Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2887 and Shew_2888 are separated by 63 nucleotides Shew_2888 and Shew_2889 are separated by 201 nucleotides Shew_2889 and Shew_2890 are separated by 19 nucleotides
Shew_2887: Shew_2887 - sterol desaturase family protein (RefSeq), at 3,426,234 to 3,427,148
_2887
Shew_2888: Shew_2888 - hypothetical protein (RefSeq), at 3,427,212 to 3,428,264
_2888
Shew_2889: Shew_2889 - uncharacterized lipoprotein (RefSeq), at 3,428,466 to 3,429,032
_2889
Shew_2890: Shew_2890 - peptidyl-prolyl cis-trans isomerase, cyclophilin type (RefSeq), at 3,429,052 to 3,429,636
_2890
Position (kb)
3427
3428
3429 Strain fitness (log2 ratio)
-2
-1
0
1 at 3426.245 kb on + strand at 3426.253 kb on - strand at 3426.495 kb on + strand, within Shew_2887 at 3426.516 kb on - strand, within Shew_2887 at 3427.280 kb on - strand at 3427.280 kb on - strand at 3427.305 kb on + strand at 3427.544 kb on + strand, within Shew_2888 at 3427.777 kb on - strand, within Shew_2888 at 3427.801 kb on + strand, within Shew_2888 at 3428.154 kb on + strand, within Shew_2888 at 3428.237 kb on - strand at 3428.521 kb on + strand at 3428.579 kb on + strand, within Shew_2889 at 3428.677 kb on + strand, within Shew_2889 at 3428.688 kb on - strand, within Shew_2889 at 3428.899 kb on - strand, within Shew_2889
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 3,426,245 + -0.2 3,426,253 - +0.2 3,426,495 + Shew_2887 0.29 +0.0 3,426,516 - Shew_2887 0.31 -0.2 3,427,280 - +0.6 3,427,280 - +0.4 3,427,305 + -0.2 3,427,544 + Shew_2888 0.32 +0.1 3,427,777 - Shew_2888 0.54 +0.2 3,427,801 + Shew_2888 0.56 -0.1 3,428,154 + Shew_2888 0.89 -0.8 3,428,237 - -0.0 3,428,521 + +0.2 3,428,579 + Shew_2889 0.20 +0.1 3,428,677 + Shew_2889 0.37 -0.7 3,428,688 - Shew_2889 0.39 -2.4 3,428,899 - Shew_2889 0.76 -2.4
Or see this region's nucleotide sequence