Experiment: Paraquat dichloride 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0405 and Shew_0406 overlap by 1 nucleotides Shew_0406 and Shew_0407 are separated by 97 nucleotides Shew_0407 and Shew_0408 overlap by 1 nucleotides
Shew_0405: Shew_0405 - rod shape-determining protein MreD (RefSeq), at 455,056 to 455,544
_0405
Shew_0406: Shew_0406 - maf protein (RefSeq), at 455,544 to 456,134
_0406
Shew_0407: Shew_0407 - ribonuclease G (RefSeq), at 456,232 to 457,734
_0407
Shew_0408: Shew_0408 - hypothetical protein (RefSeq), at 457,734 to 462,044
_0408
Position (kb) 456
457
458 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 455.556 kb on + strand at 455.714 kb on + strand, within Shew_0406 at 455.796 kb on + strand, within Shew_0406 at 455.865 kb on + strand, within Shew_0406 at 455.969 kb on - strand, within Shew_0406 at 456.345 kb on + strand at 456.631 kb on + strand, within Shew_0407 at 456.840 kb on + strand, within Shew_0407 at 457.184 kb on - strand, within Shew_0407 at 457.203 kb on + strand, within Shew_0407 at 457.219 kb on + strand, within Shew_0407 at 457.834 kb on - strand at 457.834 kb on - strand at 458.047 kb on + strand at 458.232 kb on + strand, within Shew_0408 at 458.427 kb on + strand, within Shew_0408 at 458.427 kb on + strand, within Shew_0408 at 458.548 kb on - strand, within Shew_0408 at 458.732 kb on - strand, within Shew_0408
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 10 mg/ml remove 455,556 + +0.2 455,714 + Shew_0406 0.29 -0.1 455,796 + Shew_0406 0.43 -0.0 455,865 + Shew_0406 0.54 -1.0 455,969 - Shew_0406 0.72 -1.2 456,345 + -1.2 456,631 + Shew_0407 0.27 -1.5 456,840 + Shew_0407 0.40 -1.6 457,184 - Shew_0407 0.63 -3.5 457,203 + Shew_0407 0.65 -3.0 457,219 + Shew_0407 0.66 -4.3 457,834 - -0.1 457,834 - -4.7 458,047 + +0.4 458,232 + Shew_0408 0.12 +1.1 458,427 + Shew_0408 0.16 -0.5 458,427 + Shew_0408 0.16 +0.4 458,548 - Shew_0408 0.19 +0.9 458,732 - Shew_0408 0.23 +0.2
Or see this region's nucleotide sequence