Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5835 and Pf6N2E2_5836 overlap by 11 nucleotides Pf6N2E2_5836 and Pf6N2E2_5837 are separated by 88 nucleotides
Pf6N2E2_5835 - Pyruvate kinase (EC 2.7.1.40), at 6,651,588 to 6,653,003
_5835
Pf6N2E2_5836 - D-glycerate 2-kinase (EC 2.7.1.-), at 6,652,993 to 6,654,273
_5836
Pf6N2E2_5837 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 6,654,362 to 6,655,252
_5837
Position (kb)
6652
6653
6654
6655 Strain fitness (log2 ratio)
-2
-1
0
1 at 6652.923 kb on + strand at 6652.924 kb on - strand at 6653.096 kb on + strand at 6653.096 kb on + strand at 6653.237 kb on + strand, within Pf6N2E2_5836 at 6653.237 kb on + strand, within Pf6N2E2_5836 at 6653.237 kb on + strand, within Pf6N2E2_5836 at 6653.237 kb on + strand, within Pf6N2E2_5836 at 6653.237 kb on + strand, within Pf6N2E2_5836 at 6653.238 kb on - strand, within Pf6N2E2_5836 at 6653.238 kb on - strand, within Pf6N2E2_5836 at 6653.733 kb on - strand, within Pf6N2E2_5836 at 6653.733 kb on - strand, within Pf6N2E2_5836 at 6653.733 kb on - strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.738 kb on + strand, within Pf6N2E2_5836 at 6653.739 kb on - strand, within Pf6N2E2_5836 at 6653.739 kb on - strand, within Pf6N2E2_5836 at 6653.739 kb on - strand, within Pf6N2E2_5836 at 6653.739 kb on - strand, within Pf6N2E2_5836 at 6653.739 kb on - strand, within Pf6N2E2_5836 at 6653.740 kb on + strand, within Pf6N2E2_5836 at 6654.188 kb on + strand at 6654.188 kb on + strand at 6654.188 kb on + strand at 6654.188 kb on + strand at 6654.188 kb on + strand at 6654.188 kb on + strand at 6654.189 kb on - strand at 6654.189 kb on - strand at 6654.292 kb on - strand at 6654.295 kb on - strand at 6654.363 kb on + strand at 6654.363 kb on + strand at 6654.363 kb on + strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.364 kb on - strand at 6654.392 kb on - strand at 6654.392 kb on - strand at 6654.481 kb on + strand, within Pf6N2E2_5837 at 6655.272 kb on + strand at 6655.272 kb on + strand at 6655.272 kb on + strand at 6655.272 kb on + strand at 6655.273 kb on - strand at 6655.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 6,652,923 + +1.5 6,652,924 - +1.3 6,653,096 + +0.2 6,653,096 + -0.4 6,653,237 + Pf6N2E2_5836 0.19 -0.1 6,653,237 + Pf6N2E2_5836 0.19 -0.0 6,653,237 + Pf6N2E2_5836 0.19 +0.6 6,653,237 + Pf6N2E2_5836 0.19 -0.8 6,653,237 + Pf6N2E2_5836 0.19 +0.1 6,653,238 - Pf6N2E2_5836 0.19 +0.5 6,653,238 - Pf6N2E2_5836 0.19 +1.1 6,653,733 - Pf6N2E2_5836 0.58 -0.3 6,653,733 - Pf6N2E2_5836 0.58 -0.2 6,653,733 - Pf6N2E2_5836 0.58 -0.5 6,653,738 + Pf6N2E2_5836 0.58 -1.1 6,653,738 + Pf6N2E2_5836 0.58 -0.1 6,653,738 + Pf6N2E2_5836 0.58 -0.4 6,653,738 + Pf6N2E2_5836 0.58 -0.9 6,653,738 + Pf6N2E2_5836 0.58 -1.8 6,653,738 + Pf6N2E2_5836 0.58 +0.3 6,653,738 + Pf6N2E2_5836 0.58 -0.2 6,653,739 - Pf6N2E2_5836 0.58 +0.3 6,653,739 - Pf6N2E2_5836 0.58 -0.1 6,653,739 - Pf6N2E2_5836 0.58 +0.5 6,653,739 - Pf6N2E2_5836 0.58 -1.4 6,653,739 - Pf6N2E2_5836 0.58 +0.4 6,653,740 + Pf6N2E2_5836 0.58 -0.6 6,654,188 + -1.7 6,654,188 + +0.2 6,654,188 + -0.5 6,654,188 + +0.9 6,654,188 + -0.1 6,654,188 + -0.7 6,654,189 - -0.0 6,654,189 - +0.9 6,654,292 - -1.1 6,654,295 - -0.8 6,654,363 + -0.7 6,654,363 + +0.1 6,654,363 + +1.1 6,654,364 - +0.1 6,654,364 - +1.3 6,654,364 - +0.4 6,654,364 - +0.1 6,654,364 - -2.1 6,654,364 - +0.2 6,654,364 - -0.6 6,654,364 - +0.8 6,654,392 - +0.5 6,654,392 - -0.2 6,654,481 + Pf6N2E2_5837 0.13 -0.9 6,655,272 + -1.3 6,655,272 + +0.4 6,655,272 + -0.7 6,655,272 + +0.1 6,655,273 - +0.7 6,655,273 - +0.1
Or see this region's nucleotide sequence