Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4346 and Shewana3_4347 overlap by 4 nucleotides Shewana3_4347 and Shewana3_4348 are separated by 65 nucleotides Shewana3_4348 and Shewana3_4349 are separated by 178 nucleotides Shewana3_4349 and Shewana3_4350 are separated by 555 nucleotides
Shewana3_4346: Shewana3_4346 - transcriptional regulator MerD (RefSeq), at 232,097 to 232,462
_4346
Shewana3_4347: Shewana3_4347 - putative mercury resistance protein (RefSeq), at 232,459 to 232,695
_4347
Shewana3_4348: Shewana3_4348 - hypothetical protein (RefSeq), at 232,761 to 232,973
_4348
Shewana3_4349: Shewana3_4349 - umuC protein (RefSeq), at 233,152 to 233,817
_4349
Shewana3_4350: Shewana3_4350 - hypothetical protein (RefSeq), at 234,373 to 235,905
_4350
Position (kb) 233
234 Strain fitness (log2 ratio) -2
-1
0
1
2 at 232.439 kb on - strand at 232.464 kb on - strand at 232.616 kb on - strand, within Shewana3_4347 at 232.651 kb on - strand, within Shewana3_4347 at 232.732 kb on - strand at 232.910 kb on + strand, within Shewana3_4348 at 232.910 kb on + strand, within Shewana3_4348 at 232.910 kb on + strand, within Shewana3_4348 at 232.987 kb on - strand at 233.069 kb on - strand at 233.084 kb on - strand at 233.194 kb on + strand at 233.391 kb on + strand, within Shewana3_4349 at 233.563 kb on + strand, within Shewana3_4349 at 233.571 kb on - strand, within Shewana3_4349 at 233.571 kb on - strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.712 kb on + strand, within Shewana3_4349 at 233.720 kb on - strand, within Shewana3_4349 at 233.763 kb on + strand at 233.776 kb on - strand at 233.783 kb on - strand at 233.858 kb on - strand at 233.901 kb on - strand at 234.059 kb on + strand at 234.135 kb on + strand at 234.143 kb on - strand at 234.143 kb on - strand at 234.473 kb on + strand at 234.481 kb on - strand at 234.539 kb on + strand, within Shewana3_4350 at 234.547 kb on - strand, within Shewana3_4350 at 234.549 kb on + strand, within Shewana3_4350 at 234.557 kb on - strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.565 kb on + strand, within Shewana3_4350 at 234.573 kb on - strand, within Shewana3_4350 at 234.573 kb on - strand, within Shewana3_4350 at 234.573 kb on - strand, within Shewana3_4350 at 234.583 kb on - strand, within Shewana3_4350 at 234.583 kb on - strand, within Shewana3_4350 at 234.642 kb on + strand, within Shewana3_4350 at 234.652 kb on + strand, within Shewana3_4350 at 234.652 kb on + strand, within Shewana3_4350 at 234.661 kb on - strand, within Shewana3_4350 at 234.671 kb on - strand, within Shewana3_4350 at 234.761 kb on - strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.771 kb on + strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350 at 234.779 kb on - strand, within Shewana3_4350
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 232,439 - +0.6 232,464 - +1.0 232,616 - Shewana3_4347 0.66 -1.0 232,651 - Shewana3_4347 0.81 -1.3 232,732 - -1.6 232,910 + Shewana3_4348 0.70 +0.7 232,910 + Shewana3_4348 0.70 +0.5 232,910 + Shewana3_4348 0.70 +0.3 232,987 - +0.5 233,069 - +0.4 233,084 - -1.1 233,194 + +1.0 233,391 + Shewana3_4349 0.36 +0.8 233,563 + Shewana3_4349 0.62 +0.5 233,571 - Shewana3_4349 0.63 +0.3 233,571 - Shewana3_4349 0.63 -1.3 233,712 + Shewana3_4349 0.84 -1.0 233,712 + Shewana3_4349 0.84 -0.1 233,712 + Shewana3_4349 0.84 -0.0 233,712 + Shewana3_4349 0.84 +0.3 233,712 + Shewana3_4349 0.84 -0.2 233,712 + Shewana3_4349 0.84 +0.7 233,720 - Shewana3_4349 0.85 +0.4 233,763 + -0.1 233,776 - +0.3 233,783 - +0.7 233,858 - -0.0 233,901 - -0.6 234,059 + -1.1 234,135 + +1.4 234,143 - +0.2 234,143 - -0.7 234,473 + -1.9 234,481 - +0.6 234,539 + Shewana3_4350 0.11 +0.9 234,547 - Shewana3_4350 0.11 +0.3 234,549 + Shewana3_4350 0.11 +0.9 234,557 - Shewana3_4350 0.12 -0.9 234,565 + Shewana3_4350 0.13 +0.6 234,565 + Shewana3_4350 0.13 +1.1 234,565 + Shewana3_4350 0.13 +0.9 234,565 + Shewana3_4350 0.13 +0.8 234,573 - Shewana3_4350 0.13 -0.5 234,573 - Shewana3_4350 0.13 -0.4 234,573 - Shewana3_4350 0.13 -2.0 234,583 - Shewana3_4350 0.14 -1.0 234,583 - Shewana3_4350 0.14 -1.2 234,642 + Shewana3_4350 0.18 -1.7 234,652 + Shewana3_4350 0.18 -0.1 234,652 + Shewana3_4350 0.18 -0.3 234,661 - Shewana3_4350 0.19 +0.8 234,671 - Shewana3_4350 0.19 +0.9 234,761 - Shewana3_4350 0.25 +0.7 234,771 + Shewana3_4350 0.26 +0.5 234,771 + Shewana3_4350 0.26 -0.8 234,771 + Shewana3_4350 0.26 +0.8 234,771 + Shewana3_4350 0.26 +1.6 234,779 - Shewana3_4350 0.26 -1.7 234,779 - Shewana3_4350 0.26 -2.0 234,779 - Shewana3_4350 0.26 +0.2 234,779 - Shewana3_4350 0.26 +2.2
Or see this region's nucleotide sequence