Experiment: Paraquat dichloride 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_99 and Ac3H11_100 are separated by 60 nucleotides Ac3H11_100 and Ac3H11_101 are separated by 68 nucleotides Ac3H11_101 and Ac3H11_102 are separated by 34 nucleotides Ac3H11_102 and Ac3H11_103 are separated by 1 nucleotides
Ac3H11_99 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 102,336 to 103,883
_99
Ac3H11_100 - Enoyl-CoA hydratase (EC 4.2.1.17), at 103,944 to 104,756
_100
Ac3H11_101 - Benzoate transport, extracellular ligand-binding receptor, at 104,825 to 105,994
_101
Ac3H11_102 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 106,029 to 106,910
_102
Ac3H11_103 - Benzoate transport, inner-membrane translocator precursor, at 106,912 to 107,961
_103
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 104.015 kb on - strand at 104.015 kb on - strand at 104.361 kb on + strand, within Ac3H11_100 at 104.362 kb on - strand, within Ac3H11_100 at 104.436 kb on - strand, within Ac3H11_100 at 104.506 kb on + strand, within Ac3H11_100 at 104.506 kb on + strand, within Ac3H11_100 at 104.597 kb on + strand, within Ac3H11_100 at 104.597 kb on + strand, within Ac3H11_100 at 104.670 kb on + strand, within Ac3H11_100 at 104.684 kb on + strand at 104.684 kb on + strand at 104.684 kb on + strand at 104.685 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.823 kb on - strand at 104.867 kb on - strand at 104.936 kb on + strand at 104.936 kb on + strand at 104.936 kb on + strand at 104.936 kb on + strand at 104.936 kb on + strand at 104.937 kb on - strand at 104.937 kb on - strand at 104.937 kb on - strand at 104.937 kb on - strand at 105.036 kb on - strand, within Ac3H11_101 at 105.266 kb on - strand, within Ac3H11_101 at 105.266 kb on - strand, within Ac3H11_101 at 105.266 kb on - strand, within Ac3H11_101 at 105.326 kb on + strand, within Ac3H11_101 at 105.327 kb on - strand, within Ac3H11_101 at 105.327 kb on - strand, within Ac3H11_101 at 105.443 kb on + strand, within Ac3H11_101 at 105.443 kb on + strand, within Ac3H11_101 at 105.443 kb on + strand, within Ac3H11_101 at 105.444 kb on - strand, within Ac3H11_101 at 105.491 kb on + strand, within Ac3H11_101 at 105.492 kb on - strand, within Ac3H11_101 at 105.492 kb on - strand, within Ac3H11_101 at 105.653 kb on + strand, within Ac3H11_101 at 105.749 kb on + strand, within Ac3H11_101 at 105.749 kb on + strand, within Ac3H11_101 at 105.750 kb on - strand, within Ac3H11_101 at 105.750 kb on - strand, within Ac3H11_101 at 105.767 kb on + strand, within Ac3H11_101 at 106.029 kb on - strand at 106.048 kb on + strand at 106.049 kb on - strand at 106.167 kb on + strand, within Ac3H11_102 at 106.167 kb on + strand, within Ac3H11_102 at 106.174 kb on + strand, within Ac3H11_102 at 106.182 kb on + strand, within Ac3H11_102 at 106.182 kb on + strand, within Ac3H11_102 at 106.182 kb on + strand, within Ac3H11_102 at 106.182 kb on + strand, within Ac3H11_102 at 106.183 kb on - strand, within Ac3H11_102 at 106.183 kb on - strand, within Ac3H11_102 at 106.458 kb on + strand, within Ac3H11_102 at 106.557 kb on + strand, within Ac3H11_102 at 106.557 kb on + strand, within Ac3H11_102 at 106.606 kb on - strand, within Ac3H11_102 at 106.671 kb on + strand, within Ac3H11_102 at 106.672 kb on - strand, within Ac3H11_102 at 106.809 kb on + strand, within Ac3H11_102 at 106.810 kb on - strand, within Ac3H11_102 at 106.810 kb on - strand, within Ac3H11_102
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.01 mg/ml remove 104,015 - +1.8 104,015 - -0.3 104,361 + Ac3H11_100 0.51 +0.2 104,362 - Ac3H11_100 0.51 +2.3 104,436 - Ac3H11_100 0.61 +0.8 104,506 + Ac3H11_100 0.69 +1.0 104,506 + Ac3H11_100 0.69 +0.8 104,597 + Ac3H11_100 0.80 +1.0 104,597 + Ac3H11_100 0.80 +0.4 104,670 + Ac3H11_100 0.89 +1.7 104,684 + +0.9 104,684 + +0.6 104,684 + -3.9 104,685 - +1.1 104,823 - +2.3 104,823 - +0.7 104,823 - +1.8 104,867 - -0.8 104,936 + +0.9 104,936 + -0.2 104,936 + +1.8 104,936 + +1.5 104,936 + +2.0 104,937 - -0.5 104,937 - +0.7 104,937 - +0.5 104,937 - +1.0 105,036 - Ac3H11_101 0.18 -1.2 105,266 - Ac3H11_101 0.38 -2.8 105,266 - Ac3H11_101 0.38 -0.7 105,266 - Ac3H11_101 0.38 +0.8 105,326 + Ac3H11_101 0.43 +0.7 105,327 - Ac3H11_101 0.43 -0.5 105,327 - Ac3H11_101 0.43 +0.7 105,443 + Ac3H11_101 0.53 -0.4 105,443 + Ac3H11_101 0.53 +0.6 105,443 + Ac3H11_101 0.53 -1.3 105,444 - Ac3H11_101 0.53 -2.1 105,491 + Ac3H11_101 0.57 +0.4 105,492 - Ac3H11_101 0.57 -2.1 105,492 - Ac3H11_101 0.57 -0.8 105,653 + Ac3H11_101 0.71 +0.2 105,749 + Ac3H11_101 0.79 -0.7 105,749 + Ac3H11_101 0.79 -1.1 105,750 - Ac3H11_101 0.79 +1.6 105,750 - Ac3H11_101 0.79 +0.1 105,767 + Ac3H11_101 0.81 -1.6 106,029 - -2.1 106,048 + -0.5 106,049 - -3.5 106,167 + Ac3H11_102 0.16 -3.4 106,167 + Ac3H11_102 0.16 -2.2 106,174 + Ac3H11_102 0.16 -3.3 106,182 + Ac3H11_102 0.17 +0.0 106,182 + Ac3H11_102 0.17 +0.5 106,182 + Ac3H11_102 0.17 +0.6 106,182 + Ac3H11_102 0.17 -0.5 106,183 - Ac3H11_102 0.17 +0.7 106,183 - Ac3H11_102 0.17 -0.8 106,458 + Ac3H11_102 0.49 -2.1 106,557 + Ac3H11_102 0.60 +0.3 106,557 + Ac3H11_102 0.60 +0.5 106,606 - Ac3H11_102 0.65 +0.8 106,671 + Ac3H11_102 0.73 -0.1 106,672 - Ac3H11_102 0.73 +1.1 106,809 + Ac3H11_102 0.88 -2.1 106,810 - Ac3H11_102 0.89 +3.1 106,810 - Ac3H11_102 0.89 +0.2
Or see this region's nucleotide sequence