Experiment: R2A with Aluminum chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03645 and CA265_RS03650 are separated by 70 nucleotides CA265_RS03650 and CA265_RS03655 are separated by 68 nucleotides CA265_RS03655 and CA265_RS03660 are separated by 29 nucleotides
CA265_RS03645: CA265_RS03645 - L,D-transpeptidase, at 870,738 to 872,300
_RS03645
CA265_RS03650: CA265_RS03650 - NUDIX hydrolase, at 872,371 to 873,063
_RS03650
CA265_RS03655: CA265_RS03655 - phosphoribosylpyrophosphate synthetase, at 873,132 to 873,965
_RS03655
CA265_RS03660: CA265_RS03660 - hypothetical protein, at 873,995 to 875,047
_RS03660
Position (kb) 872
873
874 Strain fitness (log2 ratio) -2
-1
0
1 at 871.379 kb on - strand, within CA265_RS03645 at 871.464 kb on + strand, within CA265_RS03645 at 871.464 kb on + strand, within CA265_RS03645 at 871.465 kb on - strand, within CA265_RS03645 at 871.465 kb on - strand, within CA265_RS03645 at 871.522 kb on + strand, within CA265_RS03645 at 871.527 kb on - strand, within CA265_RS03645 at 871.575 kb on - strand, within CA265_RS03645 at 871.741 kb on - strand, within CA265_RS03645 at 871.744 kb on - strand, within CA265_RS03645 at 871.744 kb on - strand, within CA265_RS03645 at 871.833 kb on - strand, within CA265_RS03645 at 871.920 kb on + strand, within CA265_RS03645 at 871.937 kb on + strand, within CA265_RS03645 at 871.992 kb on - strand, within CA265_RS03645 at 872.043 kb on + strand, within CA265_RS03645 at 872.044 kb on - strand, within CA265_RS03645 at 872.085 kb on - strand, within CA265_RS03645 at 872.085 kb on - strand, within CA265_RS03645 at 872.087 kb on + strand, within CA265_RS03645 at 872.088 kb on - strand, within CA265_RS03645 at 872.096 kb on - strand, within CA265_RS03645 at 872.139 kb on - strand, within CA265_RS03645 at 872.202 kb on + strand at 872.203 kb on - strand at 872.203 kb on - strand at 872.356 kb on + strand at 872.356 kb on + strand at 872.441 kb on + strand, within CA265_RS03650 at 872.472 kb on - strand, within CA265_RS03650 at 872.490 kb on - strand, within CA265_RS03650 at 872.534 kb on + strand, within CA265_RS03650 at 872.607 kb on + strand, within CA265_RS03650 at 872.608 kb on - strand, within CA265_RS03650 at 872.648 kb on - strand, within CA265_RS03650 at 872.660 kb on + strand, within CA265_RS03650 at 872.754 kb on + strand, within CA265_RS03650 at 872.826 kb on - strand, within CA265_RS03650 at 872.867 kb on - strand, within CA265_RS03650 at 872.950 kb on - strand, within CA265_RS03650 at 873.013 kb on + strand at 873.137 kb on - strand at 873.222 kb on - strand, within CA265_RS03655 at 873.261 kb on - strand, within CA265_RS03655 at 873.309 kb on - strand, within CA265_RS03655 at 873.311 kb on + strand, within CA265_RS03655 at 873.312 kb on - strand, within CA265_RS03655 at 873.312 kb on - strand, within CA265_RS03655 at 873.359 kb on + strand, within CA265_RS03655 at 873.366 kb on + strand, within CA265_RS03655 at 873.438 kb on - strand, within CA265_RS03655 at 873.441 kb on - strand, within CA265_RS03655 at 873.522 kb on - strand, within CA265_RS03655 at 873.553 kb on + strand, within CA265_RS03655 at 873.584 kb on + strand, within CA265_RS03655 at 873.584 kb on + strand, within CA265_RS03655 at 873.587 kb on - strand, within CA265_RS03655 at 873.656 kb on - strand, within CA265_RS03655 at 873.656 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.749 kb on - strand, within CA265_RS03655 at 873.755 kb on + strand, within CA265_RS03655 at 873.826 kb on - strand, within CA265_RS03655 at 873.829 kb on + strand, within CA265_RS03655 at 873.829 kb on + strand, within CA265_RS03655 at 873.830 kb on - strand, within CA265_RS03655 at 873.899 kb on + strand at 873.943 kb on + strand at 873.969 kb on + strand at 873.974 kb on - strand at 873.982 kb on + strand at 873.983 kb on - strand at 874.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Aluminum chloride 1 mM remove 871,379 - CA265_RS03645 0.41 -0.8 871,464 + CA265_RS03645 0.46 +0.0 871,464 + CA265_RS03645 0.46 -1.1 871,465 - CA265_RS03645 0.47 -0.5 871,465 - CA265_RS03645 0.47 -0.5 871,522 + CA265_RS03645 0.50 -1.0 871,527 - CA265_RS03645 0.50 -0.4 871,575 - CA265_RS03645 0.54 -0.1 871,741 - CA265_RS03645 0.64 -0.8 871,744 - CA265_RS03645 0.64 +0.2 871,744 - CA265_RS03645 0.64 -0.5 871,833 - CA265_RS03645 0.70 -0.3 871,920 + CA265_RS03645 0.76 -0.1 871,937 + CA265_RS03645 0.77 +0.5 871,992 - CA265_RS03645 0.80 +0.2 872,043 + CA265_RS03645 0.83 -0.4 872,044 - CA265_RS03645 0.84 -0.2 872,085 - CA265_RS03645 0.86 +0.8 872,085 - CA265_RS03645 0.86 -0.8 872,087 + CA265_RS03645 0.86 -0.6 872,088 - CA265_RS03645 0.86 -0.9 872,096 - CA265_RS03645 0.87 -0.2 872,139 - CA265_RS03645 0.90 -0.7 872,202 + -1.7 872,203 - -0.4 872,203 - -0.4 872,356 + +0.3 872,356 + -0.7 872,441 + CA265_RS03650 0.10 -1.7 872,472 - CA265_RS03650 0.15 -1.3 872,490 - CA265_RS03650 0.17 -0.4 872,534 + CA265_RS03650 0.24 -0.0 872,607 + CA265_RS03650 0.34 -0.7 872,608 - CA265_RS03650 0.34 -1.9 872,648 - CA265_RS03650 0.40 -0.2 872,660 + CA265_RS03650 0.42 -0.2 872,754 + CA265_RS03650 0.55 -1.7 872,826 - CA265_RS03650 0.66 -1.1 872,867 - CA265_RS03650 0.72 -0.1 872,950 - CA265_RS03650 0.84 -1.7 873,013 + -0.9 873,137 - +0.7 873,222 - CA265_RS03655 0.11 -0.5 873,261 - CA265_RS03655 0.15 +0.7 873,309 - CA265_RS03655 0.21 -0.4 873,311 + CA265_RS03655 0.21 -1.1 873,312 - CA265_RS03655 0.22 -0.2 873,312 - CA265_RS03655 0.22 +1.3 873,359 + CA265_RS03655 0.27 -0.4 873,366 + CA265_RS03655 0.28 -0.6 873,438 - CA265_RS03655 0.37 -0.1 873,441 - CA265_RS03655 0.37 +0.8 873,522 - CA265_RS03655 0.47 +0.5 873,553 + CA265_RS03655 0.50 -0.8 873,584 + CA265_RS03655 0.54 -0.2 873,584 + CA265_RS03655 0.54 +0.1 873,587 - CA265_RS03655 0.55 +0.3 873,656 - CA265_RS03655 0.63 -0.7 873,656 - CA265_RS03655 0.63 -0.1 873,710 - CA265_RS03655 0.69 -0.0 873,710 - CA265_RS03655 0.69 -0.0 873,710 - CA265_RS03655 0.69 -1.1 873,710 - CA265_RS03655 0.69 +0.5 873,749 - CA265_RS03655 0.74 +1.6 873,755 + CA265_RS03655 0.75 -0.6 873,826 - CA265_RS03655 0.83 +0.3 873,829 + CA265_RS03655 0.84 -2.6 873,829 + CA265_RS03655 0.84 -0.7 873,830 - CA265_RS03655 0.84 +0.2 873,899 + -0.7 873,943 + -0.2 873,969 + -1.1 873,974 - +0.0 873,982 + -0.1 873,983 - -0.2 874,033 - +0.3
Or see this region's nucleotide sequence