Experiment: R2A with Aluminum chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21405 and CA265_RS21410 are separated by 105 nucleotides CA265_RS21410 and CA265_RS21415 are separated by 239 nucleotides CA265_RS21415 and CA265_RS21420 overlap by 1 nucleotides CA265_RS21420 and CA265_RS21425 are separated by 50 nucleotides
CA265_RS21405: CA265_RS21405 - 4,5-DOPA dioxygenase extradiol, at 5,278,761 to 5,279,585
_RS21405
CA265_RS21410: CA265_RS21410 - hypothetical protein, at 5,279,691 to 5,280,263
_RS21410
CA265_RS21415: CA265_RS21415 - AraC family transcriptional regulator, at 5,280,503 to 5,281,063
_RS21415
CA265_RS21420: CA265_RS21420 - hypothetical protein, at 5,281,063 to 5,281,602
_RS21420
CA265_RS21425: CA265_RS21425 - hypothetical protein, at 5,281,653 to 5,282,465
_RS21425
Position (kb)
5280
5281
5282 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5279.530 kb on - strand at 5279.530 kb on - strand at 5279.602 kb on + strand at 5279.617 kb on - strand at 5279.692 kb on + strand at 5279.738 kb on + strand at 5279.739 kb on - strand at 5279.742 kb on + strand at 5279.742 kb on + strand at 5279.772 kb on - strand, within CA265_RS21410 at 5279.772 kb on - strand, within CA265_RS21410 at 5279.774 kb on + strand, within CA265_RS21410 at 5279.774 kb on + strand, within CA265_RS21410 at 5279.776 kb on + strand, within CA265_RS21410 at 5279.777 kb on - strand, within CA265_RS21410 at 5279.822 kb on + strand, within CA265_RS21410 at 5279.823 kb on - strand, within CA265_RS21410 at 5279.826 kb on - strand, within CA265_RS21410 at 5279.841 kb on - strand, within CA265_RS21410 at 5279.917 kb on + strand, within CA265_RS21410 at 5279.979 kb on + strand, within CA265_RS21410 at 5279.979 kb on + strand, within CA265_RS21410 at 5279.980 kb on - strand, within CA265_RS21410 at 5280.035 kb on + strand, within CA265_RS21410 at 5280.045 kb on + strand, within CA265_RS21410 at 5280.046 kb on - strand, within CA265_RS21410 at 5280.048 kb on - strand, within CA265_RS21410 at 5280.068 kb on + strand, within CA265_RS21410 at 5280.068 kb on + strand, within CA265_RS21410 at 5280.069 kb on - strand, within CA265_RS21410 at 5280.246 kb on + strand at 5280.282 kb on + strand at 5280.345 kb on - strand at 5280.348 kb on + strand at 5280.474 kb on + strand at 5280.504 kb on + strand at 5280.506 kb on + strand at 5280.536 kb on - strand at 5280.547 kb on + strand at 5280.643 kb on + strand, within CA265_RS21415 at 5280.655 kb on - strand, within CA265_RS21415 at 5280.753 kb on + strand, within CA265_RS21415 at 5280.754 kb on - strand, within CA265_RS21415 at 5280.754 kb on - strand, within CA265_RS21415 at 5280.754 kb on - strand, within CA265_RS21415 at 5280.832 kb on + strand, within CA265_RS21415 at 5280.833 kb on - strand, within CA265_RS21415 at 5280.914 kb on + strand, within CA265_RS21415 at 5280.978 kb on - strand, within CA265_RS21415 at 5281.037 kb on + strand at 5281.064 kb on + strand at 5281.110 kb on + strand at 5281.111 kb on - strand at 5281.118 kb on + strand, within CA265_RS21420 at 5281.118 kb on + strand, within CA265_RS21420 at 5281.228 kb on - strand, within CA265_RS21420 at 5281.239 kb on - strand, within CA265_RS21420 at 5281.273 kb on - strand, within CA265_RS21420 at 5281.276 kb on - strand, within CA265_RS21420 at 5281.332 kb on + strand, within CA265_RS21420 at 5281.337 kb on - strand at 5281.381 kb on + strand, within CA265_RS21420 at 5281.457 kb on + strand, within CA265_RS21420 at 5281.457 kb on + strand, within CA265_RS21420 at 5281.458 kb on - strand, within CA265_RS21420 at 5281.458 kb on - strand, within CA265_RS21420 at 5281.481 kb on - strand, within CA265_RS21420 at 5281.547 kb on - strand, within CA265_RS21420 at 5281.622 kb on - strand at 5281.696 kb on + strand at 5281.725 kb on - strand at 5281.748 kb on - strand, within CA265_RS21425 at 5281.769 kb on + strand, within CA265_RS21425 at 5281.845 kb on + strand, within CA265_RS21425 at 5281.846 kb on - strand, within CA265_RS21425 at 5281.851 kb on + strand, within CA265_RS21425 at 5281.852 kb on - strand, within CA265_RS21425 at 5281.855 kb on + strand, within CA265_RS21425 at 5281.857 kb on + strand, within CA265_RS21425 at 5281.857 kb on + strand, within CA265_RS21425 at 5281.858 kb on - strand, within CA265_RS21425 at 5281.877 kb on + strand, within CA265_RS21425 at 5281.894 kb on - strand, within CA265_RS21425 at 5281.955 kb on + strand, within CA265_RS21425 at 5281.980 kb on - strand, within CA265_RS21425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Aluminum chloride 1 mM remove 5,279,530 - +0.8 5,279,530 - +0.1 5,279,602 + -0.4 5,279,617 - -1.3 5,279,692 + -0.1 5,279,738 + +0.1 5,279,739 - +0.0 5,279,742 + +0.4 5,279,742 + +1.1 5,279,772 - CA265_RS21410 0.14 +0.8 5,279,772 - CA265_RS21410 0.14 -0.2 5,279,774 + CA265_RS21410 0.14 -0.1 5,279,774 + CA265_RS21410 0.14 -0.1 5,279,776 + CA265_RS21410 0.15 +0.3 5,279,777 - CA265_RS21410 0.15 -1.2 5,279,822 + CA265_RS21410 0.23 -0.1 5,279,823 - CA265_RS21410 0.23 -1.5 5,279,826 - CA265_RS21410 0.24 -1.4 5,279,841 - CA265_RS21410 0.26 +1.2 5,279,917 + CA265_RS21410 0.39 +0.6 5,279,979 + CA265_RS21410 0.50 -0.3 5,279,979 + CA265_RS21410 0.50 -0.3 5,279,980 - CA265_RS21410 0.50 +1.0 5,280,035 + CA265_RS21410 0.60 -0.9 5,280,045 + CA265_RS21410 0.62 +0.9 5,280,046 - CA265_RS21410 0.62 +1.2 5,280,048 - CA265_RS21410 0.62 +0.3 5,280,068 + CA265_RS21410 0.66 -0.5 5,280,068 + CA265_RS21410 0.66 +0.1 5,280,069 - CA265_RS21410 0.66 +0.6 5,280,246 + -0.4 5,280,282 + +0.5 5,280,345 - -0.7 5,280,348 + -0.2 5,280,474 + -0.2 5,280,504 + -0.1 5,280,506 + +0.7 5,280,536 - +0.7 5,280,547 + -0.2 5,280,643 + CA265_RS21415 0.25 +0.6 5,280,655 - CA265_RS21415 0.27 +0.8 5,280,753 + CA265_RS21415 0.45 +0.2 5,280,754 - CA265_RS21415 0.45 -0.7 5,280,754 - CA265_RS21415 0.45 -0.3 5,280,754 - CA265_RS21415 0.45 -1.0 5,280,832 + CA265_RS21415 0.59 +0.2 5,280,833 - CA265_RS21415 0.59 +0.6 5,280,914 + CA265_RS21415 0.73 -0.1 5,280,978 - CA265_RS21415 0.85 -0.9 5,281,037 + -0.4 5,281,064 + +0.2 5,281,110 + -0.1 5,281,111 - -0.0 5,281,118 + CA265_RS21420 0.10 +1.4 5,281,118 + CA265_RS21420 0.10 +1.3 5,281,228 - CA265_RS21420 0.31 +0.5 5,281,239 - CA265_RS21420 0.33 +0.6 5,281,273 - CA265_RS21420 0.39 +0.6 5,281,276 - CA265_RS21420 0.39 +0.6 5,281,332 + CA265_RS21420 0.50 +0.2 5,281,337 - -0.2 5,281,381 + CA265_RS21420 0.59 -0.8 5,281,457 + CA265_RS21420 0.73 -0.0 5,281,457 + CA265_RS21420 0.73 +0.1 5,281,458 - CA265_RS21420 0.73 +0.3 5,281,458 - CA265_RS21420 0.73 -0.9 5,281,481 - CA265_RS21420 0.77 -1.0 5,281,547 - CA265_RS21420 0.90 -0.1 5,281,622 - -0.6 5,281,696 + +1.0 5,281,725 - -1.2 5,281,748 - CA265_RS21425 0.12 -2.3 5,281,769 + CA265_RS21425 0.14 +0.1 5,281,845 + CA265_RS21425 0.24 -1.7 5,281,846 - CA265_RS21425 0.24 +0.4 5,281,851 + CA265_RS21425 0.24 +0.4 5,281,852 - CA265_RS21425 0.24 -0.8 5,281,855 + CA265_RS21425 0.25 -0.6 5,281,857 + CA265_RS21425 0.25 +0.2 5,281,857 + CA265_RS21425 0.25 -0.7 5,281,858 - CA265_RS21425 0.25 -2.9 5,281,877 + CA265_RS21425 0.28 +0.0 5,281,894 - CA265_RS21425 0.30 -0.4 5,281,955 + CA265_RS21425 0.37 +0.4 5,281,980 - CA265_RS21425 0.40 +0.2
Or see this region's nucleotide sequence