Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4426 and Pf6N2E2_4427 are separated by 225 nucleotides Pf6N2E2_4427 and Pf6N2E2_4428 are separated by 124 nucleotides Pf6N2E2_4428 and Pf6N2E2_4429 are separated by 101 nucleotides
Pf6N2E2_4426 - DNA-binding protein inhibitor Id-2-related protein, at 5,157,974 to 5,158,219
_4426
Pf6N2E2_4427 - FIG00954215: hypothetical protein, at 5,158,445 to 5,158,729
_4427
Pf6N2E2_4428 - Glutathione S-transferase N-terminal domain protein (EC 2.5.1.18), at 5,158,854 to 5,159,513
_4428
Pf6N2E2_4429 - Argininosuccinate lyase (EC 4.3.2.1), at 5,159,615 to 5,161,009
_4429
Position (kb)
5158
5159
5160 Strain fitness (log2 ratio)
-1
0
1
2 at 5157.911 kb on - strand at 5157.911 kb on - strand at 5157.998 kb on + strand at 5157.998 kb on + strand at 5157.998 kb on + strand at 5157.999 kb on - strand, within Pf6N2E2_4426 at 5157.999 kb on - strand, within Pf6N2E2_4426 at 5157.999 kb on - strand, within Pf6N2E2_4426 at 5157.999 kb on - strand, within Pf6N2E2_4426 at 5157.999 kb on - strand, within Pf6N2E2_4426 at 5158.016 kb on + strand, within Pf6N2E2_4426 at 5158.016 kb on + strand, within Pf6N2E2_4426 at 5158.016 kb on + strand, within Pf6N2E2_4426 at 5158.016 kb on + strand, within Pf6N2E2_4426 at 5158.219 kb on + strand at 5158.219 kb on + strand at 5158.219 kb on + strand at 5158.219 kb on + strand at 5158.219 kb on + strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.220 kb on - strand at 5158.238 kb on - strand at 5158.261 kb on + strand at 5158.261 kb on + strand at 5158.261 kb on + strand at 5158.261 kb on + strand at 5158.262 kb on - strand at 5158.262 kb on - strand at 5158.262 kb on - strand at 5158.262 kb on - strand at 5158.377 kb on + strand at 5158.377 kb on + strand at 5158.377 kb on + strand at 5158.377 kb on + strand at 5158.378 kb on - strand at 5158.378 kb on - strand at 5158.378 kb on - strand at 5158.446 kb on + strand at 5158.446 kb on + strand at 5158.494 kb on + strand, within Pf6N2E2_4427 at 5158.591 kb on - strand, within Pf6N2E2_4427 at 5158.761 kb on - strand at 5158.761 kb on - strand at 5158.761 kb on - strand at 5158.761 kb on - strand at 5158.797 kb on + strand at 5158.797 kb on + strand at 5158.798 kb on - strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.810 kb on + strand at 5158.811 kb on - strand at 5158.812 kb on + strand at 5158.883 kb on + strand at 5158.883 kb on + strand at 5158.884 kb on - strand at 5158.884 kb on - strand at 5158.887 kb on + strand at 5158.887 kb on + strand at 5158.887 kb on + strand at 5158.888 kb on - strand at 5158.890 kb on + strand at 5158.890 kb on + strand at 5158.890 kb on + strand at 5158.890 kb on + strand at 5158.890 kb on + strand at 5158.890 kb on + strand at 5158.891 kb on - strand at 5158.891 kb on - strand at 5158.891 kb on - strand at 5158.891 kb on - strand at 5158.891 kb on - strand at 5158.950 kb on + strand, within Pf6N2E2_4428 at 5158.950 kb on + strand, within Pf6N2E2_4428 at 5158.951 kb on - strand, within Pf6N2E2_4428 at 5159.028 kb on - strand, within Pf6N2E2_4428 at 5159.028 kb on - strand, within Pf6N2E2_4428 at 5159.055 kb on + strand, within Pf6N2E2_4428 at 5159.056 kb on - strand, within Pf6N2E2_4428 at 5159.056 kb on - strand, within Pf6N2E2_4428 at 5159.056 kb on - strand, within Pf6N2E2_4428 at 5159.148 kb on + strand, within Pf6N2E2_4428 at 5159.148 kb on + strand, within Pf6N2E2_4428 at 5159.148 kb on + strand, within Pf6N2E2_4428 at 5159.148 kb on + strand, within Pf6N2E2_4428 at 5159.148 kb on + strand, within Pf6N2E2_4428 at 5159.149 kb on - strand, within Pf6N2E2_4428 at 5159.175 kb on + strand, within Pf6N2E2_4428 at 5159.286 kb on + strand, within Pf6N2E2_4428 at 5159.287 kb on - strand, within Pf6N2E2_4428 at 5159.287 kb on - strand, within Pf6N2E2_4428 at 5159.322 kb on + strand, within Pf6N2E2_4428 at 5159.322 kb on + strand, within Pf6N2E2_4428 at 5159.323 kb on - strand, within Pf6N2E2_4428 at 5159.323 kb on - strand, within Pf6N2E2_4428 at 5159.323 kb on - strand, within Pf6N2E2_4428 at 5159.323 kb on - strand, within Pf6N2E2_4428 at 5159.331 kb on + strand, within Pf6N2E2_4428 at 5159.332 kb on - strand, within Pf6N2E2_4428 at 5159.332 kb on - strand, within Pf6N2E2_4428 at 5159.538 kb on + strand at 5159.572 kb on + strand at 5159.572 kb on + strand at 5159.572 kb on + strand at 5159.572 kb on + strand at 5159.573 kb on - strand at 5159.573 kb on - strand at 5159.573 kb on - strand at 5159.573 kb on - strand at 5159.573 kb on - strand at 5159.573 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,157,911 - +0.4 5,157,911 - -1.2 5,157,998 + +1.4 5,157,998 + +0.6 5,157,998 + +0.7 5,157,999 - Pf6N2E2_4426 0.10 -0.6 5,157,999 - Pf6N2E2_4426 0.10 +0.2 5,157,999 - Pf6N2E2_4426 0.10 +0.2 5,157,999 - Pf6N2E2_4426 0.10 -0.1 5,157,999 - Pf6N2E2_4426 0.10 +0.4 5,158,016 + Pf6N2E2_4426 0.17 -0.4 5,158,016 + Pf6N2E2_4426 0.17 -0.5 5,158,016 + Pf6N2E2_4426 0.17 -0.0 5,158,016 + Pf6N2E2_4426 0.17 +0.9 5,158,219 + +0.6 5,158,219 + -0.8 5,158,219 + -0.4 5,158,219 + -0.5 5,158,219 + -0.1 5,158,220 - +0.0 5,158,220 - -0.2 5,158,220 - +0.1 5,158,220 - +0.0 5,158,220 - +0.1 5,158,220 - +0.3 5,158,220 - +0.5 5,158,220 - +0.3 5,158,220 - +1.0 5,158,220 - -0.8 5,158,238 - +0.7 5,158,261 + +0.4 5,158,261 + -0.3 5,158,261 + +0.3 5,158,261 + +0.1 5,158,262 - +0.3 5,158,262 - +0.9 5,158,262 - -0.3 5,158,262 - -0.4 5,158,377 + -0.4 5,158,377 + +1.2 5,158,377 + +0.8 5,158,377 + +0.9 5,158,378 - +0.1 5,158,378 - -0.9 5,158,378 - -0.0 5,158,446 + +0.2 5,158,446 + +0.0 5,158,494 + Pf6N2E2_4427 0.17 +0.4 5,158,591 - Pf6N2E2_4427 0.51 +0.5 5,158,761 - -0.1 5,158,761 - -0.1 5,158,761 - +0.4 5,158,761 - -0.4 5,158,797 + +2.4 5,158,797 + +0.2 5,158,798 - -0.2 5,158,810 + +1.7 5,158,810 + -0.3 5,158,810 + +0.1 5,158,810 + +0.5 5,158,810 + +0.3 5,158,810 + +1.3 5,158,810 + +0.4 5,158,810 + -0.5 5,158,811 - +0.6 5,158,812 + +0.1 5,158,883 + +0.5 5,158,883 + -1.2 5,158,884 - +0.1 5,158,884 - +0.5 5,158,887 + -0.6 5,158,887 + +0.8 5,158,887 + +0.1 5,158,888 - +0.7 5,158,890 + +1.9 5,158,890 + +0.2 5,158,890 + +0.1 5,158,890 + -0.1 5,158,890 + -0.4 5,158,890 + +0.9 5,158,891 - -0.3 5,158,891 - +0.1 5,158,891 - +0.6 5,158,891 - +0.4 5,158,891 - +0.8 5,158,950 + Pf6N2E2_4428 0.15 +0.6 5,158,950 + Pf6N2E2_4428 0.15 -0.2 5,158,951 - Pf6N2E2_4428 0.15 +0.8 5,159,028 - Pf6N2E2_4428 0.26 +1.6 5,159,028 - Pf6N2E2_4428 0.26 +0.1 5,159,055 + Pf6N2E2_4428 0.30 -0.1 5,159,056 - Pf6N2E2_4428 0.31 -1.2 5,159,056 - Pf6N2E2_4428 0.31 +1.1 5,159,056 - Pf6N2E2_4428 0.31 +0.8 5,159,148 + Pf6N2E2_4428 0.45 +0.3 5,159,148 + Pf6N2E2_4428 0.45 +0.9 5,159,148 + Pf6N2E2_4428 0.45 +0.5 5,159,148 + Pf6N2E2_4428 0.45 +0.3 5,159,148 + Pf6N2E2_4428 0.45 +0.5 5,159,149 - Pf6N2E2_4428 0.45 +0.8 5,159,175 + Pf6N2E2_4428 0.49 -0.7 5,159,286 + Pf6N2E2_4428 0.65 +0.1 5,159,287 - Pf6N2E2_4428 0.66 +0.4 5,159,287 - Pf6N2E2_4428 0.66 -0.5 5,159,322 + Pf6N2E2_4428 0.71 +0.5 5,159,322 + Pf6N2E2_4428 0.71 +0.6 5,159,323 - Pf6N2E2_4428 0.71 -0.0 5,159,323 - Pf6N2E2_4428 0.71 +0.1 5,159,323 - Pf6N2E2_4428 0.71 -0.0 5,159,323 - Pf6N2E2_4428 0.71 -0.0 5,159,331 + Pf6N2E2_4428 0.72 +0.7 5,159,332 - Pf6N2E2_4428 0.72 +0.1 5,159,332 - Pf6N2E2_4428 0.72 +0.2 5,159,538 + +0.7 5,159,572 + -0.1 5,159,572 + +0.9 5,159,572 + -0.2 5,159,572 + +0.6 5,159,573 - +0.8 5,159,573 - -1.6 5,159,573 - +0.5 5,159,573 - +0.8 5,159,573 - -0.2 5,159,573 - +2.1
Or see this region's nucleotide sequence