Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_491 and Pf6N2E2_492 are separated by 273 nucleotides Pf6N2E2_492 and Pf6N2E2_493 are separated by 55 nucleotides Pf6N2E2_493 and Pf6N2E2_494 are separated by 149 nucleotides Pf6N2E2_494 and Pf6N2E2_495 are separated by 29 nucleotides
Pf6N2E2_491 - hypothetical protein, at 558,482 to 558,835
_491
Pf6N2E2_492 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 559,109 to 559,729
_492
Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831
_493
Pf6N2E2_494 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 560,981 to 561,418
_494
Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563
_495
Position (kb) 559
560
561 Strain fitness (log2 ratio) -2
-1
0
1 at 558.796 kb on - strand, within Pf6N2E2_491 at 558.870 kb on - strand at 558.870 kb on - strand at 558.900 kb on - strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 559.018 kb on + strand at 559.018 kb on + strand at 559.019 kb on - strand at 559.141 kb on + strand at 559.142 kb on - strand at 559.256 kb on + strand, within Pf6N2E2_492 at 559.256 kb on + strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.750 kb on + strand at 559.751 kb on - strand at 559.751 kb on - strand at 559.758 kb on - strand at 560.132 kb on + strand, within Pf6N2E2_493 at 560.452 kb on + strand, within Pf6N2E2_493 at 560.452 kb on + strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.701 kb on - strand, within Pf6N2E2_493 at 560.729 kb on - strand at 560.729 kb on - strand at 560.729 kb on - strand at 560.894 kb on - strand at 560.896 kb on + strand at 560.908 kb on + strand at 560.909 kb on - strand at 560.909 kb on - strand at 560.927 kb on - strand at 560.946 kb on + strand at 560.946 kb on + strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.947 kb on - strand at 560.949 kb on + strand at 560.949 kb on + strand at 560.949 kb on + strand at 560.950 kb on - strand at 560.950 kb on - strand at 560.971 kb on + strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.972 kb on - strand at 560.973 kb on + strand at 560.973 kb on + strand at 561.065 kb on + strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.134 kb on - strand, within Pf6N2E2_494 at 561.180 kb on - strand, within Pf6N2E2_494 at 561.453 kb on - strand at 561.573 kb on + strand, within Pf6N2E2_495 at 561.637 kb on + strand, within Pf6N2E2_495
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 558,796 - Pf6N2E2_491 0.89 +0.3 558,870 - -0.9 558,870 - +0.3 558,900 - -0.6 558,998 + +0.9 558,998 + -0.3 558,998 + -1.1 558,999 - +1.2 558,999 - -0.4 558,999 - +0.5 558,999 - +0.3 558,999 - -0.5 559,018 + -0.0 559,018 + -0.3 559,019 - -0.1 559,141 + -0.6 559,142 - +0.3 559,256 + Pf6N2E2_492 0.24 +0.4 559,256 + Pf6N2E2_492 0.24 +1.0 559,257 - Pf6N2E2_492 0.24 +0.2 559,257 - Pf6N2E2_492 0.24 -1.4 559,259 + Pf6N2E2_492 0.24 +0.3 559,259 + Pf6N2E2_492 0.24 +0.3 559,260 - Pf6N2E2_492 0.24 -1.3 559,260 - Pf6N2E2_492 0.24 -0.4 559,260 - Pf6N2E2_492 0.24 -0.2 559,284 - Pf6N2E2_492 0.28 -0.6 559,284 - Pf6N2E2_492 0.28 -0.1 559,284 - Pf6N2E2_492 0.28 -0.5 559,284 - Pf6N2E2_492 0.28 +1.1 559,284 - Pf6N2E2_492 0.28 -0.1 559,750 + -0.9 559,751 - +0.4 559,751 - +1.2 559,758 - +0.7 560,132 + Pf6N2E2_493 0.33 -0.3 560,452 + Pf6N2E2_493 0.64 -0.1 560,452 + Pf6N2E2_493 0.64 -1.0 560,701 - Pf6N2E2_493 0.87 -1.1 560,701 - Pf6N2E2_493 0.87 -0.9 560,701 - Pf6N2E2_493 0.87 -0.5 560,701 - Pf6N2E2_493 0.87 -0.9 560,729 - +0.1 560,729 - -1.9 560,729 - +1.2 560,894 - +0.0 560,896 + +1.3 560,908 + +0.4 560,909 - -0.7 560,909 - -0.1 560,927 - -0.1 560,946 + +0.1 560,946 + +0.9 560,947 - -1.0 560,947 - -0.7 560,947 - +0.3 560,947 - +0.6 560,947 - -1.2 560,949 + +1.1 560,949 + -0.1 560,949 + +0.6 560,950 - -0.7 560,950 - -0.5 560,971 + -0.6 560,972 - +1.1 560,972 - +0.2 560,972 - -0.4 560,972 - +1.2 560,973 + +0.8 560,973 + +0.6 561,065 + Pf6N2E2_494 0.19 +1.6 561,134 - Pf6N2E2_494 0.35 -0.1 561,134 - Pf6N2E2_494 0.35 -0.0 561,134 - Pf6N2E2_494 0.35 -0.1 561,180 - Pf6N2E2_494 0.45 -0.9 561,453 - -0.8 561,573 + Pf6N2E2_495 0.11 +1.1 561,637 + Pf6N2E2_495 0.17 -0.5
Or see this region's nucleotide sequence