Experiment: CDM1 without Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19420 and BBR_RS19425 are separated by 32 nucleotides BBR_RS19425 and BBR_RS19430 are separated by 39 nucleotides BBR_RS19430 and BBR_RS19435 are separated by 214 nucleotides
BBR_RS19420: BBR_RS19420 - transcription termination/antitermination factor NusG, at 2,140,906 to 2,141,733
_RS19420
BBR_RS19425: BBR_RS19425 - preprotein translocase subunit SecE, at 2,141,766 to 2,141,993
_RS19425
BBR_RS19430: BBR_RS19430 - tRNA-Trp, at 2,142,033 to 2,142,108
_RS19430
BBR_RS19435: BBR_RS19435 - pyridoxal phosphate-dependent aminotransferase, at 2,142,323 to 2,143,528
_RS19435
Position (kb)
2141
2142 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2140.779 kb on - strand at 2140.780 kb on + strand at 2140.788 kb on - strand at 2140.788 kb on - strand at 2140.804 kb on + strand at 2140.804 kb on + strand at 2140.812 kb on - strand at 2140.848 kb on + strand at 2142.209 kb on - strand at 2142.264 kb on - strand at 2142.268 kb on - strand at 2142.280 kb on + strand at 2142.280 kb on + strand at 2142.283 kb on - strand at 2142.283 kb on - strand at 2142.298 kb on + strand at 2142.298 kb on + strand at 2142.299 kb on - strand at 2142.304 kb on - strand at 2142.304 kb on - strand at 2142.304 kb on - strand at 2142.316 kb on - strand at 2142.322 kb on - strand at 2142.345 kb on - strand at 2142.353 kb on - strand at 2142.746 kb on + strand, within BBR_RS19435 at 2142.746 kb on + strand, within BBR_RS19435 at 2142.829 kb on - strand, within BBR_RS19435 at 2142.887 kb on + strand, within BBR_RS19435 at 2142.895 kb on - strand, within BBR_RS19435
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Phenylalanine remove 2,140,779 - -1.1 2,140,780 + -0.5 2,140,788 - -1.9 2,140,788 - +0.0 2,140,804 + -0.5 2,140,804 + -1.0 2,140,812 - -0.3 2,140,848 + -0.8 2,142,209 - +0.8 2,142,264 - -0.2 2,142,268 - +2.6 2,142,280 + -1.4 2,142,280 + +0.6 2,142,283 - +1.8 2,142,283 - +1.1 2,142,298 + -1.9 2,142,298 + -1.0 2,142,299 - +0.7 2,142,304 - +2.2 2,142,304 - -2.5 2,142,304 - -2.0 2,142,316 - -2.0 2,142,322 - +2.1 2,142,345 - +1.6 2,142,353 - +0.7 2,142,746 + BBR_RS19435 0.35 +1.6 2,142,746 + BBR_RS19435 0.35 -0.1 2,142,829 - BBR_RS19435 0.42 +5.6 2,142,887 + BBR_RS19435 0.47 +2.8 2,142,895 - BBR_RS19435 0.47 +1.2
Or see this region's nucleotide sequence