Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1048 and Shew_1049 are separated by 39 nucleotides Shew_1049 and Shew_1050 are separated by 65 nucleotides Shew_1050 and Shew_1051 are separated by 7 nucleotides Shew_1051 and Shew_1052 are separated by 108 nucleotides
Shew_1048: Shew_1048 - RNA polymerase sigma factor RpoE (RefSeq), at 1,211,260 to 1,211,838
_1048
Shew_1049: Shew_1049 - anti sigma-E protein, RseA (RefSeq), at 1,211,878 to 1,212,480
_1049
Shew_1050: Shew_1050 - sigma E regulatory protein, MucB/RseB (RefSeq), at 1,212,546 to 1,213,478
_1050
Shew_1051: Shew_1051 - positive regulator of sigma E, RseC/MucC (RefSeq), at 1,213,486 to 1,213,962
_1051
Shew_1052: Shew_1052 - GTP-binding protein LepA (RefSeq), at 1,214,071 to 1,215,861
_1052
Position (kb)
1212
1213
1214 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1211.746 kb on + strand, within Shew_1048 at 1211.791 kb on - strand at 1212.453 kb on + strand at 1212.453 kb on + strand at 1212.758 kb on + strand, within Shew_1050 at 1212.758 kb on + strand, within Shew_1050 at 1212.758 kb on + strand, within Shew_1050 at 1212.852 kb on + strand, within Shew_1050 at 1214.271 kb on - strand, within Shew_1052 at 1214.390 kb on + strand, within Shew_1052
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 1,211,746 + Shew_1048 0.84 -0.1 1,211,791 - -0.4 1,212,453 + +0.5 1,212,453 + +1.2 1,212,758 + Shew_1050 0.23 -1.7 1,212,758 + Shew_1050 0.23 -2.3 1,212,758 + Shew_1050 0.23 -3.0 1,212,852 + Shew_1050 0.33 -4.7 1,214,271 - Shew_1052 0.11 +1.4 1,214,390 + Shew_1052 0.18 +0.2
Or see this region's nucleotide sequence