Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panC and Shewana3_3410 are separated by 291 nucleotides Shewana3_3410 and Shewana3_3411 are separated by 61 nucleotides
Shewana3_3409: panC - pantoate--beta-alanine ligase (RefSeq), at 4,077,644 to 4,078,489
panC
Shewana3_3410: Shewana3_3410 - hypothetical protein (RefSeq), at 4,078,781 to 4,079,302
_3410
Shewana3_3411: Shewana3_3411 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 4,079,364 to 4,081,871
_3411
Position (kb)
4078
4079
4080 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.552 kb on - strand at 4078.665 kb on + strand at 4079.051 kb on - strand, within Shewana3_3410 at 4079.370 kb on - strand at 4079.370 kb on - strand at 4079.530 kb on + strand at 4079.656 kb on - strand, within Shewana3_3411 at 4079.682 kb on + strand, within Shewana3_3411 at 4079.894 kb on - strand, within Shewana3_3411 at 4080.022 kb on + strand, within Shewana3_3411 at 4080.057 kb on + strand, within Shewana3_3411 at 4080.065 kb on - strand, within Shewana3_3411 at 4080.065 kb on - strand, within Shewana3_3411 at 4080.171 kb on - strand, within Shewana3_3411 at 4080.253 kb on - strand, within Shewana3_3411
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 4,078,547 - -1.3 4,078,547 - -1.3 4,078,547 - +0.8 4,078,547 - -0.9 4,078,552 - -4.0 4,078,665 + +0.5 4,079,051 - Shewana3_3410 0.52 -2.2 4,079,370 - -0.4 4,079,370 - +0.8 4,079,530 + +1.5 4,079,656 - Shewana3_3411 0.12 +0.8 4,079,682 + Shewana3_3411 0.13 -1.6 4,079,894 - Shewana3_3411 0.21 -0.3 4,080,022 + Shewana3_3411 0.26 +0.2 4,080,057 + Shewana3_3411 0.28 +0.5 4,080,065 - Shewana3_3411 0.28 -0.6 4,080,065 - Shewana3_3411 0.28 +0.0 4,080,171 - Shewana3_3411 0.32 +0.5 4,080,253 - Shewana3_3411 0.35 +1.8
Or see this region's nucleotide sequence