Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1933 and Pf6N2E2_1934 are separated by 55 nucleotides Pf6N2E2_1934 and Pf6N2E2_1935 are separated by 63 nucleotides Pf6N2E2_1935 and Pf6N2E2_1936 are separated by 142 nucleotides
Pf6N2E2_1933 - TcuR: regulates tcuABC genes used in utilization of tricarballylate, at 2,289,767 to 2,290,699
_1933
Pf6N2E2_1934 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,290,755 to 2,291,549
_1934
Pf6N2E2_1935 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,291,613 to 2,292,257
_1935
Pf6N2E2_1936 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 2,292,400 to 2,293,440
_1936
Position (kb)
2290
2291
2292 Strain fitness (log2 ratio)
-2
-1
0
1 at 2289.823 kb on + strand at 2289.824 kb on - strand at 2289.824 kb on - strand at 2289.956 kb on - strand, within Pf6N2E2_1933 at 2289.980 kb on - strand, within Pf6N2E2_1933 at 2290.127 kb on + strand, within Pf6N2E2_1933 at 2290.128 kb on - strand, within Pf6N2E2_1933 at 2290.128 kb on - strand, within Pf6N2E2_1933 at 2290.130 kb on - strand, within Pf6N2E2_1933 at 2290.223 kb on + strand, within Pf6N2E2_1933 at 2290.336 kb on + strand, within Pf6N2E2_1933 at 2290.337 kb on - strand, within Pf6N2E2_1933 at 2290.553 kb on + strand, within Pf6N2E2_1933 at 2290.664 kb on + strand at 2290.713 kb on + strand at 2290.713 kb on + strand at 2290.757 kb on - strand at 2290.792 kb on + strand at 2290.792 kb on + strand at 2290.793 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.894 kb on + strand, within Pf6N2E2_1934 at 2290.895 kb on - strand, within Pf6N2E2_1934 at 2291.010 kb on + strand, within Pf6N2E2_1934 at 2291.011 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.083 kb on - strand, within Pf6N2E2_1934 at 2291.098 kb on + strand, within Pf6N2E2_1934 at 2291.099 kb on - strand, within Pf6N2E2_1934 at 2291.135 kb on + strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.137 kb on + strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.266 kb on - strand, within Pf6N2E2_1934 at 2291.304 kb on - strand, within Pf6N2E2_1934 at 2291.500 kb on - strand at 2291.509 kb on - strand at 2291.529 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.791 kb on - strand, within Pf6N2E2_1935 at 2291.889 kb on + strand, within Pf6N2E2_1935 at 2291.890 kb on - strand, within Pf6N2E2_1935 at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.281 kb on + strand at 2292.282 kb on - strand at 2292.282 kb on - strand at 2292.334 kb on - strand at 2292.401 kb on + strand at 2292.521 kb on + strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,289,823 + +0.4 2,289,824 - +1.1 2,289,824 - -0.5 2,289,956 - Pf6N2E2_1933 0.20 +1.3 2,289,980 - Pf6N2E2_1933 0.23 +0.5 2,290,127 + Pf6N2E2_1933 0.39 -1.4 2,290,128 - Pf6N2E2_1933 0.39 -1.2 2,290,128 - Pf6N2E2_1933 0.39 +0.5 2,290,130 - Pf6N2E2_1933 0.39 -1.0 2,290,223 + Pf6N2E2_1933 0.49 +0.4 2,290,336 + Pf6N2E2_1933 0.61 -0.4 2,290,337 - Pf6N2E2_1933 0.61 +0.4 2,290,553 + Pf6N2E2_1933 0.84 +0.1 2,290,664 + +0.2 2,290,713 + +0.4 2,290,713 + +1.1 2,290,757 - +0.6 2,290,792 + +0.0 2,290,792 + +0.7 2,290,793 - -0.8 2,290,814 - -1.5 2,290,814 - -1.0 2,290,814 - -0.4 2,290,814 - +0.4 2,290,894 + Pf6N2E2_1934 0.17 -1.2 2,290,895 - Pf6N2E2_1934 0.18 -1.9 2,291,010 + Pf6N2E2_1934 0.32 -0.5 2,291,011 - Pf6N2E2_1934 0.32 -1.4 2,291,056 - Pf6N2E2_1934 0.38 -1.7 2,291,056 - Pf6N2E2_1934 0.38 -1.5 2,291,083 - Pf6N2E2_1934 0.41 -0.4 2,291,098 + Pf6N2E2_1934 0.43 +0.5 2,291,099 - Pf6N2E2_1934 0.43 -0.0 2,291,135 + Pf6N2E2_1934 0.48 +0.5 2,291,136 - Pf6N2E2_1934 0.48 +0.0 2,291,136 - Pf6N2E2_1934 0.48 +0.4 2,291,137 + Pf6N2E2_1934 0.48 -0.2 2,291,138 - Pf6N2E2_1934 0.48 -0.2 2,291,138 - Pf6N2E2_1934 0.48 -1.9 2,291,138 - Pf6N2E2_1934 0.48 +1.0 2,291,138 - Pf6N2E2_1934 0.48 -0.2 2,291,138 - Pf6N2E2_1934 0.48 +0.1 2,291,266 - Pf6N2E2_1934 0.64 +0.3 2,291,304 - Pf6N2E2_1934 0.69 +0.5 2,291,500 - -1.1 2,291,509 - +1.3 2,291,529 - -0.1 2,291,553 - +0.3 2,291,553 - +0.6 2,291,553 - -0.1 2,291,553 - +0.6 2,291,553 - -0.6 2,291,553 - +0.3 2,291,790 + Pf6N2E2_1935 0.27 -0.4 2,291,790 + Pf6N2E2_1935 0.27 +0.5 2,291,791 - Pf6N2E2_1935 0.28 +0.4 2,291,889 + Pf6N2E2_1935 0.43 -0.6 2,291,890 - Pf6N2E2_1935 0.43 -0.2 2,292,234 - +0.0 2,292,234 - -0.0 2,292,234 - -0.5 2,292,281 + -0.0 2,292,282 - +0.2 2,292,282 - -0.9 2,292,334 - +0.0 2,292,401 + +0.6 2,292,521 + Pf6N2E2_1936 0.12 +0.7 2,292,522 - Pf6N2E2_1936 0.12 -1.5 2,292,522 - Pf6N2E2_1936 0.12 +0.1
Or see this region's nucleotide sequence