Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3496 and Shew_3497 are separated by 239 nucleotides Shew_3497 and rpoZ are separated by 59 nucleotides rpoZ and Shew_3499 are separated by 13 nucleotides
Shew_3496: Shew_3496 - hypothetical protein (RefSeq), at 4,182,237 to 4,183,430
_3496
Shew_3497: Shew_3497 - guanylate kinase (RefSeq), at 4,183,670 to 4,184,293
_3497
Shew_3498: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 4,184,353 to 4,184,634
rpoZ
Shew_3499: Shew_3499 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,184,648 to 4,186,753
_3499
Position (kb) 4183
4184
4185 Strain fitness (log2 ratio) -1
0
1
2 at 4182.678 kb on - strand, within Shew_3496 at 4182.678 kb on - strand, within Shew_3496 at 4182.834 kb on + strand, within Shew_3496 at 4182.882 kb on - strand, within Shew_3496 at 4183.045 kb on - strand, within Shew_3496 at 4183.068 kb on - strand, within Shew_3496 at 4183.068 kb on - strand, within Shew_3496 at 4183.082 kb on + strand, within Shew_3496 at 4183.159 kb on + strand, within Shew_3496 at 4183.162 kb on - strand, within Shew_3496 at 4183.174 kb on + strand, within Shew_3496 at 4183.179 kb on + strand, within Shew_3496 at 4183.442 kb on + strand at 4183.519 kb on + strand at 4183.611 kb on + strand at 4184.446 kb on + strand, within rpoZ at 4184.556 kb on + strand, within rpoZ
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 4,182,678 - Shew_3496 0.37 +0.4 4,182,678 - Shew_3496 0.37 +1.0 4,182,834 + Shew_3496 0.50 +0.7 4,182,882 - Shew_3496 0.54 -0.7 4,183,045 - Shew_3496 0.68 +0.4 4,183,068 - Shew_3496 0.70 +0.2 4,183,068 - Shew_3496 0.70 +0.2 4,183,082 + Shew_3496 0.71 -0.6 4,183,159 + Shew_3496 0.77 +0.4 4,183,162 - Shew_3496 0.77 +0.2 4,183,174 + Shew_3496 0.78 +0.2 4,183,179 + Shew_3496 0.79 +0.1 4,183,442 + +1.8 4,183,519 + +0.9 4,183,611 + +1.6 4,184,446 + rpoZ Shew_3498 0.33 +2.4 4,184,556 + rpoZ Shew_3498 0.72 +0.7
Or see this region's nucleotide sequence