Experiment: Paraquat dichloride 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_175 and Ac3H11_176 are separated by 86 nucleotides Ac3H11_176 and Ac3H11_177 are separated by 9 nucleotides Ac3H11_177 and Ac3H11_178 are separated by 91 nucleotides
Ac3H11_175 - Permease of the drug/metabolite transporter (DMT) superfamily, at 63,855 to 64,748
_175
Ac3H11_176 - Transcriptional regulator, MarR family, at 64,835 to 65,380
_176
Ac3H11_177 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 65,390 to 66,163
_177
Ac3H11_178 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 66,255 to 67,439
_178
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 64.672 kb on + strand at 64.672 kb on + strand at 64.672 kb on + strand at 64.672 kb on + strand at 64.672 kb on + strand at 64.672 kb on + strand at 64.673 kb on - strand at 64.788 kb on + strand at 64.788 kb on + strand at 64.789 kb on - strand at 64.789 kb on - strand at 65.052 kb on - strand, within Ac3H11_176 at 65.368 kb on + strand at 65.369 kb on - strand at 65.378 kb on + strand at 65.379 kb on - strand at 65.398 kb on + strand at 65.398 kb on + strand at 65.398 kb on + strand at 65.399 kb on - strand at 65.399 kb on - strand at 65.399 kb on - strand at 65.399 kb on - strand at 65.408 kb on + strand at 65.408 kb on + strand at 65.408 kb on + strand at 65.408 kb on + strand at 65.408 kb on + strand at 65.409 kb on - strand at 65.409 kb on - strand at 65.409 kb on - strand at 65.409 kb on - strand at 65.409 kb on - strand at 65.409 kb on - strand at 65.409 kb on - strand at 65.871 kb on - strand, within Ac3H11_177 at 65.952 kb on + strand, within Ac3H11_177 at 65.952 kb on + strand, within Ac3H11_177 at 65.952 kb on + strand, within Ac3H11_177 at 65.952 kb on + strand, within Ac3H11_177 at 65.952 kb on + strand, within Ac3H11_177 at 65.952 kb on + strand, within Ac3H11_177 at 65.953 kb on - strand, within Ac3H11_177 at 65.953 kb on - strand, within Ac3H11_177 at 65.953 kb on - strand, within Ac3H11_177 at 65.953 kb on - strand, within Ac3H11_177 at 65.953 kb on - strand, within Ac3H11_177 at 65.953 kb on - strand, within Ac3H11_177 at 66.168 kb on - strand at 66.170 kb on + strand at 66.188 kb on + strand at 66.189 kb on - strand at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.450 kb on + strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.451 kb on - strand, within Ac3H11_178 at 66.837 kb on + strand, within Ac3H11_178 at 66.838 kb on - strand, within Ac3H11_178 at 66.894 kb on + strand, within Ac3H11_178 at 66.894 kb on + strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.01 mg/ml remove 64,672 + -1.3 64,672 + -0.3 64,672 + +1.1 64,672 + +1.1 64,672 + +0.7 64,672 + -0.1 64,673 - +0.9 64,788 + +0.3 64,788 + -0.8 64,789 - -2.6 64,789 - -2.0 65,052 - Ac3H11_176 0.40 -0.6 65,368 + -0.6 65,369 - -2.3 65,378 + -0.8 65,379 - -1.6 65,398 + -2.6 65,398 + +1.9 65,398 + +0.6 65,399 - +0.4 65,399 - -1.4 65,399 - -0.4 65,399 - +2.4 65,408 + +1.5 65,408 + -1.1 65,408 + +1.4 65,408 + -0.6 65,408 + -2.0 65,409 - -0.0 65,409 - -1.8 65,409 - -2.6 65,409 - +0.9 65,409 - +1.1 65,409 - +0.2 65,409 - -1.7 65,871 - Ac3H11_177 0.62 -2.3 65,952 + Ac3H11_177 0.73 +2.0 65,952 + Ac3H11_177 0.73 -1.1 65,952 + Ac3H11_177 0.73 -0.6 65,952 + Ac3H11_177 0.73 +1.5 65,952 + Ac3H11_177 0.73 -0.5 65,952 + Ac3H11_177 0.73 +0.4 65,953 - Ac3H11_177 0.73 -2.2 65,953 - Ac3H11_177 0.73 -0.2 65,953 - Ac3H11_177 0.73 -0.8 65,953 - Ac3H11_177 0.73 +1.1 65,953 - Ac3H11_177 0.73 -0.4 65,953 - Ac3H11_177 0.73 -0.8 66,168 - +0.4 66,170 + -1.6 66,188 + +0.7 66,189 - -0.8 66,450 + Ac3H11_178 0.16 -2.2 66,450 + Ac3H11_178 0.16 +0.9 66,450 + Ac3H11_178 0.16 +2.3 66,450 + Ac3H11_178 0.16 -0.1 66,450 + Ac3H11_178 0.16 -3.0 66,450 + Ac3H11_178 0.16 -2.8 66,450 + Ac3H11_178 0.16 -0.7 66,450 + Ac3H11_178 0.16 +1.4 66,450 + Ac3H11_178 0.16 +0.4 66,450 + Ac3H11_178 0.16 +0.6 66,450 + Ac3H11_178 0.16 -0.0 66,450 + Ac3H11_178 0.16 +0.7 66,451 - Ac3H11_178 0.17 +2.0 66,451 - Ac3H11_178 0.17 +2.2 66,451 - Ac3H11_178 0.17 -0.7 66,451 - Ac3H11_178 0.17 +0.4 66,451 - Ac3H11_178 0.17 +0.4 66,451 - Ac3H11_178 0.17 -1.3 66,451 - Ac3H11_178 0.17 -0.8 66,451 - Ac3H11_178 0.17 -3.3 66,451 - Ac3H11_178 0.17 +1.0 66,451 - Ac3H11_178 0.17 -2.1 66,451 - Ac3H11_178 0.17 +0.9 66,451 - Ac3H11_178 0.17 -3.1 66,451 - Ac3H11_178 0.17 +0.4 66,451 - Ac3H11_178 0.17 -0.6 66,451 - Ac3H11_178 0.17 +0.2 66,837 + Ac3H11_178 0.49 -2.3 66,838 - Ac3H11_178 0.49 -0.4 66,894 + Ac3H11_178 0.54 +1.0 66,894 + Ac3H11_178 0.54 +0.4 66,895 - Ac3H11_178 0.54 +1.6 66,895 - Ac3H11_178 0.54 +2.4 66,895 - Ac3H11_178 0.54 +4.5 66,895 - Ac3H11_178 0.54 -2.2 67,119 + Ac3H11_178 0.73 -0.1 67,119 + Ac3H11_178 0.73 +1.1 67,119 + Ac3H11_178 0.73 +0.8 67,119 + Ac3H11_178 0.73 +2.0 67,119 + Ac3H11_178 0.73 +0.5 67,119 + Ac3H11_178 0.73 -0.1 67,119 + Ac3H11_178 0.73 -1.0 67,120 - Ac3H11_178 0.73 -1.3 67,120 - Ac3H11_178 0.73 -3.6 67,120 - Ac3H11_178 0.73 -1.9 67,120 - Ac3H11_178 0.73 +0.3
Or see this region's nucleotide sequence