Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0980 and Shewana3_0981 are separated by 44 nucleotides Shewana3_0981 and Shewana3_R0030 are separated by 305 nucleotides Shewana3_R0030 and Shewana3_0982 are separated by 170 nucleotides Shewana3_0982 and Shewana3_0983 are separated by 60 nucleotides
Shewana3_0980: Shewana3_0980 - 3'-5' exonuclease (RefSeq), at 1,150,972 to 1,151,883
_0980
Shewana3_0981: Shewana3_0981 - hypothetical protein (RefSeq), at 1,151,928 to 1,152,896
_0981
Shewana3_R0030: Shewana3_R0030 - tRNA-Trp (RefSeq), at 1,153,202 to 1,153,278
_R0030
Shewana3_0982: Shewana3_0982 - hypothetical protein (RefSeq), at 1,153,449 to 1,153,727
_0982
Shewana3_0983: Shewana3_0983 - TonB-dependent siderophore receptor (RefSeq), at 1,153,788 to 1,155,959
_0983
Position (kb)
1151
1152
1153 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1151.215 kb on - strand, within Shewana3_0980 at 1151.366 kb on + strand, within Shewana3_0980 at 1151.374 kb on - strand, within Shewana3_0980 at 1151.374 kb on - strand, within Shewana3_0980 at 1151.477 kb on - strand, within Shewana3_0980 at 1151.539 kb on - strand, within Shewana3_0980 at 1151.841 kb on + strand at 1151.884 kb on + strand at 1152.081 kb on + strand, within Shewana3_0981 at 1152.264 kb on + strand, within Shewana3_0981 at 1152.344 kb on - strand, within Shewana3_0981 at 1152.407 kb on - strand, within Shewana3_0981 at 1152.638 kb on - strand, within Shewana3_0981 at 1152.762 kb on + strand, within Shewana3_0981 at 1153.079 kb on + strand at 1153.401 kb on - strand at 1153.437 kb on - strand at 1153.465 kb on + strand at 1153.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,151,215 - Shewana3_0980 0.27 +2.1 1,151,366 + Shewana3_0980 0.43 -3.0 1,151,374 - Shewana3_0980 0.44 -0.5 1,151,374 - Shewana3_0980 0.44 -0.8 1,151,477 - Shewana3_0980 0.55 +0.5 1,151,539 - Shewana3_0980 0.62 +0.1 1,151,841 + +0.0 1,151,884 + +0.3 1,152,081 + Shewana3_0981 0.16 -1.6 1,152,264 + Shewana3_0981 0.35 +0.2 1,152,344 - Shewana3_0981 0.43 -1.1 1,152,407 - Shewana3_0981 0.49 +2.9 1,152,638 - Shewana3_0981 0.73 +0.2 1,152,762 + Shewana3_0981 0.86 +2.6 1,153,079 + -0.1 1,153,401 - +0.7 1,153,437 - +0.7 1,153,465 + -0.6 1,153,835 - -0.2
Or see this region's nucleotide sequence