Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5491 and Pf6N2E2_5492 overlap by 8 nucleotides Pf6N2E2_5492 and Pf6N2E2_5493 are separated by 28 nucleotides Pf6N2E2_5493 and Pf6N2E2_5494 are separated by 101 nucleotides
Pf6N2E2_5491 - FOG: GGDEF domain, at 6,309,738 to 6,310,844
_5491
Pf6N2E2_5492 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 6,310,837 to 6,311,772
_5492
Pf6N2E2_5493 - Enoyl-CoA hydratase (EC 4.2.1.17), at 6,311,801 to 6,312,550
_5493
Pf6N2E2_5494 - Pyruvate kinase (EC 2.7.1.40), at 6,312,652 to 6,314,103
_5494
Position (kb)
6311
6312
6313 Strain fitness (log2 ratio)
-1
0
1
2 at 6310.888 kb on - strand at 6310.930 kb on + strand at 6310.930 kb on + strand at 6311.389 kb on + strand, within Pf6N2E2_5492 at 6311.389 kb on + strand, within Pf6N2E2_5492 at 6311.389 kb on + strand, within Pf6N2E2_5492 at 6311.390 kb on - strand, within Pf6N2E2_5492 at 6311.390 kb on - strand, within Pf6N2E2_5492 at 6311.509 kb on - strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.666 kb on + strand, within Pf6N2E2_5492 at 6311.667 kb on - strand, within Pf6N2E2_5492 at 6311.713 kb on + strand at 6311.713 kb on + strand at 6311.714 kb on - strand at 6311.714 kb on - strand at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.894 kb on + strand, within Pf6N2E2_5493 at 6311.895 kb on - strand, within Pf6N2E2_5493 at 6311.895 kb on - strand, within Pf6N2E2_5493 at 6311.895 kb on - strand, within Pf6N2E2_5493 at 6311.895 kb on - strand, within Pf6N2E2_5493 at 6311.895 kb on - strand, within Pf6N2E2_5493 at 6311.895 kb on - strand, within Pf6N2E2_5493 at 6312.152 kb on + strand, within Pf6N2E2_5493 at 6312.152 kb on + strand, within Pf6N2E2_5493 at 6312.152 kb on + strand, within Pf6N2E2_5493 at 6312.152 kb on + strand, within Pf6N2E2_5493 at 6312.153 kb on - strand, within Pf6N2E2_5493 at 6312.153 kb on - strand, within Pf6N2E2_5493 at 6312.412 kb on + strand, within Pf6N2E2_5493 at 6312.413 kb on - strand, within Pf6N2E2_5493 at 6312.413 kb on - strand, within Pf6N2E2_5493 at 6312.535 kb on + strand at 6312.548 kb on + strand at 6312.548 kb on + strand at 6312.548 kb on + strand at 6312.549 kb on - strand at 6312.549 kb on - strand at 6312.576 kb on + strand at 6312.576 kb on + strand at 6312.576 kb on + strand at 6312.576 kb on + strand at 6312.713 kb on + strand at 6312.713 kb on + strand at 6312.714 kb on - strand at 6312.896 kb on + strand, within Pf6N2E2_5494 at 6312.896 kb on + strand, within Pf6N2E2_5494 at 6312.897 kb on - strand, within Pf6N2E2_5494 at 6312.897 kb on - strand, within Pf6N2E2_5494 at 6312.998 kb on + strand, within Pf6N2E2_5494 at 6312.998 kb on + strand, within Pf6N2E2_5494 at 6312.999 kb on - strand, within Pf6N2E2_5494 at 6312.999 kb on - strand, within Pf6N2E2_5494 at 6313.178 kb on + strand, within Pf6N2E2_5494 at 6313.198 kb on + strand, within Pf6N2E2_5494 at 6313.494 kb on - strand, within Pf6N2E2_5494 at 6313.536 kb on - strand, within Pf6N2E2_5494
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 6,310,888 - -1.4 6,310,930 + -0.8 6,310,930 + -0.1 6,311,389 + Pf6N2E2_5492 0.59 +0.1 6,311,389 + Pf6N2E2_5492 0.59 -0.1 6,311,389 + Pf6N2E2_5492 0.59 +1.2 6,311,390 - Pf6N2E2_5492 0.59 -1.0 6,311,390 - Pf6N2E2_5492 0.59 +0.7 6,311,509 - Pf6N2E2_5492 0.72 -1.3 6,311,666 + Pf6N2E2_5492 0.89 -0.3 6,311,666 + Pf6N2E2_5492 0.89 -1.0 6,311,666 + Pf6N2E2_5492 0.89 -0.2 6,311,666 + Pf6N2E2_5492 0.89 -1.3 6,311,667 - Pf6N2E2_5492 0.89 -0.1 6,311,713 + -0.4 6,311,713 + -1.5 6,311,714 - -0.2 6,311,714 - +0.1 6,311,894 + Pf6N2E2_5493 0.12 +0.5 6,311,894 + Pf6N2E2_5493 0.12 +0.3 6,311,894 + Pf6N2E2_5493 0.12 -0.0 6,311,894 + Pf6N2E2_5493 0.12 +0.7 6,311,894 + Pf6N2E2_5493 0.12 +1.3 6,311,894 + Pf6N2E2_5493 0.12 -0.2 6,311,894 + Pf6N2E2_5493 0.12 -0.7 6,311,894 + Pf6N2E2_5493 0.12 -0.8 6,311,894 + Pf6N2E2_5493 0.12 -0.3 6,311,895 - Pf6N2E2_5493 0.13 +0.7 6,311,895 - Pf6N2E2_5493 0.13 -0.2 6,311,895 - Pf6N2E2_5493 0.13 +0.1 6,311,895 - Pf6N2E2_5493 0.13 +0.2 6,311,895 - Pf6N2E2_5493 0.13 +0.3 6,311,895 - Pf6N2E2_5493 0.13 -0.4 6,312,152 + Pf6N2E2_5493 0.47 -0.4 6,312,152 + Pf6N2E2_5493 0.47 -0.2 6,312,152 + Pf6N2E2_5493 0.47 -1.7 6,312,152 + Pf6N2E2_5493 0.47 -0.1 6,312,153 - Pf6N2E2_5493 0.47 -0.6 6,312,153 - Pf6N2E2_5493 0.47 +1.0 6,312,412 + Pf6N2E2_5493 0.81 -0.2 6,312,413 - Pf6N2E2_5493 0.82 +0.8 6,312,413 - Pf6N2E2_5493 0.82 +0.3 6,312,535 + +0.3 6,312,548 + -0.0 6,312,548 + -0.2 6,312,548 + +0.4 6,312,549 - +0.3 6,312,549 - +1.0 6,312,576 + +0.8 6,312,576 + +0.1 6,312,576 + +0.1 6,312,576 + +0.3 6,312,713 + +0.3 6,312,713 + +1.6 6,312,714 - +0.4 6,312,896 + Pf6N2E2_5494 0.17 +0.1 6,312,896 + Pf6N2E2_5494 0.17 +2.1 6,312,897 - Pf6N2E2_5494 0.17 +0.8 6,312,897 - Pf6N2E2_5494 0.17 -0.1 6,312,998 + Pf6N2E2_5494 0.24 +1.6 6,312,998 + Pf6N2E2_5494 0.24 +0.7 6,312,999 - Pf6N2E2_5494 0.24 +0.3 6,312,999 - Pf6N2E2_5494 0.24 +0.4 6,313,178 + Pf6N2E2_5494 0.36 +1.1 6,313,198 + Pf6N2E2_5494 0.38 +0.2 6,313,494 - Pf6N2E2_5494 0.58 -0.3 6,313,536 - Pf6N2E2_5494 0.61 +1.4
Or see this region's nucleotide sequence