Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1012 and Pf6N2E2_1013 are separated by 410 nucleotides Pf6N2E2_1013 and Pf6N2E2_1014 are separated by 436 nucleotides
Pf6N2E2_1012 - hypothetical protein, at 1,182,300 to 1,182,518
_1012
Pf6N2E2_1013 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,182,929 to 1,183,753
_1013
Pf6N2E2_1014 - Nitrate/nitrite transporter, at 1,184,190 to 1,184,381
_1014
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1182.119 kb on - strand at 1182.339 kb on + strand, within Pf6N2E2_1012 at 1182.386 kb on + strand, within Pf6N2E2_1012 at 1182.386 kb on + strand, within Pf6N2E2_1012 at 1182.442 kb on + strand, within Pf6N2E2_1012 at 1182.442 kb on + strand, within Pf6N2E2_1012 at 1182.498 kb on + strand at 1182.498 kb on + strand at 1182.498 kb on + strand at 1182.500 kb on + strand at 1182.500 kb on + strand at 1182.500 kb on + strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.501 kb on - strand at 1182.504 kb on + strand at 1182.505 kb on - strand at 1182.544 kb on + strand at 1182.709 kb on + strand at 1182.709 kb on + strand at 1182.709 kb on + strand at 1182.713 kb on + strand at 1182.861 kb on + strand at 1182.861 kb on + strand at 1182.861 kb on + strand at 1182.861 kb on + strand at 1182.896 kb on + strand at 1182.897 kb on - strand at 1182.899 kb on + strand at 1183.031 kb on + strand, within Pf6N2E2_1013 at 1183.031 kb on + strand, within Pf6N2E2_1013 at 1183.031 kb on + strand, within Pf6N2E2_1013 at 1183.032 kb on - strand, within Pf6N2E2_1013 at 1183.032 kb on - strand, within Pf6N2E2_1013 at 1183.057 kb on - strand, within Pf6N2E2_1013 at 1183.114 kb on + strand, within Pf6N2E2_1013 at 1183.114 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.144 kb on + strand, within Pf6N2E2_1013 at 1183.145 kb on - strand, within Pf6N2E2_1013 at 1183.145 kb on - strand, within Pf6N2E2_1013 at 1183.152 kb on + strand, within Pf6N2E2_1013 at 1183.152 kb on + strand, within Pf6N2E2_1013 at 1183.153 kb on - strand, within Pf6N2E2_1013 at 1183.245 kb on + strand, within Pf6N2E2_1013 at 1183.245 kb on + strand, within Pf6N2E2_1013 at 1183.297 kb on - strand, within Pf6N2E2_1013 at 1183.439 kb on + strand, within Pf6N2E2_1013 at 1183.625 kb on + strand, within Pf6N2E2_1013 at 1183.625 kb on + strand, within Pf6N2E2_1013 at 1183.625 kb on + strand, within Pf6N2E2_1013 at 1183.626 kb on - strand, within Pf6N2E2_1013 at 1183.634 kb on + strand, within Pf6N2E2_1013 at 1183.635 kb on - strand, within Pf6N2E2_1013 at 1183.752 kb on - strand at 1183.756 kb on - strand at 1183.756 kb on - strand at 1183.756 kb on - strand at 1183.796 kb on + strand at 1183.833 kb on + strand at 1183.837 kb on + strand at 1183.838 kb on - strand at 1183.868 kb on + strand at 1183.908 kb on - strand at 1183.996 kb on + strand at 1183.996 kb on + strand at 1183.997 kb on - strand at 1184.195 kb on + strand at 1184.254 kb on - strand, within Pf6N2E2_1014 at 1184.254 kb on - strand, within Pf6N2E2_1014 at 1184.280 kb on - strand, within Pf6N2E2_1014 at 1184.600 kb on - strand at 1184.600 kb on - strand at 1184.611 kb on + strand at 1184.622 kb on + strand at 1184.700 kb on - strand at 1184.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,182,119 - +1.0 1,182,339 + Pf6N2E2_1012 0.18 +0.3 1,182,386 + Pf6N2E2_1012 0.39 -2.3 1,182,386 + Pf6N2E2_1012 0.39 +0.2 1,182,442 + Pf6N2E2_1012 0.65 -0.6 1,182,442 + Pf6N2E2_1012 0.65 -0.1 1,182,498 + +0.1 1,182,498 + -0.1 1,182,498 + +0.2 1,182,500 + -0.8 1,182,500 + +0.9 1,182,500 + +0.6 1,182,501 - +2.3 1,182,501 - +0.9 1,182,501 - -0.1 1,182,501 - +0.5 1,182,501 - +0.2 1,182,504 + -0.2 1,182,505 - +1.2 1,182,544 + -0.6 1,182,709 + +0.9 1,182,709 + -0.2 1,182,709 + +0.7 1,182,713 + +0.6 1,182,861 + +0.3 1,182,861 + +0.3 1,182,861 + -0.6 1,182,861 + +0.2 1,182,896 + +0.6 1,182,897 - +0.3 1,182,899 + -0.3 1,183,031 + Pf6N2E2_1013 0.12 +0.4 1,183,031 + Pf6N2E2_1013 0.12 +1.4 1,183,031 + Pf6N2E2_1013 0.12 -0.9 1,183,032 - Pf6N2E2_1013 0.12 -0.1 1,183,032 - Pf6N2E2_1013 0.12 -0.1 1,183,057 - Pf6N2E2_1013 0.16 +0.1 1,183,114 + Pf6N2E2_1013 0.22 -0.7 1,183,114 + Pf6N2E2_1013 0.22 +0.2 1,183,144 + Pf6N2E2_1013 0.26 -2.1 1,183,144 + Pf6N2E2_1013 0.26 -0.5 1,183,144 + Pf6N2E2_1013 0.26 +1.2 1,183,144 + Pf6N2E2_1013 0.26 +0.5 1,183,144 + Pf6N2E2_1013 0.26 -0.3 1,183,144 + Pf6N2E2_1013 0.26 -0.2 1,183,144 + Pf6N2E2_1013 0.26 -0.0 1,183,144 + Pf6N2E2_1013 0.26 -0.7 1,183,144 + Pf6N2E2_1013 0.26 -0.2 1,183,145 - Pf6N2E2_1013 0.26 +0.7 1,183,145 - Pf6N2E2_1013 0.26 -0.1 1,183,152 + Pf6N2E2_1013 0.27 -2.0 1,183,152 + Pf6N2E2_1013 0.27 -0.3 1,183,153 - Pf6N2E2_1013 0.27 -0.2 1,183,245 + Pf6N2E2_1013 0.38 +0.3 1,183,245 + Pf6N2E2_1013 0.38 +0.2 1,183,297 - Pf6N2E2_1013 0.45 +0.5 1,183,439 + Pf6N2E2_1013 0.62 +0.6 1,183,625 + Pf6N2E2_1013 0.84 -1.4 1,183,625 + Pf6N2E2_1013 0.84 +0.1 1,183,625 + Pf6N2E2_1013 0.84 -0.7 1,183,626 - Pf6N2E2_1013 0.84 +0.4 1,183,634 + Pf6N2E2_1013 0.85 +1.1 1,183,635 - Pf6N2E2_1013 0.86 +0.2 1,183,752 - +0.4 1,183,756 - +1.0 1,183,756 - -0.1 1,183,756 - +0.4 1,183,796 + +0.5 1,183,833 + +0.9 1,183,837 + +0.2 1,183,838 - -1.5 1,183,868 + +1.2 1,183,908 - +0.0 1,183,996 + +0.3 1,183,996 + -0.6 1,183,997 - +1.5 1,184,195 + +1.1 1,184,254 - Pf6N2E2_1014 0.33 -0.2 1,184,254 - Pf6N2E2_1014 0.33 +0.2 1,184,280 - Pf6N2E2_1014 0.47 -0.1 1,184,600 - -1.3 1,184,600 - +1.6 1,184,611 + -0.3 1,184,622 + +0.1 1,184,700 - +0.5 1,184,709 + +0.4
Or see this region's nucleotide sequence