Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 3 nucleotides hisG and Shewana3_1855 are separated by 659 nucleotides
Shewana3_1853: hisD - histidinol dehydrogenase (RefSeq), at 2,194,820 to 2,196,121
hisD
Shewana3_1854: hisG - ATP phosphoribosyltransferase (RefSeq), at 2,196,125 to 2,197,024
hisG
Shewana3_1855: Shewana3_1855 - hypothetical protein (RefSeq), at 2,197,684 to 2,198,511
_1855
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2195.272 kb on + strand, within hisD at 2195.369 kb on - strand, within hisD at 2195.825 kb on + strand, within hisD at 2195.825 kb on + strand, within hisD at 2195.924 kb on - strand, within hisD at 2196.027 kb on - strand at 2196.195 kb on - strand at 2196.195 kb on - strand at 2196.195 kb on - strand at 2196.380 kb on + strand, within hisG at 2196.380 kb on + strand, within hisG at 2196.398 kb on - strand, within hisG at 2196.444 kb on - strand, within hisG at 2196.446 kb on + strand, within hisG at 2196.475 kb on + strand, within hisG at 2196.966 kb on - strand at 2196.996 kb on + strand at 2197.026 kb on + strand at 2197.216 kb on - strand at 2197.237 kb on + strand at 2197.270 kb on - strand at 2197.326 kb on - strand at 2197.639 kb on + strand at 2197.666 kb on - strand at 2197.670 kb on - strand at 2197.741 kb on - strand at 2197.748 kb on + strand at 2197.800 kb on - strand, within Shewana3_1855 at 2197.810 kb on - strand, within Shewana3_1855 at 2197.836 kb on - strand, within Shewana3_1855 at 2197.859 kb on + strand, within Shewana3_1855 at 2197.859 kb on + strand, within Shewana3_1855 at 2197.867 kb on - strand, within Shewana3_1855 at 2197.877 kb on - strand, within Shewana3_1855 at 2197.896 kb on + strand, within Shewana3_1855 at 2197.901 kb on + strand, within Shewana3_1855 at 2197.909 kb on - strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.920 kb on + strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.928 kb on - strand, within Shewana3_1855 at 2197.935 kb on - strand, within Shewana3_1855 at 2197.937 kb on + strand, within Shewana3_1855 at 2197.945 kb on - strand, within Shewana3_1855 at 2197.968 kb on + strand, within Shewana3_1855 at 2198.002 kb on - strand, within Shewana3_1855
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,195,272 + hisD Shewana3_1853 0.35 -3.8 2,195,369 - hisD Shewana3_1853 0.42 -3.3 2,195,825 + hisD Shewana3_1853 0.77 -4.3 2,195,825 + hisD Shewana3_1853 0.77 -5.9 2,195,924 - hisD Shewana3_1853 0.85 -6.2 2,196,027 - -5.0 2,196,195 - -3.0 2,196,195 - -3.1 2,196,195 - -1.8 2,196,380 + hisG Shewana3_1854 0.28 -2.5 2,196,380 + hisG Shewana3_1854 0.28 -0.5 2,196,398 - hisG Shewana3_1854 0.30 -3.8 2,196,444 - hisG Shewana3_1854 0.35 -4.3 2,196,446 + hisG Shewana3_1854 0.36 -5.3 2,196,475 + hisG Shewana3_1854 0.39 -4.7 2,196,966 - -4.0 2,196,996 + -4.5 2,197,026 + -5.2 2,197,216 - -3.0 2,197,237 + -2.5 2,197,270 - -0.6 2,197,326 - -1.8 2,197,639 + +0.1 2,197,666 - -1.3 2,197,670 - -0.8 2,197,741 - +0.2 2,197,748 + -0.4 2,197,800 - Shewana3_1855 0.14 -0.1 2,197,810 - Shewana3_1855 0.15 -0.1 2,197,836 - Shewana3_1855 0.18 +1.0 2,197,859 + Shewana3_1855 0.21 +0.4 2,197,859 + Shewana3_1855 0.21 -2.3 2,197,867 - Shewana3_1855 0.22 -0.2 2,197,877 - Shewana3_1855 0.23 -2.6 2,197,896 + Shewana3_1855 0.26 +0.4 2,197,901 + Shewana3_1855 0.26 -2.3 2,197,909 - Shewana3_1855 0.27 -3.0 2,197,920 + Shewana3_1855 0.29 +1.4 2,197,920 + Shewana3_1855 0.29 +0.2 2,197,920 + Shewana3_1855 0.29 -0.8 2,197,920 + Shewana3_1855 0.29 -2.0 2,197,920 + Shewana3_1855 0.29 +0.1 2,197,920 + Shewana3_1855 0.29 +1.1 2,197,920 + Shewana3_1855 0.29 -0.6 2,197,920 + Shewana3_1855 0.29 +1.6 2,197,920 + Shewana3_1855 0.29 -0.2 2,197,928 - Shewana3_1855 0.29 -0.2 2,197,928 - Shewana3_1855 0.29 +0.2 2,197,928 - Shewana3_1855 0.29 +1.1 2,197,928 - Shewana3_1855 0.29 -0.2 2,197,928 - Shewana3_1855 0.29 +0.1 2,197,928 - Shewana3_1855 0.29 +0.0 2,197,928 - Shewana3_1855 0.29 +0.2 2,197,928 - Shewana3_1855 0.29 -2.7 2,197,928 - Shewana3_1855 0.29 +0.8 2,197,928 - Shewana3_1855 0.29 +2.3 2,197,928 - Shewana3_1855 0.29 -0.1 2,197,928 - Shewana3_1855 0.29 -1.1 2,197,928 - Shewana3_1855 0.29 -0.2 2,197,935 - Shewana3_1855 0.30 +0.6 2,197,937 + Shewana3_1855 0.31 +1.2 2,197,945 - Shewana3_1855 0.32 +0.5 2,197,968 + Shewana3_1855 0.34 +0.3 2,198,002 - Shewana3_1855 0.38 -0.9
Or see this region's nucleotide sequence