Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5592 and Pf6N2E2_5593 are separated by 55 nucleotides Pf6N2E2_5593 and Pf6N2E2_5594 are separated by 107 nucleotides Pf6N2E2_5594 and Pf6N2E2_5595 overlap by 4 nucleotides
Pf6N2E2_5592 - DNA-binding response regulator, LuxR family, at 6,409,737 to 6,410,363
_5592
Pf6N2E2_5593 - Leucyl-tRNA synthetase, at 6,410,419 to 6,410,601
_5593
Pf6N2E2_5594 - Endonuclease III (EC 4.2.99.18), at 6,410,709 to 6,411,347
_5594
Pf6N2E2_5595 - Electron transport complex protein RnfE, at 6,411,344 to 6,411,832
_5595
Position (kb)
6410
6411 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6409.491 kb on + strand at 6409.619 kb on + strand at 6409.619 kb on + strand at 6409.626 kb on + strand at 6409.627 kb on - strand at 6409.677 kb on - strand at 6409.691 kb on + strand at 6409.786 kb on + strand at 6409.812 kb on + strand, within Pf6N2E2_5592 at 6409.871 kb on + strand, within Pf6N2E2_5592 at 6410.034 kb on + strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.035 kb on - strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.038 kb on + strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.039 kb on - strand, within Pf6N2E2_5592 at 6410.057 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.068 kb on + strand, within Pf6N2E2_5592 at 6410.069 kb on - strand, within Pf6N2E2_5592 at 6410.126 kb on + strand, within Pf6N2E2_5592 at 6410.276 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.280 kb on + strand, within Pf6N2E2_5592 at 6410.281 kb on - strand, within Pf6N2E2_5592 at 6410.380 kb on + strand at 6410.380 kb on + strand at 6410.387 kb on + strand at 6410.413 kb on + strand at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.572 kb on + strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.573 kb on - strand, within Pf6N2E2_5593 at 6410.618 kb on + strand at 6410.619 kb on - strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.640 kb on + strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.641 kb on - strand at 6410.735 kb on - strand at 6410.742 kb on + strand at 6410.743 kb on - strand at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.124 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.154 kb on + strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.155 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.171 kb on - strand, within Pf6N2E2_5594 at 6411.272 kb on - strand, within Pf6N2E2_5594 at 6411.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 6,409,491 + -2.5 6,409,619 + -2.3 6,409,619 + +0.2 6,409,626 + -3.1 6,409,627 - -1.1 6,409,677 - -3.3 6,409,691 + -4.0 6,409,786 + +0.6 6,409,812 + Pf6N2E2_5592 0.12 +0.9 6,409,871 + Pf6N2E2_5592 0.21 -0.6 6,410,034 + Pf6N2E2_5592 0.47 -0.0 6,410,035 - Pf6N2E2_5592 0.48 -0.1 6,410,035 - Pf6N2E2_5592 0.48 -0.8 6,410,035 - Pf6N2E2_5592 0.48 +2.0 6,410,038 + Pf6N2E2_5592 0.48 -0.2 6,410,038 + Pf6N2E2_5592 0.48 +0.0 6,410,038 + Pf6N2E2_5592 0.48 +0.7 6,410,038 + Pf6N2E2_5592 0.48 +1.0 6,410,038 + Pf6N2E2_5592 0.48 +0.1 6,410,038 + Pf6N2E2_5592 0.48 -1.4 6,410,038 + Pf6N2E2_5592 0.48 +0.7 6,410,039 - Pf6N2E2_5592 0.48 +0.6 6,410,039 - Pf6N2E2_5592 0.48 -0.2 6,410,039 - Pf6N2E2_5592 0.48 +0.8 6,410,039 - Pf6N2E2_5592 0.48 -0.5 6,410,039 - Pf6N2E2_5592 0.48 +0.7 6,410,057 + Pf6N2E2_5592 0.51 -0.3 6,410,068 + Pf6N2E2_5592 0.53 +0.1 6,410,068 + Pf6N2E2_5592 0.53 +0.0 6,410,068 + Pf6N2E2_5592 0.53 -0.6 6,410,069 - Pf6N2E2_5592 0.53 +0.5 6,410,126 + Pf6N2E2_5592 0.62 -0.2 6,410,276 + Pf6N2E2_5592 0.86 -0.0 6,410,280 + Pf6N2E2_5592 0.87 -0.0 6,410,280 + Pf6N2E2_5592 0.87 +0.1 6,410,280 + Pf6N2E2_5592 0.87 +1.6 6,410,281 - Pf6N2E2_5592 0.87 -0.3 6,410,380 + +1.4 6,410,380 + -0.6 6,410,387 + +0.5 6,410,413 + +0.9 6,410,572 + Pf6N2E2_5593 0.84 -1.0 6,410,572 + Pf6N2E2_5593 0.84 -1.2 6,410,572 + Pf6N2E2_5593 0.84 +0.7 6,410,573 - Pf6N2E2_5593 0.84 -0.4 6,410,573 - Pf6N2E2_5593 0.84 +0.8 6,410,573 - Pf6N2E2_5593 0.84 +0.0 6,410,573 - Pf6N2E2_5593 0.84 -1.6 6,410,573 - Pf6N2E2_5593 0.84 -1.3 6,410,618 + -1.0 6,410,619 - -0.7 6,410,640 + -1.1 6,410,640 + +0.4 6,410,640 + -1.8 6,410,640 + -1.1 6,410,641 - -1.4 6,410,641 - -1.6 6,410,641 - -0.7 6,410,641 - -0.8 6,410,641 - -1.0 6,410,641 - -0.3 6,410,641 - -0.4 6,410,641 - -0.3 6,410,641 - -0.4 6,410,735 - +1.4 6,410,742 + -0.5 6,410,743 - -0.9 6,411,124 + Pf6N2E2_5594 0.65 +0.2 6,411,124 + Pf6N2E2_5594 0.65 -0.1 6,411,124 + Pf6N2E2_5594 0.65 -0.2 6,411,154 + Pf6N2E2_5594 0.70 +0.1 6,411,154 + Pf6N2E2_5594 0.70 +0.8 6,411,154 + Pf6N2E2_5594 0.70 +0.9 6,411,154 + Pf6N2E2_5594 0.70 -0.6 6,411,155 - Pf6N2E2_5594 0.70 -0.4 6,411,155 - Pf6N2E2_5594 0.70 -1.7 6,411,171 - Pf6N2E2_5594 0.72 -0.6 6,411,171 - Pf6N2E2_5594 0.72 +0.8 6,411,171 - Pf6N2E2_5594 0.72 -0.0 6,411,272 - Pf6N2E2_5594 0.88 -1.3 6,411,321 + +0.1
Or see this region's nucleotide sequence