Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdhR and aceE are separated by 80 nucleotides aceE and Shewana3_0427 are separated by 12 nucleotides
Shewana3_0425: pdhR - transcriptional regulator PdhR (RefSeq), at 484,423 to 485,175
pdhR
Shewana3_0426: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 485,256 to 487,922
aceE
Shewana3_0427: Shewana3_0427 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (RefSeq), at 487,935 to 489,941
_0427
Position (kb) 485
486
487
488 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 484.513 kb on + strand, within pdhR at 484.918 kb on + strand, within pdhR at 485.241 kb on + strand at 485.271 kb on + strand at 485.278 kb on + strand at 485.314 kb on - strand at 485.325 kb on + strand at 485.408 kb on + strand at 485.416 kb on - strand at 485.800 kb on - strand, within aceE at 485.882 kb on + strand, within aceE at 485.989 kb on - strand, within aceE at 486.036 kb on - strand, within aceE at 486.068 kb on + strand, within aceE at 486.068 kb on + strand, within aceE at 486.305 kb on + strand, within aceE at 486.313 kb on - strand, within aceE at 486.369 kb on + strand, within aceE at 486.369 kb on + strand, within aceE at 486.369 kb on + strand, within aceE at 486.369 kb on + strand, within aceE at 486.369 kb on + strand, within aceE at 486.369 kb on + strand, within aceE at 486.369 kb on + strand, within aceE at 486.377 kb on - strand, within aceE at 486.459 kb on + strand, within aceE at 486.467 kb on - strand, within aceE at 486.627 kb on + strand, within aceE at 486.635 kb on - strand, within aceE at 486.713 kb on + strand, within aceE at 486.723 kb on - strand, within aceE at 487.017 kb on + strand, within aceE at 487.088 kb on + strand, within aceE at 487.096 kb on - strand, within aceE at 487.192 kb on - strand, within aceE at 487.210 kb on - strand, within aceE at 487.230 kb on - strand, within aceE at 487.493 kb on - strand, within aceE at 487.679 kb on + strand at 487.717 kb on + strand at 487.725 kb on + strand at 487.733 kb on - strand at 487.780 kb on + strand at 487.800 kb on - strand at 487.851 kb on + strand at 487.991 kb on - strand at 488.097 kb on + strand at 488.193 kb on + strand, within Shewana3_0427 at 488.340 kb on + strand, within Shewana3_0427 at 488.506 kb on + strand, within Shewana3_0427 at 488.514 kb on - strand, within Shewana3_0427 at 488.725 kb on + strand, within Shewana3_0427 at 488.733 kb on - strand, within Shewana3_0427 at 488.835 kb on + strand, within Shewana3_0427 at 488.914 kb on - strand, within Shewana3_0427
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 484,513 + pdhR Shewana3_0425 0.12 -1.7 484,918 + pdhR Shewana3_0425 0.66 +2.8 485,241 + -3.7 485,271 + -3.5 485,278 + -2.9 485,314 - -2.4 485,325 + -2.0 485,408 + -3.0 485,416 - -3.9 485,800 - aceE Shewana3_0426 0.20 -2.8 485,882 + aceE Shewana3_0426 0.23 -0.6 485,989 - aceE Shewana3_0426 0.27 -1.3 486,036 - aceE Shewana3_0426 0.29 -2.6 486,068 + aceE Shewana3_0426 0.30 -3.4 486,068 + aceE Shewana3_0426 0.30 -4.2 486,305 + aceE Shewana3_0426 0.39 -3.3 486,313 - aceE Shewana3_0426 0.40 -2.9 486,369 + aceE Shewana3_0426 0.42 -2.6 486,369 + aceE Shewana3_0426 0.42 -1.9 486,369 + aceE Shewana3_0426 0.42 -1.7 486,369 + aceE Shewana3_0426 0.42 -2.6 486,369 + aceE Shewana3_0426 0.42 -2.4 486,369 + aceE Shewana3_0426 0.42 -4.0 486,369 + aceE Shewana3_0426 0.42 -3.3 486,377 - aceE Shewana3_0426 0.42 -2.4 486,459 + aceE Shewana3_0426 0.45 -5.1 486,467 - aceE Shewana3_0426 0.45 -3.4 486,627 + aceE Shewana3_0426 0.51 -3.0 486,635 - aceE Shewana3_0426 0.52 -3.2 486,713 + aceE Shewana3_0426 0.55 -3.7 486,723 - aceE Shewana3_0426 0.55 -4.2 487,017 + aceE Shewana3_0426 0.66 -1.6 487,088 + aceE Shewana3_0426 0.69 -3.4 487,096 - aceE Shewana3_0426 0.69 -3.5 487,192 - aceE Shewana3_0426 0.73 -3.0 487,210 - aceE Shewana3_0426 0.73 -1.3 487,230 - aceE Shewana3_0426 0.74 -3.4 487,493 - aceE Shewana3_0426 0.84 -2.3 487,679 + -3.4 487,717 + -1.8 487,725 + -4.0 487,733 - -4.4 487,780 + -4.2 487,800 - -1.3 487,851 + -2.2 487,991 - +0.1 488,097 + -2.7 488,193 + Shewana3_0427 0.13 -3.3 488,340 + Shewana3_0427 0.20 -2.6 488,506 + Shewana3_0427 0.28 -1.3 488,514 - Shewana3_0427 0.29 -3.4 488,725 + Shewana3_0427 0.39 -4.4 488,733 - Shewana3_0427 0.40 -1.2 488,835 + Shewana3_0427 0.45 -2.9 488,914 - Shewana3_0427 0.49 -1.9
Or see this region's nucleotide sequence