Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2146 and Pf6N2E2_2147 are separated by 115 nucleotides Pf6N2E2_2147 and Pf6N2E2_2148 overlap by 4 nucleotides Pf6N2E2_2148 and Pf6N2E2_2149 are separated by 297 nucleotides
Pf6N2E2_2146 - Porphobilinogen synthase (EC 4.2.1.24), at 2,551,988 to 2,552,962
_2146
Pf6N2E2_2147 - Phenazine biosynthesis protein PhzF like, at 2,553,078 to 2,553,935
_2147
Pf6N2E2_2148 - Glutathione S-transferase (EC 2.5.1.18), at 2,553,932 to 2,554,630
_2148
Pf6N2E2_2149 - contains type I hydrophobic transmembrane region and ATP/GTP binding motif, at 2,554,928 to 2,555,623
_2149
Position (kb)
2553
2554 Strain fitness (log2 ratio)
-2
-1
0
1 at 2552.148 kb on + strand, within Pf6N2E2_2146 at 2552.149 kb on - strand, within Pf6N2E2_2146 at 2552.202 kb on + strand, within Pf6N2E2_2146 at 2552.203 kb on - strand, within Pf6N2E2_2146 at 2552.203 kb on - strand, within Pf6N2E2_2146 at 2552.355 kb on + strand, within Pf6N2E2_2146 at 2552.356 kb on - strand, within Pf6N2E2_2146 at 2552.356 kb on - strand, within Pf6N2E2_2146 at 2552.382 kb on + strand, within Pf6N2E2_2146 at 2552.383 kb on - strand, within Pf6N2E2_2146 at 2552.586 kb on + strand, within Pf6N2E2_2146 at 2552.754 kb on + strand, within Pf6N2E2_2146 at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.981 kb on - strand at 2553.008 kb on + strand at 2553.008 kb on + strand at 2553.009 kb on - strand at 2553.034 kb on + strand at 2553.035 kb on - strand at 2553.057 kb on - strand at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.728 kb on + strand, within Pf6N2E2_2147 at 2553.729 kb on - strand, within Pf6N2E2_2147 at 2553.904 kb on + strand at 2553.904 kb on + strand at 2553.904 kb on + strand at 2553.905 kb on - strand at 2553.905 kb on - strand at 2553.905 kb on - strand at 2554.042 kb on - strand, within Pf6N2E2_2148 at 2554.096 kb on - strand, within Pf6N2E2_2148 at 2554.173 kb on + strand, within Pf6N2E2_2148 at 2554.173 kb on + strand, within Pf6N2E2_2148 at 2554.173 kb on + strand, within Pf6N2E2_2148 at 2554.174 kb on - strand, within Pf6N2E2_2148 at 2554.174 kb on - strand, within Pf6N2E2_2148 at 2554.200 kb on + strand, within Pf6N2E2_2148 at 2554.344 kb on + strand, within Pf6N2E2_2148 at 2554.560 kb on + strand, within Pf6N2E2_2148 at 2554.560 kb on + strand, within Pf6N2E2_2148 at 2554.561 kb on - strand at 2554.678 kb on + strand at 2554.678 kb on + strand at 2554.678 kb on + strand at 2554.679 kb on - strand at 2554.707 kb on - strand at 2554.759 kb on + strand at 2554.759 kb on + strand at 2554.760 kb on - strand at 2554.779 kb on + strand at 2554.779 kb on + strand at 2554.780 kb on - strand at 2554.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,552,148 + Pf6N2E2_2146 0.16 +0.1 2,552,149 - Pf6N2E2_2146 0.17 -0.3 2,552,202 + Pf6N2E2_2146 0.22 -0.5 2,552,203 - Pf6N2E2_2146 0.22 -0.9 2,552,203 - Pf6N2E2_2146 0.22 +0.4 2,552,355 + Pf6N2E2_2146 0.38 +1.0 2,552,356 - Pf6N2E2_2146 0.38 -0.2 2,552,356 - Pf6N2E2_2146 0.38 -0.5 2,552,382 + Pf6N2E2_2146 0.40 -0.4 2,552,383 - Pf6N2E2_2146 0.41 +0.4 2,552,586 + Pf6N2E2_2146 0.61 +0.7 2,552,754 + Pf6N2E2_2146 0.79 +0.2 2,552,980 + +0.5 2,552,980 + +0.4 2,552,980 + +0.7 2,552,980 + -0.4 2,552,980 + +0.1 2,552,981 - -0.4 2,553,008 + +0.2 2,553,008 + -1.0 2,553,009 - -0.1 2,553,034 + +0.5 2,553,035 - +0.6 2,553,057 - +0.6 2,553,199 + Pf6N2E2_2147 0.14 -0.5 2,553,199 + Pf6N2E2_2147 0.14 -0.6 2,553,199 + Pf6N2E2_2147 0.14 +0.3 2,553,199 + Pf6N2E2_2147 0.14 -1.3 2,553,199 + Pf6N2E2_2147 0.14 +0.7 2,553,199 + Pf6N2E2_2147 0.14 -1.4 2,553,199 + Pf6N2E2_2147 0.14 -1.1 2,553,199 + Pf6N2E2_2147 0.14 -0.3 2,553,199 + Pf6N2E2_2147 0.14 -1.0 2,553,199 + Pf6N2E2_2147 0.14 +0.0 2,553,199 + Pf6N2E2_2147 0.14 +1.0 2,553,200 - Pf6N2E2_2147 0.14 -0.5 2,553,200 - Pf6N2E2_2147 0.14 +0.2 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -1.3 2,553,200 - Pf6N2E2_2147 0.14 +1.1 2,553,200 - Pf6N2E2_2147 0.14 -0.4 2,553,200 - Pf6N2E2_2147 0.14 +0.5 2,553,200 - Pf6N2E2_2147 0.14 +1.0 2,553,200 - Pf6N2E2_2147 0.14 -1.3 2,553,200 - Pf6N2E2_2147 0.14 +0.4 2,553,200 - Pf6N2E2_2147 0.14 +0.0 2,553,200 - Pf6N2E2_2147 0.14 +1.0 2,553,200 - Pf6N2E2_2147 0.14 +0.5 2,553,200 - Pf6N2E2_2147 0.14 -0.5 2,553,200 - Pf6N2E2_2147 0.14 -0.4 2,553,200 - Pf6N2E2_2147 0.14 -0.6 2,553,200 - Pf6N2E2_2147 0.14 -0.0 2,553,200 - Pf6N2E2_2147 0.14 +0.4 2,553,728 + Pf6N2E2_2147 0.76 -0.5 2,553,729 - Pf6N2E2_2147 0.76 +0.6 2,553,904 + +1.1 2,553,904 + -0.7 2,553,904 + -0.4 2,553,905 - +0.9 2,553,905 - -0.0 2,553,905 - +0.5 2,554,042 - Pf6N2E2_2148 0.16 +0.9 2,554,096 - Pf6N2E2_2148 0.23 +0.5 2,554,173 + Pf6N2E2_2148 0.34 -1.6 2,554,173 + Pf6N2E2_2148 0.34 +1.4 2,554,173 + Pf6N2E2_2148 0.34 +0.4 2,554,174 - Pf6N2E2_2148 0.35 +0.7 2,554,174 - Pf6N2E2_2148 0.35 +0.8 2,554,200 + Pf6N2E2_2148 0.38 +0.6 2,554,344 + Pf6N2E2_2148 0.59 +0.7 2,554,560 + Pf6N2E2_2148 0.90 -0.7 2,554,560 + Pf6N2E2_2148 0.90 -1.3 2,554,561 - -0.1 2,554,678 + -0.3 2,554,678 + -0.3 2,554,678 + +1.5 2,554,679 - +0.8 2,554,707 - +1.1 2,554,759 + +1.5 2,554,759 + +0.8 2,554,760 - +1.3 2,554,779 + -2.1 2,554,779 + +1.0 2,554,780 - -0.5 2,554,780 - -1.4
Or see this region's nucleotide sequence