Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2119 and Synpcc7942_2120 are separated by 41 nucleotides Synpcc7942_2120 and spoIIA are separated by 2 nucleotides spoIIA and carA are separated by 50 nucleotides
Synpcc7942_2119: Synpcc7942_2119 - RNA methyltransferase TrmH, group 3, at 2,200,097 to 2,201,656
_2119
Synpcc7942_2120: Synpcc7942_2120 - Ribonuclease III, at 2,201,698 to 2,202,114
_2120
Synpcc7942_2121: spoIIA - anti-anti-sigma regulatory factor, SpoIIAA, at 2,202,117 to 2,202,509
spoIIA
Synpcc7942_2122: carA - carbamoyl phosphate synthase small subunit, at 2,202,560 to 2,203,735
carA
Position (kb)
2201
2202
2203 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2201.339 kb on + strand, within Synpcc7942_2119 at 2201.697 kb on - strand at 2201.757 kb on + strand, within Synpcc7942_2120 at 2201.757 kb on + strand, within Synpcc7942_2120 at 2201.765 kb on - strand, within Synpcc7942_2120 at 2201.765 kb on - strand, within Synpcc7942_2120 at 2201.775 kb on - strand, within Synpcc7942_2120 at 2201.778 kb on + strand, within Synpcc7942_2120 at 2201.779 kb on + strand, within Synpcc7942_2120 at 2201.779 kb on + strand, within Synpcc7942_2120 at 2201.787 kb on - strand, within Synpcc7942_2120 at 2201.787 kb on - strand, within Synpcc7942_2120 at 2201.879 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.897 kb on - strand, within Synpcc7942_2120 at 2202.018 kb on + strand, within Synpcc7942_2120 at 2202.026 kb on - strand, within Synpcc7942_2120 at 2202.028 kb on + strand, within Synpcc7942_2120 at 2202.029 kb on + strand, within Synpcc7942_2120 at 2202.060 kb on + strand, within Synpcc7942_2120 at 2202.093 kb on + strand at 2202.093 kb on + strand at 2202.100 kb on - strand at 2202.101 kb on - strand at 2202.546 kb on + strand at 2202.557 kb on + strand at 2202.566 kb on - strand at 2202.566 kb on - strand at 2202.566 kb on - strand at 2202.575 kb on + strand at 2202.590 kb on + strand at 2202.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 2,201,339 + Synpcc7942_2119 0.80 +1.4 2,201,697 - -0.3 2,201,757 + Synpcc7942_2120 0.14 -3.9 2,201,757 + Synpcc7942_2120 0.14 -0.6 2,201,765 - Synpcc7942_2120 0.16 -2.8 2,201,765 - Synpcc7942_2120 0.16 -2.5 2,201,775 - Synpcc7942_2120 0.18 +0.1 2,201,778 + Synpcc7942_2120 0.19 -1.5 2,201,779 + Synpcc7942_2120 0.19 -0.1 2,201,779 + Synpcc7942_2120 0.19 +0.2 2,201,787 - Synpcc7942_2120 0.21 -1.1 2,201,787 - Synpcc7942_2120 0.21 -0.9 2,201,879 + Synpcc7942_2120 0.43 +0.1 2,201,889 + Synpcc7942_2120 0.46 -2.3 2,201,889 + Synpcc7942_2120 0.46 +0.2 2,201,889 + Synpcc7942_2120 0.46 -1.0 2,201,889 + Synpcc7942_2120 0.46 +0.3 2,201,897 - Synpcc7942_2120 0.48 -1.8 2,202,018 + Synpcc7942_2120 0.77 -2.3 2,202,026 - Synpcc7942_2120 0.79 -0.5 2,202,028 + Synpcc7942_2120 0.79 -1.3 2,202,029 + Synpcc7942_2120 0.79 -0.6 2,202,060 + Synpcc7942_2120 0.87 -1.2 2,202,093 + -0.9 2,202,093 + -1.0 2,202,100 - -0.1 2,202,101 - -1.9 2,202,546 + +0.5 2,202,557 + +0.4 2,202,566 - -3.6 2,202,566 - -0.4 2,202,566 - -1.9 2,202,575 + -0.9 2,202,590 + -1.5 2,202,605 - +0.3
Or see this region's nucleotide sequence