Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2772 and Pf6N2E2_2773 are separated by 131 nucleotides Pf6N2E2_2773 and Pf6N2E2_2774 are separated by 64 nucleotides Pf6N2E2_2774 and Pf6N2E2_2775 are separated by 800 nucleotides
Pf6N2E2_2772 - Uracil-DNA glycosylase, family 1, at 3,260,516 to 3,261,208
_2772
Pf6N2E2_2773 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,261,340 to 3,262,452
_2773
Pf6N2E2_2774 - hypothetical protein, at 3,262,517 to 3,262,639
_2774
Pf6N2E2_2775 - Urea carboxylase-related ABC transporter, periplasmic substrate-binding protein, at 3,263,440 to 3,264,507
_2775
Position (kb)
3261
3262
3263 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.518 kb on - strand at 3260.705 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.822 kb on - strand, within Pf6N2E2_2772 at 3260.822 kb on - strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.917 kb on + strand, within Pf6N2E2_2772 at 3261.100 kb on - strand, within Pf6N2E2_2772 at 3261.100 kb on - strand, within Pf6N2E2_2772 at 3261.111 kb on - strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.191 kb on - strand at 3261.251 kb on + strand at 3261.251 kb on + strand at 3261.251 kb on + strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.307 kb on + strand at 3261.308 kb on - strand at 3261.314 kb on + strand at 3261.314 kb on + strand at 3261.315 kb on - strand at 3261.315 kb on - strand at 3261.315 kb on - strand at 3261.503 kb on + strand, within Pf6N2E2_2773 at 3261.503 kb on + strand, within Pf6N2E2_2773 at 3261.503 kb on + strand, within Pf6N2E2_2773 at 3261.561 kb on - strand, within Pf6N2E2_2773 at 3261.590 kb on + strand, within Pf6N2E2_2773 at 3261.625 kb on + strand, within Pf6N2E2_2773 at 3261.652 kb on + strand, within Pf6N2E2_2773 at 3261.653 kb on - strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.686 kb on - strand, within Pf6N2E2_2773 at 3261.686 kb on - strand, within Pf6N2E2_2773 at 3261.686 kb on - strand, within Pf6N2E2_2773 at 3261.689 kb on + strand at 3261.689 kb on + strand, within Pf6N2E2_2773 at 3261.689 kb on + strand, within Pf6N2E2_2773 at 3261.690 kb on - strand, within Pf6N2E2_2773 at 3261.690 kb on - strand, within Pf6N2E2_2773 at 3261.690 kb on - strand, within Pf6N2E2_2773 at 3261.727 kb on - strand, within Pf6N2E2_2773 at 3262.196 kb on - strand, within Pf6N2E2_2773 at 3262.261 kb on + strand, within Pf6N2E2_2773 at 3262.261 kb on + strand, within Pf6N2E2_2773 at 3262.261 kb on + strand, within Pf6N2E2_2773 at 3262.261 kb on + strand, within Pf6N2E2_2773 at 3262.261 kb on + strand, within Pf6N2E2_2773 at 3262.262 kb on - strand, within Pf6N2E2_2773 at 3262.262 kb on - strand, within Pf6N2E2_2773 at 3262.262 kb on - strand, within Pf6N2E2_2773 at 3262.478 kb on - strand at 3262.492 kb on - strand at 3262.492 kb on - strand at 3262.492 kb on - strand at 3262.696 kb on + strand at 3262.697 kb on - strand at 3262.697 kb on - strand at 3262.700 kb on - strand at 3262.717 kb on + strand at 3262.717 kb on + strand at 3262.717 kb on + strand at 3262.717 kb on + strand at 3262.721 kb on - strand at 3262.721 kb on - strand at 3262.735 kb on + strand at 3262.736 kb on - strand at 3262.756 kb on + strand at 3262.756 kb on + strand at 3262.756 kb on + strand at 3262.757 kb on - strand at 3262.759 kb on + strand at 3262.759 kb on + strand at 3262.777 kb on + strand at 3262.778 kb on - strand at 3262.778 kb on - strand at 3262.778 kb on - strand at 3262.795 kb on + strand at 3262.795 kb on + strand at 3262.795 kb on + strand at 3262.796 kb on - strand at 3262.810 kb on + strand at 3262.810 kb on + strand at 3262.811 kb on - strand at 3262.811 kb on - strand at 3262.811 kb on - strand at 3262.835 kb on - strand at 3262.835 kb on - strand at 3262.835 kb on - strand at 3262.849 kb on + strand at 3262.849 kb on + strand at 3262.849 kb on + strand at 3262.850 kb on - strand at 3262.850 kb on - strand at 3262.852 kb on + strand at 3262.852 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.871 kb on + strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.872 kb on - strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.873 kb on + strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.874 kb on - strand at 3262.888 kb on + strand at 3262.889 kb on - strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.891 kb on + strand at 3262.892 kb on - strand at 3262.892 kb on - strand at 3262.892 kb on - strand at 3262.909 kb on + strand at 3262.910 kb on - strand at 3262.912 kb on + strand at 3262.913 kb on - strand at 3262.969 kb on + strand at 3262.970 kb on - strand at 3262.970 kb on - strand at 3262.970 kb on - strand at 3263.050 kb on - strand at 3263.050 kb on - strand at 3263.094 kb on - strand at 3263.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,260,517 + -0.1 3,260,517 + +0.6 3,260,517 + -0.4 3,260,517 + -0.4 3,260,518 - +0.3 3,260,705 + Pf6N2E2_2772 0.27 -0.6 3,260,821 + Pf6N2E2_2772 0.44 +0.4 3,260,821 + Pf6N2E2_2772 0.44 -0.0 3,260,821 + Pf6N2E2_2772 0.44 -0.1 3,260,822 - Pf6N2E2_2772 0.44 -0.6 3,260,822 - Pf6N2E2_2772 0.44 -1.0 3,260,913 + Pf6N2E2_2772 0.57 +0.0 3,260,913 + Pf6N2E2_2772 0.57 +0.8 3,260,913 + Pf6N2E2_2772 0.57 -2.1 3,260,913 + Pf6N2E2_2772 0.57 -0.2 3,260,914 - Pf6N2E2_2772 0.57 +0.5 3,260,914 - Pf6N2E2_2772 0.57 -0.4 3,260,914 - Pf6N2E2_2772 0.57 +1.6 3,260,914 - Pf6N2E2_2772 0.57 +0.1 3,260,917 + Pf6N2E2_2772 0.58 -0.5 3,261,100 - Pf6N2E2_2772 0.84 +0.6 3,261,100 - Pf6N2E2_2772 0.84 +0.3 3,261,111 - Pf6N2E2_2772 0.86 +0.2 3,261,135 + Pf6N2E2_2772 0.89 +0.9 3,261,135 + Pf6N2E2_2772 0.89 -1.0 3,261,135 + Pf6N2E2_2772 0.89 -0.0 3,261,135 + Pf6N2E2_2772 0.89 +0.1 3,261,135 + Pf6N2E2_2772 0.89 +0.2 3,261,136 - Pf6N2E2_2772 0.89 -0.9 3,261,136 - Pf6N2E2_2772 0.89 +0.1 3,261,136 - Pf6N2E2_2772 0.89 +0.5 3,261,136 - Pf6N2E2_2772 0.89 +0.3 3,261,191 - -0.8 3,261,251 + -1.2 3,261,251 + +0.4 3,261,251 + +0.3 3,261,252 - -0.3 3,261,252 - -0.2 3,261,252 - +0.7 3,261,252 - -0.4 3,261,252 - -0.3 3,261,307 + +0.8 3,261,308 - +0.2 3,261,314 + +0.2 3,261,314 + +0.4 3,261,315 - +0.4 3,261,315 - +0.3 3,261,315 - -0.2 3,261,503 + Pf6N2E2_2773 0.15 +0.7 3,261,503 + Pf6N2E2_2773 0.15 +0.2 3,261,503 + Pf6N2E2_2773 0.15 +0.1 3,261,561 - Pf6N2E2_2773 0.20 +1.2 3,261,590 + Pf6N2E2_2773 0.22 +0.6 3,261,625 + Pf6N2E2_2773 0.26 +0.3 3,261,652 + Pf6N2E2_2773 0.28 +0.1 3,261,653 - Pf6N2E2_2773 0.28 +0.2 3,261,685 + Pf6N2E2_2773 0.31 +1.4 3,261,685 + Pf6N2E2_2773 0.31 -0.1 3,261,685 + Pf6N2E2_2773 0.31 +0.4 3,261,685 + Pf6N2E2_2773 0.31 -0.3 3,261,685 + Pf6N2E2_2773 0.31 +0.8 3,261,685 + Pf6N2E2_2773 0.31 +0.4 3,261,686 - Pf6N2E2_2773 0.31 -0.1 3,261,686 - Pf6N2E2_2773 0.31 +0.3 3,261,686 - Pf6N2E2_2773 0.31 -0.3 3,261,689 + -0.7 3,261,689 + Pf6N2E2_2773 0.31 -0.0 3,261,689 + Pf6N2E2_2773 0.31 -0.2 3,261,690 - Pf6N2E2_2773 0.31 +0.6 3,261,690 - Pf6N2E2_2773 0.31 +1.0 3,261,690 - Pf6N2E2_2773 0.31 -0.2 3,261,727 - Pf6N2E2_2773 0.35 +0.7 3,262,196 - Pf6N2E2_2773 0.77 -0.3 3,262,261 + Pf6N2E2_2773 0.83 -0.7 3,262,261 + Pf6N2E2_2773 0.83 -0.0 3,262,261 + Pf6N2E2_2773 0.83 +0.3 3,262,261 + Pf6N2E2_2773 0.83 +0.6 3,262,261 + Pf6N2E2_2773 0.83 +0.5 3,262,262 - Pf6N2E2_2773 0.83 -2.3 3,262,262 - Pf6N2E2_2773 0.83 +0.9 3,262,262 - Pf6N2E2_2773 0.83 -0.4 3,262,478 - +1.2 3,262,492 - -0.3 3,262,492 - -0.2 3,262,492 - +0.4 3,262,696 + -0.2 3,262,697 - -0.6 3,262,697 - -0.3 3,262,700 - -0.9 3,262,717 + +0.6 3,262,717 + +1.1 3,262,717 + -0.2 3,262,717 + +1.1 3,262,721 - -0.5 3,262,721 - +0.2 3,262,735 + +0.2 3,262,736 - -0.9 3,262,756 + +0.0 3,262,756 + -1.7 3,262,756 + +0.9 3,262,757 - +0.0 3,262,759 + +0.4 3,262,759 + +1.4 3,262,777 + -0.0 3,262,778 - -0.1 3,262,778 - +0.2 3,262,778 - -0.5 3,262,795 + -0.2 3,262,795 + +0.1 3,262,795 + -1.3 3,262,796 - -0.4 3,262,810 + +0.9 3,262,810 + -0.7 3,262,811 - -0.1 3,262,811 - +0.9 3,262,811 - -0.3 3,262,835 - +0.6 3,262,835 - -0.5 3,262,835 - +0.0 3,262,849 + +0.3 3,262,849 + +0.3 3,262,849 + +0.2 3,262,850 - +0.1 3,262,850 - +0.8 3,262,852 + +0.4 3,262,852 + -1.4 3,262,871 + -0.0 3,262,871 + +0.6 3,262,871 + +0.3 3,262,871 + +0.7 3,262,871 + -0.4 3,262,871 + +0.6 3,262,872 - +0.4 3,262,872 - -0.4 3,262,872 - -0.3 3,262,872 - -0.3 3,262,872 - +0.3 3,262,872 - -0.4 3,262,872 - +0.6 3,262,873 + +0.3 3,262,873 + +1.5 3,262,873 + -1.3 3,262,873 + +2.2 3,262,873 + -0.7 3,262,873 + +0.8 3,262,874 - +1.1 3,262,874 - -2.5 3,262,874 - +0.1 3,262,874 - -0.1 3,262,874 - -0.0 3,262,874 - +0.2 3,262,874 - -0.0 3,262,888 + -0.6 3,262,889 - -0.3 3,262,891 + -0.1 3,262,891 + +0.7 3,262,891 + -0.7 3,262,891 + +1.3 3,262,891 + +0.7 3,262,892 - +0.2 3,262,892 - +1.3 3,262,892 - +0.6 3,262,909 + +2.1 3,262,910 - +0.3 3,262,912 + +0.3 3,262,913 - -0.7 3,262,969 + +0.2 3,262,970 - +0.1 3,262,970 - -2.1 3,262,970 - -0.1 3,263,050 - +0.0 3,263,050 - +0.9 3,263,094 - +0.6 3,263,353 - +0.1
Or see this region's nucleotide sequence