Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0018 and Shewana3_R0019 are separated by 42 nucleotides Shewana3_R0019 and Shewana3_0239 are separated by 205 nucleotides Shewana3_0239 and Shewana3_0240 are separated by 38 nucleotides Shewana3_0240 and Shewana3_0241 are separated by 2 nucleotides Shewana3_0241 and Shewana3_0242 overlap by 1 nucleotides Shewana3_0242 and Shewana3_0243 overlap by 8 nucleotides
Shewana3_R0018: Shewana3_R0018 - 5S ribosomal RNA (RefSeq), at 272,229 to 272,348
_R0018
Shewana3_R0019: Shewana3_R0019 - tRNA-Asp (RefSeq), at 272,391 to 272,467
_R0019
Shewana3_0239: Shewana3_0239 - hypothetical protein (RefSeq), at 272,673 to 273,185
_0239
Shewana3_0240: Shewana3_0240 - ATPase domain-containing protein (RefSeq), at 273,224 to 274,540
_0240
Shewana3_0241: Shewana3_0241 - two component transcriptional regulator (RefSeq), at 274,543 to 275,205
_0241
Shewana3_0242: Shewana3_0242 - peptidase (RefSeq), at 275,205 to 275,471
_0242
Shewana3_0243: Shewana3_0243 - diheme cytochrome c (RefSeq), at 275,464 to 276,027
_0243
Position (kb) 273
274
275 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 273.051 kb on + strand, within Shewana3_0239 at 273.215 kb on + strand at 273.262 kb on - strand at 273.331 kb on + strand at 273.423 kb on + strand, within Shewana3_0240 at 273.431 kb on - strand, within Shewana3_0240 at 273.479 kb on - strand, within Shewana3_0240 at 273.611 kb on - strand, within Shewana3_0240 at 273.720 kb on - strand, within Shewana3_0240 at 273.825 kb on + strand, within Shewana3_0240 at 273.838 kb on - strand, within Shewana3_0240 at 273.991 kb on + strand, within Shewana3_0240 at 274.079 kb on - strand, within Shewana3_0240 at 274.126 kb on + strand, within Shewana3_0240 at 274.126 kb on + strand, within Shewana3_0240 at 274.493 kb on - strand at 274.783 kb on - strand, within Shewana3_0241 at 274.804 kb on + strand, within Shewana3_0241 at 274.843 kb on + strand, within Shewana3_0241 at 275.022 kb on - strand, within Shewana3_0241 at 275.161 kb on - strand at 275.377 kb on - strand, within Shewana3_0242 at 275.377 kb on - strand, within Shewana3_0242 at 275.377 kb on - strand, within Shewana3_0242 at 275.392 kb on + strand, within Shewana3_0242 at 275.454 kb on + strand at 275.540 kb on - strand, within Shewana3_0243
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 273,051 + Shewana3_0239 0.74 -0.3 273,215 + +0.5 273,262 - +0.3 273,331 + +0.2 273,423 + Shewana3_0240 0.15 +1.0 273,431 - Shewana3_0240 0.16 +0.8 273,479 - Shewana3_0240 0.19 +0.1 273,611 - Shewana3_0240 0.29 +0.3 273,720 - Shewana3_0240 0.38 -0.5 273,825 + Shewana3_0240 0.46 -1.1 273,838 - Shewana3_0240 0.47 -0.9 273,991 + Shewana3_0240 0.58 -4.4 274,079 - Shewana3_0240 0.65 -1.0 274,126 + Shewana3_0240 0.68 +0.6 274,126 + Shewana3_0240 0.68 -1.5 274,493 - +1.0 274,783 - Shewana3_0241 0.36 -1.0 274,804 + Shewana3_0241 0.39 +1.4 274,843 + Shewana3_0241 0.45 +1.1 275,022 - Shewana3_0241 0.72 +0.4 275,161 - -1.2 275,377 - Shewana3_0242 0.64 -1.7 275,377 - Shewana3_0242 0.64 -5.7 275,377 - Shewana3_0242 0.64 -3.0 275,392 + Shewana3_0242 0.70 +0.0 275,454 + -3.2 275,540 - Shewana3_0243 0.13 +0.7
Or see this region's nucleotide sequence