Experiment: R2A with Aluminum chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00585 and CA265_RS00590 are separated by 74 nucleotides CA265_RS00590 and CA265_RS00595 are separated by 223 nucleotides CA265_RS00595 and CA265_RS00600 are separated by 114 nucleotides
CA265_RS00585: CA265_RS00585 - translocation/assembly module TamB, at 130,429 to 134,859
_RS00585
CA265_RS00590: CA265_RS00590 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 134,934 to 135,935
_RS00590
CA265_RS00595: CA265_RS00595 - hypothetical protein, at 136,159 to 136,356
_RS00595
CA265_RS00600: CA265_RS00600 - recombinase RecA, at 136,471 to 137,484
_RS00600
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 133.938 kb on + strand, within CA265_RS00585 at 133.963 kb on + strand, within CA265_RS00585 at 133.983 kb on + strand, within CA265_RS00585 at 133.984 kb on - strand, within CA265_RS00585 at 134.118 kb on + strand, within CA265_RS00585 at 134.118 kb on + strand, within CA265_RS00585 at 134.119 kb on - strand, within CA265_RS00585 at 134.122 kb on + strand, within CA265_RS00585 at 134.122 kb on + strand, within CA265_RS00585 at 134.122 kb on + strand, within CA265_RS00585 at 134.127 kb on - strand, within CA265_RS00585 at 134.127 kb on - strand, within CA265_RS00585 at 134.169 kb on - strand, within CA265_RS00585 at 134.233 kb on + strand, within CA265_RS00585 at 134.373 kb on + strand, within CA265_RS00585 at 134.374 kb on - strand, within CA265_RS00585 at 134.418 kb on - strand at 134.442 kb on - strand at 134.463 kb on + strand at 134.474 kb on + strand at 134.475 kb on - strand at 134.475 kb on - strand at 134.497 kb on + strand at 134.508 kb on + strand at 134.523 kb on - strand at 134.528 kb on - strand at 134.553 kb on + strand at 134.623 kb on - strand at 134.641 kb on - strand at 134.702 kb on - strand at 134.734 kb on - strand at 134.767 kb on - strand at 134.783 kb on + strand at 134.783 kb on + strand at 134.783 kb on + strand at 134.784 kb on - strand at 134.864 kb on - strand at 135.934 kb on - strand at 136.148 kb on + strand at 136.148 kb on + strand at 136.158 kb on + strand at 136.158 kb on + strand at 136.158 kb on + strand at 136.159 kb on - strand at 136.308 kb on + strand, within CA265_RS00595 at 136.309 kb on - strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.310 kb on + strand, within CA265_RS00595 at 136.311 kb on - strand, within CA265_RS00595 at 136.384 kb on - strand at 136.402 kb on + strand at 136.403 kb on - strand at 136.427 kb on + strand at 136.433 kb on + strand at 136.450 kb on - strand at 136.454 kb on + strand at 136.498 kb on + strand at 136.575 kb on + strand, within CA265_RS00600 at 136.594 kb on - strand, within CA265_RS00600 at 136.632 kb on + strand, within CA265_RS00600 at 136.806 kb on + strand, within CA265_RS00600 at 136.810 kb on + strand, within CA265_RS00600 at 136.895 kb on - strand, within CA265_RS00600 at 136.911 kb on + strand, within CA265_RS00600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Aluminum chloride 1 mM remove 133,938 + CA265_RS00585 0.79 -4.1 133,963 + CA265_RS00585 0.80 -1.3 133,983 + CA265_RS00585 0.80 -1.7 133,984 - CA265_RS00585 0.80 -2.3 134,118 + CA265_RS00585 0.83 -3.1 134,118 + CA265_RS00585 0.83 -2.8 134,119 - CA265_RS00585 0.83 -3.3 134,122 + CA265_RS00585 0.83 -2.2 134,122 + CA265_RS00585 0.83 -3.6 134,122 + CA265_RS00585 0.83 -4.3 134,127 - CA265_RS00585 0.83 -4.1 134,127 - CA265_RS00585 0.83 -3.5 134,169 - CA265_RS00585 0.84 -0.9 134,233 + CA265_RS00585 0.86 -3.7 134,373 + CA265_RS00585 0.89 -3.3 134,374 - CA265_RS00585 0.89 -2.3 134,418 - -2.9 134,442 - -1.6 134,463 + -2.4 134,474 + -4.2 134,475 - -3.1 134,475 - -3.2 134,497 + -2.7 134,508 + -1.9 134,523 - -4.5 134,528 - -0.1 134,553 + -3.3 134,623 - -0.1 134,641 - -3.0 134,702 - -1.9 134,734 - -3.1 134,767 - -3.4 134,783 + -2.9 134,783 + -3.2 134,783 + -1.3 134,784 - -4.0 134,864 - -2.9 135,934 - -0.2 136,148 + -1.1 136,148 + -0.8 136,158 + -1.4 136,158 + -0.6 136,158 + +0.5 136,159 - -0.2 136,308 + CA265_RS00595 0.75 +1.9 136,309 - CA265_RS00595 0.76 +0.2 136,310 + CA265_RS00595 0.76 +0.4 136,310 + CA265_RS00595 0.76 -1.2 136,310 + CA265_RS00595 0.76 +0.2 136,311 - CA265_RS00595 0.77 +0.3 136,384 - +0.8 136,402 + -1.6 136,403 - +0.3 136,427 + -0.1 136,433 + -1.1 136,450 - -0.1 136,454 + +0.3 136,498 + +0.3 136,575 + CA265_RS00600 0.10 -1.5 136,594 - CA265_RS00600 0.12 +0.5 136,632 + CA265_RS00600 0.16 -0.6 136,806 + CA265_RS00600 0.33 +0.2 136,810 + CA265_RS00600 0.33 -1.6 136,895 - CA265_RS00600 0.42 +0.2 136,911 + CA265_RS00600 0.43 +0.1
Or see this region's nucleotide sequence