nucleotides Shewana3_0071 and Shewana3_0072 are separated by 151 nucleotides Shewana3_0072 and Shewana3_0073 are separated by 3 nucleotides Shewana3_0073 and Shewana3_0074 are separated by 49 nucleotides
Shewana3_0070: mogA - molybdenum cofactor biosynthesis protein (RefSeq), at 83,797 to 84,330
mogA
Shewana3_0071: Shewana3_0071 - hypothetical protein (RefSeq), at 84,364 to 84,582
_0071
Shewana3_0072: Shewana3_0072 - transcriptional regulator (RefSeq), at 84,734 to 85,777
_0072
Shewana3_0073: Shewana3_0073 - hypothetical protein (RefSeq), at 85,781 to 86,041
_0073
Shewana3_0074: Shewana3_0074 - transport protein, putative (RefSeq), at 86,091 to 87,230
_0074
Position (kb) 84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 83.775 kb on - strand at 83.807 kb on - strand at 83.984 kb on - strand, within mogA at 84.034 kb on - strand, within mogA at 84.055 kb on - strand, within mogA at 84.082 kb on + strand, within mogA at 84.254 kb on + strand, within mogA at 84.268 kb on - strand, within mogA at 84.272 kb on - strand, within mogA at 84.460 kb on - strand, within Shewana3_0071 at 84.505 kb on + strand, within Shewana3_0071 at 84.505 kb on + strand, within Shewana3_0071 at 84.513 kb on - strand, within Shewana3_0071 at 84.541 kb on + strand, within Shewana3_0071 at 84.542 kb on - strand, within Shewana3_0071 at 84.566 kb on + strand at 84.569 kb on - strand at 84.584 kb on - strand at 84.592 kb on - strand at 84.849 kb on + strand, within Shewana3_0072 at 84.849 kb on + strand, within Shewana3_0072 at 84.912 kb on + strand, within Shewana3_0072 at 85.071 kb on + strand, within Shewana3_0072 at 85.194 kb on + strand, within Shewana3_0072 at 85.227 kb on - strand, within Shewana3_0072 at 85.268 kb on - strand, within Shewana3_0072 at 85.272 kb on + strand, within Shewana3_0072 at 85.304 kb on + strand, within Shewana3_0072 at 85.380 kb on + strand, within Shewana3_0072 at 85.425 kb on - strand, within Shewana3_0072 at 85.517 kb on - strand, within Shewana3_0072 at 85.633 kb on - strand, within Shewana3_0072 at 85.689 kb on + strand at 85.710 kb on - strand at 85.769 kb on - strand at 86.004 kb on - strand, within Shewana3_0073 at 86.059 kb on - strand at 86.340 kb on + strand, within Shewana3_0074 at 86.348 kb on - strand, within Shewana3_0074 at 86.358 kb on - strand, within Shewana3_0074 at 86.359 kb on - strand, within Shewana3_0074 at 86.438 kb on - strand, within Shewana3_0074 at 86.469 kb on + strand, within Shewana3_0074 at 86.501 kb on - strand, within Shewana3_0074 at 86.501 kb on - strand, within Shewana3_0074 at 86.528 kb on - strand, within Shewana3_0074 at 86.530 kb on + strand, within Shewana3_0074 at 86.540 kb on + strand, within Shewana3_0074 at 86.646 kb on + strand, within Shewana3_0074 at 86.683 kb on + strand, within Shewana3_0074 at 86.733 kb on - strand, within Shewana3_0074
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 83,775 - +0.6 83,807 - +1.3 83,984 - mogA Shewana3_0070 0.35 -2.2 84,034 - mogA Shewana3_0070 0.44 -2.6 84,055 - mogA Shewana3_0070 0.48 +0.3 84,082 + mogA Shewana3_0070 0.53 -2.0 84,254 + mogA Shewana3_0070 0.86 +0.9 84,268 - mogA Shewana3_0070 0.88 +0.1 84,272 - mogA Shewana3_0070 0.89 +1.8 84,460 - Shewana3_0071 0.44 -1.9 84,505 + Shewana3_0071 0.64 -1.5 84,505 + Shewana3_0071 0.64 +0.4 84,513 - Shewana3_0071 0.68 -0.7 84,541 + Shewana3_0071 0.81 -2.3 84,542 - Shewana3_0071 0.81 -1.5 84,566 + -0.4 84,569 - -2.1 84,584 - +0.4 84,592 - +1.6 84,849 + Shewana3_0072 0.11 -0.7 84,849 + Shewana3_0072 0.11 +0.1 84,912 + Shewana3_0072 0.17 +1.1 85,071 + Shewana3_0072 0.32 +0.7 85,194 + Shewana3_0072 0.44 -1.2 85,227 - Shewana3_0072 0.47 +0.5 85,268 - Shewana3_0072 0.51 -1.3 85,272 + Shewana3_0072 0.52 -0.4 85,304 + Shewana3_0072 0.55 +0.4 85,380 + Shewana3_0072 0.62 +1.2 85,425 - Shewana3_0072 0.66 -1.5 85,517 - Shewana3_0072 0.75 +0.9 85,633 - Shewana3_0072 0.86 +0.6 85,689 + +0.5 85,710 - -1.6 85,769 - -0.0 86,004 - Shewana3_0073 0.85 -0.7 86,059 - +1.3 86,340 + Shewana3_0074 0.22 -0.2 86,348 - Shewana3_0074 0.23 +0.7 86,358 - Shewana3_0074 0.23 +0.4 86,359 - Shewana3_0074 0.24 +1.4 86,438 - Shewana3_0074 0.30 -3.1 86,469 + Shewana3_0074 0.33 +1.1 86,501 - Shewana3_0074 0.36 +0.8 86,501 - Shewana3_0074 0.36 -1.5 86,528 - Shewana3_0074 0.38 -2.3 86,530 + Shewana3_0074 0.39 -0.2 86,540 + Shewana3_0074 0.39 -3.5 86,646 + Shewana3_0074 0.49 +0.8 86,683 + Shewana3_0074 0.52 +0.1 86,733 - Shewana3_0074 0.56 +0.6
Or see this region's nucleotide sequence