Experiment: CDM1 without Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15560 and BBR_RS15565 are separated by 500 nucleotides BBR_RS15565 and BBR_RS15570 are separated by 172 nucleotides
BBR_RS15560: BBR_RS15560 - aminotransferase class V-fold PLP-dependent enzyme, at 1,263,901 to 1,265,397
_RS15560
BBR_RS15565: BBR_RS15565 - hypothetical protein, at 1,265,898 to 1,267,082
_RS15565
BBR_RS15570: BBR_RS15570 - branched-chain amino acid transport system II carrier protein, at 1,267,255 to 1,268,592
_RS15570
Position (kb)
1265
1266
1267
1268 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1264.965 kb on + strand, within BBR_RS15560 at 1265.009 kb on + strand, within BBR_RS15560 at 1265.009 kb on + strand, within BBR_RS15560 at 1265.055 kb on + strand, within BBR_RS15560 at 1265.072 kb on + strand, within BBR_RS15560 at 1265.078 kb on - strand, within BBR_RS15560 at 1265.123 kb on - strand, within BBR_RS15560 at 1265.340 kb on + strand at 1265.340 kb on + strand at 1265.431 kb on + strand at 1265.473 kb on + strand at 1265.473 kb on + strand at 1265.531 kb on - strand at 1265.534 kb on + strand at 1265.546 kb on + strand at 1265.659 kb on - strand at 1265.807 kb on - strand at 1265.812 kb on + strand at 1265.815 kb on - strand at 1265.821 kb on + strand at 1265.896 kb on + strand at 1265.896 kb on + strand at 1265.927 kb on - strand at 1265.927 kb on - strand at 1265.939 kb on + strand at 1265.944 kb on + strand at 1265.944 kb on + strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.971 kb on + strand at 1266.024 kb on + strand, within BBR_RS15565 at 1266.043 kb on - strand, within BBR_RS15565 at 1266.043 kb on - strand, within BBR_RS15565 at 1266.110 kb on + strand, within BBR_RS15565 at 1266.110 kb on + strand, within BBR_RS15565 at 1266.145 kb on - strand, within BBR_RS15565 at 1266.145 kb on - strand, within BBR_RS15565 at 1266.178 kb on + strand, within BBR_RS15565 at 1266.178 kb on + strand, within BBR_RS15565 at 1266.231 kb on - strand, within BBR_RS15565 at 1266.291 kb on - strand, within BBR_RS15565 at 1266.338 kb on - strand, within BBR_RS15565 at 1266.376 kb on + strand, within BBR_RS15565 at 1266.570 kb on + strand, within BBR_RS15565 at 1266.580 kb on + strand, within BBR_RS15565 at 1266.588 kb on - strand, within BBR_RS15565 at 1266.607 kb on - strand, within BBR_RS15565 at 1266.646 kb on - strand, within BBR_RS15565 at 1266.771 kb on + strand, within BBR_RS15565 at 1266.779 kb on - strand, within BBR_RS15565 at 1266.779 kb on - strand, within BBR_RS15565 at 1266.811 kb on - strand, within BBR_RS15565 at 1266.848 kb on - strand, within BBR_RS15565 at 1266.911 kb on + strand, within BBR_RS15565 at 1267.007 kb on + strand at 1267.151 kb on + strand at 1267.151 kb on + strand at 1267.151 kb on - strand at 1267.182 kb on + strand at 1267.245 kb on + strand at 1267.245 kb on + strand at 1267.332 kb on - strand at 1267.348 kb on - strand at 1267.358 kb on - strand at 1267.442 kb on + strand, within BBR_RS15570 at 1267.442 kb on + strand, within BBR_RS15570 at 1267.442 kb on + strand, within BBR_RS15570 at 1267.450 kb on - strand, within BBR_RS15570 at 1267.479 kb on + strand, within BBR_RS15570 at 1267.479 kb on + strand, within BBR_RS15570 at 1267.487 kb on - strand, within BBR_RS15570 at 1267.504 kb on - strand, within BBR_RS15570 at 1267.599 kb on + strand, within BBR_RS15570 at 1267.601 kb on + strand, within BBR_RS15570 at 1267.630 kb on - strand, within BBR_RS15570 at 1267.690 kb on - strand, within BBR_RS15570 at 1267.803 kb on + strand, within BBR_RS15570 at 1267.837 kb on - strand, within BBR_RS15570 at 1267.852 kb on + strand, within BBR_RS15570 at 1267.915 kb on + strand, within BBR_RS15570
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Phenylalanine remove 1,264,965 + BBR_RS15560 0.71 -1.6 1,265,009 + BBR_RS15560 0.74 +1.2 1,265,009 + BBR_RS15560 0.74 +2.3 1,265,055 + BBR_RS15560 0.77 +0.3 1,265,072 + BBR_RS15560 0.78 +3.3 1,265,078 - BBR_RS15560 0.79 +0.7 1,265,123 - BBR_RS15560 0.82 +1.6 1,265,340 + -1.0 1,265,340 + +2.1 1,265,431 + +0.0 1,265,473 + -0.4 1,265,473 + +0.7 1,265,531 - -0.8 1,265,534 + +0.8 1,265,546 + -0.7 1,265,659 - +0.8 1,265,807 - -2.1 1,265,812 + +0.2 1,265,815 - +1.0 1,265,821 + -1.6 1,265,896 + +0.7 1,265,896 + +0.3 1,265,927 - +0.7 1,265,927 - -1.3 1,265,939 + -1.5 1,265,944 + +1.2 1,265,944 + +2.0 1,265,955 - +1.3 1,265,955 - -0.8 1,265,955 - -0.0 1,265,955 - -0.1 1,265,971 + +3.2 1,266,024 + BBR_RS15565 0.11 -1.2 1,266,043 - BBR_RS15565 0.12 +0.2 1,266,043 - BBR_RS15565 0.12 -1.8 1,266,110 + BBR_RS15565 0.18 +0.6 1,266,110 + BBR_RS15565 0.18 +3.1 1,266,145 - BBR_RS15565 0.21 +0.1 1,266,145 - BBR_RS15565 0.21 -0.3 1,266,178 + BBR_RS15565 0.24 +1.2 1,266,178 + BBR_RS15565 0.24 -0.5 1,266,231 - BBR_RS15565 0.28 +0.4 1,266,291 - BBR_RS15565 0.33 -2.6 1,266,338 - BBR_RS15565 0.37 +1.1 1,266,376 + BBR_RS15565 0.40 -0.1 1,266,570 + BBR_RS15565 0.57 +1.0 1,266,580 + BBR_RS15565 0.58 -0.6 1,266,588 - BBR_RS15565 0.58 -1.4 1,266,607 - BBR_RS15565 0.60 -0.0 1,266,646 - BBR_RS15565 0.63 +2.6 1,266,771 + BBR_RS15565 0.74 -0.8 1,266,779 - BBR_RS15565 0.74 +1.8 1,266,779 - BBR_RS15565 0.74 -0.3 1,266,811 - BBR_RS15565 0.77 -0.4 1,266,848 - BBR_RS15565 0.80 +1.7 1,266,911 + BBR_RS15565 0.85 +0.1 1,267,007 + +0.7 1,267,151 + -2.7 1,267,151 + +1.4 1,267,151 - +2.6 1,267,182 + +0.8 1,267,245 + -0.5 1,267,245 + -0.7 1,267,332 - -1.8 1,267,348 - +1.5 1,267,358 - -2.0 1,267,442 + BBR_RS15570 0.14 -1.6 1,267,442 + BBR_RS15570 0.14 +1.7 1,267,442 + BBR_RS15570 0.14 -0.7 1,267,450 - BBR_RS15570 0.15 -1.4 1,267,479 + BBR_RS15570 0.17 +2.0 1,267,479 + BBR_RS15570 0.17 -2.0 1,267,487 - BBR_RS15570 0.17 +1.9 1,267,504 - BBR_RS15570 0.19 -1.0 1,267,599 + BBR_RS15570 0.26 -0.3 1,267,601 + BBR_RS15570 0.26 +1.0 1,267,630 - BBR_RS15570 0.28 -2.6 1,267,690 - BBR_RS15570 0.33 +0.4 1,267,803 + BBR_RS15570 0.41 +0.2 1,267,837 - BBR_RS15570 0.43 +0.4 1,267,852 + BBR_RS15570 0.45 +1.4 1,267,915 + BBR_RS15570 0.49 +1.8
Or see this region's nucleotide sequence