Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2643 and Shew_2644 are separated by 10 nucleotides Shew_2644 and Shew_2645 are separated by 183 nucleotides Shew_2645 and Shew_2646 overlap by 4 nucleotides
Shew_2643: Shew_2643 - pseudouridylate synthase (RefSeq), at 3,118,781 to 3,119,602
_2643
Shew_2644: Shew_2644 - hypothetical protein (RefSeq), at 3,119,613 to 3,119,930
_2644
Shew_2645: Shew_2645 - hypothetical protein (RefSeq), at 3,120,114 to 3,121,127
_2645
Shew_2646: Shew_2646 - hypothetical protein (RefSeq), at 3,121,124 to 3,121,513
_2646
Position (kb)
3120
3121
3122 Strain fitness (log2 ratio)
-1
0
1 at 3119.245 kb on + strand, within Shew_2643 at 3119.717 kb on - strand, within Shew_2644 at 3119.814 kb on + strand, within Shew_2644 at 3120.009 kb on + strand at 3120.224 kb on + strand, within Shew_2645 at 3120.250 kb on - strand, within Shew_2645 at 3120.451 kb on - strand, within Shew_2645 at 3120.508 kb on - strand, within Shew_2645 at 3120.584 kb on - strand, within Shew_2645 at 3120.589 kb on - strand, within Shew_2645 at 3120.598 kb on + strand, within Shew_2645 at 3120.961 kb on - strand, within Shew_2645 at 3121.083 kb on - strand at 3121.106 kb on + strand at 3121.286 kb on - strand, within Shew_2646 at 3121.970 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 3,119,245 + Shew_2643 0.56 +1.1 3,119,717 - Shew_2644 0.33 +1.0 3,119,814 + Shew_2644 0.63 +1.2 3,120,009 + -0.2 3,120,224 + Shew_2645 0.11 +0.6 3,120,250 - Shew_2645 0.13 -0.7 3,120,451 - Shew_2645 0.33 +0.5 3,120,508 - Shew_2645 0.39 -0.6 3,120,584 - Shew_2645 0.46 -1.2 3,120,589 - Shew_2645 0.47 -0.8 3,120,598 + Shew_2645 0.48 -0.3 3,120,961 - Shew_2645 0.84 +0.1 3,121,083 - -0.4 3,121,106 + +0.6 3,121,286 - Shew_2646 0.42 +0.8 3,121,970 - -0.1
Or see this region's nucleotide sequence