Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5467 and Pf6N2E2_5468 are separated by 271 nucleotides Pf6N2E2_5468 and Pf6N2E2_5469 are separated by 24 nucleotides Pf6N2E2_5469 and Pf6N2E2_5470 overlap by 4 nucleotides Pf6N2E2_5470 and Pf6N2E2_5471 are separated by 466 nucleotides
Pf6N2E2_5467 - Response regulator, at 6,282,164 to 6,283,531
_5467
Pf6N2E2_5468 - Nudix hydrolase family protein PA3470, at 6,283,803 to 6,284,219
_5468
Pf6N2E2_5469 - L-lysine permease, at 6,284,244 to 6,284,882
_5469
Pf6N2E2_5470 - acetyltransferase, GNAT family, at 6,284,879 to 6,285,376
_5470
Pf6N2E2_5471 - Transcriptional activator MetR, at 6,285,843 to 6,286,760
_5471
Position (kb)
6284
6285 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6283.275 kb on - strand, within Pf6N2E2_5467 at 6283.275 kb on - strand, within Pf6N2E2_5467 at 6283.685 kb on + strand at 6283.685 kb on + strand at 6283.685 kb on + strand at 6283.685 kb on + strand at 6283.686 kb on - strand at 6283.691 kb on + strand at 6283.692 kb on - strand at 6283.692 kb on - strand at 6283.763 kb on - strand at 6283.763 kb on - strand at 6283.976 kb on - strand, within Pf6N2E2_5468 at 6284.046 kb on - strand, within Pf6N2E2_5468 at 6284.062 kb on + strand, within Pf6N2E2_5468 at 6284.191 kb on - strand at 6284.206 kb on + strand at 6284.206 kb on + strand at 6284.206 kb on + strand at 6284.207 kb on - strand at 6284.207 kb on - strand at 6284.207 kb on - strand at 6284.207 kb on - strand at 6284.207 kb on - strand at 6284.210 kb on + strand at 6284.211 kb on - strand at 6284.211 kb on - strand at 6284.211 kb on - strand at 6284.211 kb on - strand at 6284.297 kb on - strand at 6284.297 kb on - strand at 6284.322 kb on + strand, within Pf6N2E2_5469 at 6284.322 kb on + strand, within Pf6N2E2_5469 at 6284.322 kb on + strand, within Pf6N2E2_5469 at 6284.322 kb on + strand, within Pf6N2E2_5469 at 6284.322 kb on + strand, within Pf6N2E2_5469 at 6284.322 kb on + strand, within Pf6N2E2_5469 at 6284.323 kb on - strand, within Pf6N2E2_5469 at 6284.323 kb on - strand, within Pf6N2E2_5469 at 6284.323 kb on - strand, within Pf6N2E2_5469 at 6284.323 kb on - strand, within Pf6N2E2_5469 at 6284.461 kb on - strand, within Pf6N2E2_5469 at 6284.467 kb on + strand, within Pf6N2E2_5469 at 6284.467 kb on + strand, within Pf6N2E2_5469 at 6284.634 kb on + strand, within Pf6N2E2_5469 at 6284.634 kb on + strand, within Pf6N2E2_5469 at 6284.634 kb on + strand, within Pf6N2E2_5469 at 6284.634 kb on + strand, within Pf6N2E2_5469 at 6284.634 kb on + strand, within Pf6N2E2_5469 at 6284.635 kb on - strand, within Pf6N2E2_5469 at 6284.697 kb on - strand, within Pf6N2E2_5469 at 6284.918 kb on + strand at 6284.942 kb on + strand, within Pf6N2E2_5470 at 6284.942 kb on + strand, within Pf6N2E2_5470 at 6284.942 kb on + strand, within Pf6N2E2_5470 at 6285.039 kb on + strand, within Pf6N2E2_5470 at 6285.039 kb on + strand, within Pf6N2E2_5470 at 6285.039 kb on + strand, within Pf6N2E2_5470 at 6285.040 kb on - strand, within Pf6N2E2_5470 at 6285.040 kb on - strand, within Pf6N2E2_5470 at 6285.040 kb on - strand, within Pf6N2E2_5470 at 6285.040 kb on - strand, within Pf6N2E2_5470 at 6285.040 kb on - strand, within Pf6N2E2_5470 at 6285.140 kb on + strand, within Pf6N2E2_5470 at 6285.140 kb on + strand, within Pf6N2E2_5470 at 6285.140 kb on + strand, within Pf6N2E2_5470 at 6285.141 kb on - strand, within Pf6N2E2_5470 at 6285.141 kb on - strand, within Pf6N2E2_5470 at 6285.141 kb on - strand, within Pf6N2E2_5470 at 6285.141 kb on - strand, within Pf6N2E2_5470 at 6285.141 kb on - strand, within Pf6N2E2_5470 at 6285.279 kb on - strand, within Pf6N2E2_5470 at 6285.302 kb on + strand, within Pf6N2E2_5470 at 6285.375 kb on - strand at 6285.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 6,283,275 - Pf6N2E2_5467 0.81 -0.3 6,283,275 - Pf6N2E2_5467 0.81 +0.9 6,283,685 + +0.1 6,283,685 + +1.1 6,283,685 + -1.2 6,283,685 + -0.6 6,283,686 - +0.7 6,283,691 + +0.4 6,283,692 - -0.7 6,283,692 - +0.3 6,283,763 - +0.6 6,283,763 - -1.6 6,283,976 - Pf6N2E2_5468 0.41 -0.1 6,284,046 - Pf6N2E2_5468 0.58 -0.2 6,284,062 + Pf6N2E2_5468 0.62 +0.2 6,284,191 - -0.7 6,284,206 + +0.8 6,284,206 + +0.1 6,284,206 + -0.1 6,284,207 - -0.6 6,284,207 - +0.8 6,284,207 - -0.5 6,284,207 - -0.4 6,284,207 - +0.3 6,284,210 + +0.3 6,284,211 - +0.3 6,284,211 - +0.3 6,284,211 - -0.9 6,284,211 - -0.6 6,284,297 - -0.9 6,284,297 - -0.2 6,284,322 + Pf6N2E2_5469 0.12 -0.0 6,284,322 + Pf6N2E2_5469 0.12 -0.7 6,284,322 + Pf6N2E2_5469 0.12 -0.5 6,284,322 + Pf6N2E2_5469 0.12 -0.4 6,284,322 + Pf6N2E2_5469 0.12 -0.7 6,284,322 + Pf6N2E2_5469 0.12 -1.1 6,284,323 - Pf6N2E2_5469 0.12 -0.8 6,284,323 - Pf6N2E2_5469 0.12 -1.3 6,284,323 - Pf6N2E2_5469 0.12 -0.9 6,284,323 - Pf6N2E2_5469 0.12 -0.5 6,284,461 - Pf6N2E2_5469 0.34 -1.8 6,284,467 + Pf6N2E2_5469 0.35 -3.4 6,284,467 + Pf6N2E2_5469 0.35 -0.8 6,284,634 + Pf6N2E2_5469 0.61 -0.4 6,284,634 + Pf6N2E2_5469 0.61 +0.1 6,284,634 + Pf6N2E2_5469 0.61 -1.3 6,284,634 + Pf6N2E2_5469 0.61 +0.4 6,284,634 + Pf6N2E2_5469 0.61 -0.9 6,284,635 - Pf6N2E2_5469 0.61 +0.1 6,284,697 - Pf6N2E2_5469 0.71 +0.2 6,284,918 + -0.2 6,284,942 + Pf6N2E2_5470 0.13 +0.6 6,284,942 + Pf6N2E2_5470 0.13 -1.3 6,284,942 + Pf6N2E2_5470 0.13 +0.5 6,285,039 + Pf6N2E2_5470 0.32 +0.8 6,285,039 + Pf6N2E2_5470 0.32 -1.1 6,285,039 + Pf6N2E2_5470 0.32 +0.5 6,285,040 - Pf6N2E2_5470 0.32 +0.4 6,285,040 - Pf6N2E2_5470 0.32 +0.6 6,285,040 - Pf6N2E2_5470 0.32 +0.0 6,285,040 - Pf6N2E2_5470 0.32 -0.2 6,285,040 - Pf6N2E2_5470 0.32 -0.2 6,285,140 + Pf6N2E2_5470 0.52 +0.6 6,285,140 + Pf6N2E2_5470 0.52 +0.4 6,285,140 + Pf6N2E2_5470 0.52 +0.0 6,285,141 - Pf6N2E2_5470 0.53 -0.7 6,285,141 - Pf6N2E2_5470 0.53 +0.6 6,285,141 - Pf6N2E2_5470 0.53 +1.7 6,285,141 - Pf6N2E2_5470 0.53 +0.0 6,285,141 - Pf6N2E2_5470 0.53 -0.7 6,285,279 - Pf6N2E2_5470 0.80 -2.0 6,285,302 + Pf6N2E2_5470 0.85 +0.4 6,285,375 - +0.3 6,285,375 - +0.2
Or see this region's nucleotide sequence