Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1045 and Pf6N2E2_1046 are separated by 624 nucleotides Pf6N2E2_1046 and Pf6N2E2_1047 are separated by 25 nucleotides
Pf6N2E2_1045 - hypothetical protein, at 1,219,379 to 1,219,516
_1045
Pf6N2E2_1046 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,220,141 to 1,220,962
_1046
Pf6N2E2_1047 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,220,988 to 1,221,992
_1047
Position (kb)
1220
1221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1219.179 kb on + strand at 1219.329 kb on - strand at 1219.514 kb on + strand at 1219.515 kb on - strand at 1219.515 kb on - strand at 1219.515 kb on - strand at 1219.562 kb on - strand at 1219.572 kb on + strand at 1219.573 kb on - strand at 1219.587 kb on - strand at 1219.640 kb on + strand at 1219.641 kb on - strand at 1219.641 kb on - strand at 1219.658 kb on + strand at 1219.715 kb on + strand at 1219.782 kb on + strand at 1219.783 kb on - strand at 1219.783 kb on - strand at 1219.789 kb on + strand at 1219.789 kb on + strand at 1219.790 kb on - strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.820 kb on - strand at 1219.820 kb on - strand at 1219.909 kb on - strand at 1219.968 kb on + strand at 1219.968 kb on + strand at 1220.001 kb on - strand at 1220.067 kb on + strand at 1220.067 kb on + strand at 1220.067 kb on + strand at 1220.067 kb on + strand at 1220.068 kb on - strand at 1220.121 kb on + strand at 1220.122 kb on - strand at 1220.122 kb on - strand at 1220.157 kb on + strand at 1220.211 kb on + strand at 1220.212 kb on - strand at 1220.388 kb on + strand, within Pf6N2E2_1046 at 1220.389 kb on - strand, within Pf6N2E2_1046 at 1220.434 kb on - strand, within Pf6N2E2_1046 at 1220.642 kb on - strand, within Pf6N2E2_1046 at 1220.737 kb on + strand, within Pf6N2E2_1046 at 1220.781 kb on + strand, within Pf6N2E2_1046 at 1220.782 kb on - strand, within Pf6N2E2_1046 at 1220.811 kb on - strand, within Pf6N2E2_1046 at 1221.053 kb on - strand at 1221.101 kb on + strand, within Pf6N2E2_1047 at 1221.208 kb on + strand, within Pf6N2E2_1047 at 1221.209 kb on - strand, within Pf6N2E2_1047 at 1221.209 kb on - strand, within Pf6N2E2_1047 at 1221.300 kb on + strand, within Pf6N2E2_1047 at 1221.300 kb on + strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.367 kb on + strand, within Pf6N2E2_1047 at 1221.367 kb on + strand, within Pf6N2E2_1047 at 1221.368 kb on - strand, within Pf6N2E2_1047 at 1221.368 kb on - strand, within Pf6N2E2_1047 at 1221.368 kb on - strand, within Pf6N2E2_1047 at 1221.733 kb on + strand, within Pf6N2E2_1047
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,219,179 + -0.3 1,219,329 - +0.5 1,219,514 + +0.6 1,219,515 - -0.2 1,219,515 - -0.9 1,219,515 - +0.6 1,219,562 - -0.8 1,219,572 + +0.5 1,219,573 - -0.6 1,219,587 - +0.2 1,219,640 + -0.7 1,219,641 - -1.1 1,219,641 - +1.1 1,219,658 + -0.3 1,219,715 + +0.1 1,219,782 + +0.3 1,219,783 - +0.8 1,219,783 - +2.5 1,219,789 + +0.9 1,219,789 + -1.0 1,219,790 - -0.4 1,219,819 + +0.4 1,219,819 + +1.5 1,219,819 + +0.1 1,219,819 + -0.1 1,219,820 - +1.2 1,219,820 - -0.1 1,219,909 - +0.5 1,219,968 + +0.6 1,219,968 + +0.0 1,220,001 - -0.7 1,220,067 + +0.9 1,220,067 + +1.1 1,220,067 + -1.1 1,220,067 + +0.1 1,220,068 - -0.5 1,220,121 + +0.2 1,220,122 - -2.1 1,220,122 - -1.4 1,220,157 + +0.4 1,220,211 + -0.4 1,220,212 - -0.3 1,220,388 + Pf6N2E2_1046 0.30 -2.8 1,220,389 - Pf6N2E2_1046 0.30 +0.9 1,220,434 - Pf6N2E2_1046 0.36 +0.2 1,220,642 - Pf6N2E2_1046 0.61 -0.7 1,220,737 + Pf6N2E2_1046 0.73 +0.3 1,220,781 + Pf6N2E2_1046 0.78 +0.2 1,220,782 - Pf6N2E2_1046 0.78 +0.1 1,220,811 - Pf6N2E2_1046 0.82 -0.3 1,221,053 - -0.1 1,221,101 + Pf6N2E2_1047 0.11 +0.4 1,221,208 + Pf6N2E2_1047 0.22 -0.4 1,221,209 - Pf6N2E2_1047 0.22 +0.5 1,221,209 - Pf6N2E2_1047 0.22 +0.2 1,221,300 + Pf6N2E2_1047 0.31 -0.2 1,221,300 + Pf6N2E2_1047 0.31 +0.5 1,221,301 - Pf6N2E2_1047 0.31 +1.6 1,221,301 - Pf6N2E2_1047 0.31 +0.2 1,221,301 - Pf6N2E2_1047 0.31 +1.1 1,221,301 - Pf6N2E2_1047 0.31 -0.2 1,221,367 + Pf6N2E2_1047 0.38 -0.2 1,221,367 + Pf6N2E2_1047 0.38 -0.1 1,221,368 - Pf6N2E2_1047 0.38 -0.6 1,221,368 - Pf6N2E2_1047 0.38 +1.1 1,221,368 - Pf6N2E2_1047 0.38 -0.1 1,221,733 + Pf6N2E2_1047 0.74 +1.3
Or see this region's nucleotide sequence