Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3386 and Pf6N2E2_3387 are separated by 674 nucleotides Pf6N2E2_3387 and Pf6N2E2_3388 are separated by 435 nucleotides
Pf6N2E2_3386 - hypothetical protein, at 3,918,092 to 3,918,370
_3386
Pf6N2E2_3387 - Phage integrase, at 3,919,045 to 3,920,301
_3387
Pf6N2E2_3388 - putative integrase, at 3,920,737 to 3,921,714
_3388
Position (kb)
3919
3920
3921 Strain fitness (log2 ratio)
-1
0
1
2 at 3918.105 kb on + strand at 3918.105 kb on + strand at 3918.105 kb on + strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.111 kb on + strand at 3918.111 kb on + strand at 3918.112 kb on - strand at 3918.112 kb on - strand at 3918.172 kb on + strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.232 kb on + strand, within Pf6N2E2_3386 at 3918.233 kb on - strand, within Pf6N2E2_3386 at 3918.358 kb on + strand at 3918.358 kb on + strand at 3918.358 kb on + strand at 3918.359 kb on - strand at 3918.871 kb on - strand at 3918.997 kb on + strand at 3919.050 kb on + strand at 3919.050 kb on + strand at 3919.051 kb on - strand at 3919.105 kb on - strand at 3919.105 kb on - strand at 3919.105 kb on - strand at 3919.105 kb on - strand at 3919.119 kb on + strand at 3919.328 kb on + strand, within Pf6N2E2_3387 at 3919.328 kb on + strand, within Pf6N2E2_3387 at 3919.328 kb on + strand, within Pf6N2E2_3387 at 3919.329 kb on - strand, within Pf6N2E2_3387 at 3919.329 kb on - strand, within Pf6N2E2_3387 at 3919.329 kb on - strand, within Pf6N2E2_3387 at 3919.594 kb on + strand, within Pf6N2E2_3387 at 3919.595 kb on - strand, within Pf6N2E2_3387 at 3919.595 kb on - strand, within Pf6N2E2_3387 at 3919.595 kb on - strand, within Pf6N2E2_3387 at 3919.595 kb on - strand, within Pf6N2E2_3387 at 3919.595 kb on - strand, within Pf6N2E2_3387 at 3919.616 kb on + strand, within Pf6N2E2_3387 at 3919.617 kb on - strand, within Pf6N2E2_3387 at 3919.686 kb on - strand, within Pf6N2E2_3387 at 3919.803 kb on + strand, within Pf6N2E2_3387 at 3919.803 kb on + strand, within Pf6N2E2_3387 at 3919.896 kb on + strand, within Pf6N2E2_3387 at 3919.897 kb on - strand, within Pf6N2E2_3387 at 3919.954 kb on + strand, within Pf6N2E2_3387 at 3919.954 kb on + strand, within Pf6N2E2_3387 at 3919.954 kb on + strand, within Pf6N2E2_3387 at 3919.954 kb on + strand, within Pf6N2E2_3387 at 3919.954 kb on + strand, within Pf6N2E2_3387 at 3919.955 kb on - strand, within Pf6N2E2_3387 at 3919.955 kb on - strand, within Pf6N2E2_3387 at 3919.955 kb on - strand, within Pf6N2E2_3387 at 3919.971 kb on + strand, within Pf6N2E2_3387 at 3919.971 kb on + strand, within Pf6N2E2_3387 at 3919.971 kb on + strand, within Pf6N2E2_3387 at 3919.996 kb on - strand, within Pf6N2E2_3387 at 3920.045 kb on + strand, within Pf6N2E2_3387 at 3920.045 kb on + strand, within Pf6N2E2_3387 at 3920.046 kb on - strand, within Pf6N2E2_3387 at 3920.046 kb on - strand, within Pf6N2E2_3387 at 3920.046 kb on - strand, within Pf6N2E2_3387 at 3920.046 kb on - strand, within Pf6N2E2_3387 at 3920.161 kb on + strand, within Pf6N2E2_3387 at 3920.162 kb on - strand, within Pf6N2E2_3387 at 3920.176 kb on + strand at 3920.176 kb on + strand at 3920.177 kb on - strand at 3920.177 kb on - strand at 3920.177 kb on - strand at 3920.177 kb on - strand at 3920.177 kb on - strand at 3920.220 kb on + strand at 3920.220 kb on + strand at 3920.374 kb on - strand at 3920.384 kb on + strand at 3920.416 kb on + strand at 3920.417 kb on - strand at 3920.457 kb on + strand at 3920.466 kb on + strand at 3920.516 kb on + strand at 3920.523 kb on + strand at 3920.523 kb on + strand at 3920.523 kb on + strand at 3920.523 kb on + strand at 3920.524 kb on - strand at 3920.524 kb on - strand at 3920.524 kb on - strand at 3920.526 kb on + strand at 3920.526 kb on + strand at 3920.526 kb on + strand at 3920.526 kb on + strand at 3920.526 kb on + strand at 3920.526 kb on + strand at 3920.527 kb on - strand at 3920.527 kb on - strand at 3920.527 kb on - strand at 3920.527 kb on - strand at 3920.527 kb on - strand at 3920.527 kb on - strand at 3920.527 kb on - strand at 3920.529 kb on + strand at 3920.529 kb on + strand at 3920.529 kb on + strand at 3920.529 kb on + strand at 3920.530 kb on - strand at 3920.536 kb on + strand at 3920.536 kb on + strand at 3920.537 kb on - strand at 3920.537 kb on - strand at 3920.559 kb on + strand at 3920.560 kb on - strand at 3920.560 kb on - strand at 3920.560 kb on - strand at 3920.659 kb on + strand at 3920.660 kb on - strand at 3920.660 kb on - strand at 3920.753 kb on + strand at 3920.753 kb on + strand at 3920.753 kb on + strand at 3920.754 kb on - strand at 3920.754 kb on - strand at 3920.778 kb on + strand at 3920.778 kb on + strand at 3920.779 kb on - strand at 3920.779 kb on - strand at 3920.889 kb on - strand, within Pf6N2E2_3388 at 3920.903 kb on + strand, within Pf6N2E2_3388 at 3920.903 kb on + strand, within Pf6N2E2_3388 at 3920.904 kb on - strand, within Pf6N2E2_3388 at 3920.904 kb on - strand, within Pf6N2E2_3388 at 3920.980 kb on + strand, within Pf6N2E2_3388 at 3920.980 kb on + strand, within Pf6N2E2_3388 at 3920.981 kb on - strand, within Pf6N2E2_3388 at 3920.993 kb on + strand, within Pf6N2E2_3388 at 3920.994 kb on - strand, within Pf6N2E2_3388 at 3921.084 kb on + strand, within Pf6N2E2_3388 at 3921.084 kb on + strand, within Pf6N2E2_3388
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,918,105 + -0.1 3,918,105 + +0.0 3,918,105 + +0.1 3,918,106 - -0.8 3,918,106 - +0.3 3,918,106 - -0.2 3,918,106 - -0.8 3,918,111 + +1.0 3,918,111 + -1.0 3,918,112 - +0.8 3,918,112 - -0.8 3,918,172 + Pf6N2E2_3386 0.29 -0.7 3,918,173 - Pf6N2E2_3386 0.29 +0.5 3,918,173 - Pf6N2E2_3386 0.29 +0.0 3,918,173 - Pf6N2E2_3386 0.29 +0.7 3,918,210 - Pf6N2E2_3386 0.42 +0.1 3,918,210 - Pf6N2E2_3386 0.42 +0.8 3,918,210 - Pf6N2E2_3386 0.42 +0.1 3,918,232 + Pf6N2E2_3386 0.50 +1.2 3,918,233 - Pf6N2E2_3386 0.51 +0.0 3,918,358 + +0.4 3,918,358 + -0.5 3,918,358 + -0.7 3,918,359 - -0.2 3,918,871 - -0.4 3,918,997 + -1.2 3,919,050 + -0.5 3,919,050 + +0.3 3,919,051 - -0.5 3,919,105 - +1.4 3,919,105 - +0.5 3,919,105 - +0.5 3,919,105 - -0.4 3,919,119 + +0.8 3,919,328 + Pf6N2E2_3387 0.23 -0.9 3,919,328 + Pf6N2E2_3387 0.23 +1.0 3,919,328 + Pf6N2E2_3387 0.23 +0.7 3,919,329 - Pf6N2E2_3387 0.23 +1.3 3,919,329 - Pf6N2E2_3387 0.23 +2.1 3,919,329 - Pf6N2E2_3387 0.23 +0.2 3,919,594 + Pf6N2E2_3387 0.44 -0.4 3,919,595 - Pf6N2E2_3387 0.44 +0.5 3,919,595 - Pf6N2E2_3387 0.44 +0.5 3,919,595 - Pf6N2E2_3387 0.44 +0.8 3,919,595 - Pf6N2E2_3387 0.44 +0.9 3,919,595 - Pf6N2E2_3387 0.44 +0.4 3,919,616 + Pf6N2E2_3387 0.45 +0.8 3,919,617 - Pf6N2E2_3387 0.46 +1.2 3,919,686 - Pf6N2E2_3387 0.51 +0.8 3,919,803 + Pf6N2E2_3387 0.60 -0.0 3,919,803 + Pf6N2E2_3387 0.60 +0.7 3,919,896 + Pf6N2E2_3387 0.68 +0.8 3,919,897 - Pf6N2E2_3387 0.68 -0.6 3,919,954 + Pf6N2E2_3387 0.72 -0.1 3,919,954 + Pf6N2E2_3387 0.72 +0.3 3,919,954 + Pf6N2E2_3387 0.72 +0.1 3,919,954 + Pf6N2E2_3387 0.72 +1.2 3,919,954 + Pf6N2E2_3387 0.72 +0.2 3,919,955 - Pf6N2E2_3387 0.72 +0.4 3,919,955 - Pf6N2E2_3387 0.72 -0.3 3,919,955 - Pf6N2E2_3387 0.72 +1.4 3,919,971 + Pf6N2E2_3387 0.74 +0.9 3,919,971 + Pf6N2E2_3387 0.74 +0.2 3,919,971 + Pf6N2E2_3387 0.74 +0.8 3,919,996 - Pf6N2E2_3387 0.76 +0.1 3,920,045 + Pf6N2E2_3387 0.80 +0.9 3,920,045 + Pf6N2E2_3387 0.80 +0.9 3,920,046 - Pf6N2E2_3387 0.80 -0.3 3,920,046 - Pf6N2E2_3387 0.80 +0.8 3,920,046 - Pf6N2E2_3387 0.80 +1.0 3,920,046 - Pf6N2E2_3387 0.80 -0.1 3,920,161 + Pf6N2E2_3387 0.89 -0.6 3,920,162 - Pf6N2E2_3387 0.89 +0.5 3,920,176 + -0.7 3,920,176 + +0.5 3,920,177 - +0.3 3,920,177 - +2.1 3,920,177 - -0.2 3,920,177 - +2.3 3,920,177 - -1.6 3,920,220 + -0.7 3,920,220 + +0.3 3,920,374 - +0.1 3,920,384 + +0.5 3,920,416 + -0.9 3,920,417 - -0.2 3,920,457 + -0.3 3,920,466 + -0.9 3,920,516 + -0.5 3,920,523 + +0.7 3,920,523 + +0.8 3,920,523 + +0.8 3,920,523 + +1.6 3,920,524 - -0.5 3,920,524 - +0.4 3,920,524 - +0.3 3,920,526 + -0.9 3,920,526 + +0.2 3,920,526 + -0.3 3,920,526 + -0.4 3,920,526 + +0.4 3,920,526 + -0.8 3,920,527 - +0.6 3,920,527 - -0.6 3,920,527 - -0.6 3,920,527 - -0.2 3,920,527 - +0.3 3,920,527 - +1.3 3,920,527 - +0.0 3,920,529 + +0.2 3,920,529 + +0.4 3,920,529 + -0.5 3,920,529 + -1.0 3,920,530 - +0.2 3,920,536 + -0.3 3,920,536 + -1.0 3,920,537 - -0.5 3,920,537 - +0.7 3,920,559 + +0.7 3,920,560 - -0.1 3,920,560 - -0.0 3,920,560 - +0.2 3,920,659 + -1.0 3,920,660 - -0.2 3,920,660 - -0.6 3,920,753 + -0.8 3,920,753 + -0.2 3,920,753 + +0.8 3,920,754 - +0.4 3,920,754 - +0.3 3,920,778 + +1.1 3,920,778 + +0.5 3,920,779 - +0.4 3,920,779 - -0.1 3,920,889 - Pf6N2E2_3388 0.16 +1.4 3,920,903 + Pf6N2E2_3388 0.17 -0.0 3,920,903 + Pf6N2E2_3388 0.17 +0.0 3,920,904 - Pf6N2E2_3388 0.17 +0.8 3,920,904 - Pf6N2E2_3388 0.17 +0.0 3,920,980 + Pf6N2E2_3388 0.25 -0.1 3,920,980 + Pf6N2E2_3388 0.25 +0.7 3,920,981 - Pf6N2E2_3388 0.25 +0.0 3,920,993 + Pf6N2E2_3388 0.26 -1.4 3,920,994 - Pf6N2E2_3388 0.26 -0.1 3,921,084 + Pf6N2E2_3388 0.35 -0.8 3,921,084 + Pf6N2E2_3388 0.35 +0.5
Or see this region's nucleotide sequence