Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisIE and Synpcc7942_1996 are separated by 55 nucleotides Synpcc7942_1996 and hliA are separated by 184 nucleotides hliA and Synpcc7942_1998 are separated by 185 nucleotides
Synpcc7942_1995: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein, at 2,064,926 to 2,065,561
hisIE
Synpcc7942_1996: Synpcc7942_1996 - hypothetical protein, at 2,065,617 to 2,065,967
_1996
Synpcc7942_1997: hliA - high light-inducible protein, at 2,066,152 to 2,066,370
hliA
Synpcc7942_1998: Synpcc7942_1998 - GAF, at 2,066,556 to 2,067,143
_1998
Position (kb)
2066
2067 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2065.596 kb on - strand at 2065.597 kb on - strand at 2065.625 kb on + strand at 2065.625 kb on + strand at 2065.635 kb on - strand at 2065.679 kb on - strand, within Synpcc7942_1996 at 2065.686 kb on - strand, within Synpcc7942_1996 at 2065.704 kb on - strand, within Synpcc7942_1996 at 2065.717 kb on + strand, within Synpcc7942_1996 at 2065.754 kb on + strand, within Synpcc7942_1996 at 2065.756 kb on + strand, within Synpcc7942_1996 at 2065.763 kb on - strand, within Synpcc7942_1996 at 2065.789 kb on + strand, within Synpcc7942_1996 at 2065.831 kb on - strand, within Synpcc7942_1996 at 2065.831 kb on - strand, within Synpcc7942_1996 at 2065.844 kb on - strand, within Synpcc7942_1996 at 2065.893 kb on + strand, within Synpcc7942_1996 at 2065.902 kb on - strand, within Synpcc7942_1996 at 2065.941 kb on - strand at 2065.971 kb on + strand at 2065.981 kb on + strand at 2066.096 kb on + strand at 2066.113 kb on + strand at 2066.133 kb on + strand at 2066.133 kb on + strand at 2066.141 kb on - strand at 2066.149 kb on + strand at 2066.212 kb on + strand, within hliA at 2066.245 kb on + strand, within hliA at 2066.463 kb on + strand at 2066.532 kb on - strand at 2066.539 kb on - strand at 2066.539 kb on - strand at 2066.552 kb on + strand at 2066.552 kb on + strand at 2066.594 kb on + strand at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.630 kb on + strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.635 kb on + strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.643 kb on - strand, within Synpcc7942_1998 at 2066.657 kb on + strand, within Synpcc7942_1998 at 2066.681 kb on + strand, within Synpcc7942_1998 at 2066.689 kb on + strand at 2066.712 kb on - strand, within Synpcc7942_1998 at 2066.723 kb on - strand, within Synpcc7942_1998 at 2066.739 kb on - strand, within Synpcc7942_1998 at 2066.744 kb on - strand, within Synpcc7942_1998 at 2066.749 kb on - strand, within Synpcc7942_1998 at 2066.762 kb on - strand, within Synpcc7942_1998 at 2066.789 kb on + strand, within Synpcc7942_1998 at 2066.797 kb on - strand, within Synpcc7942_1998 at 2066.810 kb on + strand, within Synpcc7942_1998 at 2066.813 kb on - strand, within Synpcc7942_1998 at 2066.830 kb on + strand, within Synpcc7942_1998 at 2066.836 kb on - strand, within Synpcc7942_1998 at 2066.850 kb on + strand, within Synpcc7942_1998 at 2066.875 kb on + strand, within Synpcc7942_1998 at 2066.876 kb on + strand, within Synpcc7942_1998 at 2066.881 kb on + strand, within Synpcc7942_1998 at 2066.889 kb on - strand, within Synpcc7942_1998 at 2066.892 kb on + strand, within Synpcc7942_1998 at 2066.901 kb on + strand, within Synpcc7942_1998 at 2066.929 kb on - strand, within Synpcc7942_1998 at 2066.968 kb on - strand at 2066.968 kb on - strand, within Synpcc7942_1998 at 2066.968 kb on - strand, within Synpcc7942_1998 at 2066.968 kb on - strand, within Synpcc7942_1998 at 2067.002 kb on - strand, within Synpcc7942_1998 at 2067.008 kb on + strand, within Synpcc7942_1998 at 2067.025 kb on + strand, within Synpcc7942_1998 at 2067.039 kb on + strand, within Synpcc7942_1998 at 2067.044 kb on + strand, within Synpcc7942_1998 at 2067.052 kb on - strand, within Synpcc7942_1998 at 2067.052 kb on - strand, within Synpcc7942_1998 at 2067.076 kb on - strand, within Synpcc7942_1998 at 2067.107 kb on - strand at 2067.112 kb on + strand at 2067.178 kb on + strand at 2067.210 kb on + strand at 2067.216 kb on + strand at 2067.223 kb on - strand at 2067.223 kb on - strand at 2067.225 kb on - strand at 2067.236 kb on + strand at 2067.244 kb on - strand at 2067.245 kb on - strand at 2067.246 kb on + strand at 2067.246 kb on + strand at 2067.251 kb on + strand at 2067.251 kb on + strand at 2067.251 kb on + strand at 2067.254 kb on - strand at 2067.259 kb on - strand at 2067.259 kb on - strand at 2067.284 kb on - strand at 2067.293 kb on + strand at 2067.330 kb on - strand at 2067.345 kb on + strand at 2067.345 kb on + strand at 2067.346 kb on - strand at 2067.348 kb on - strand at 2067.348 kb on - strand at 2067.350 kb on + strand at 2067.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 2,065,596 - +0.3 2,065,597 - +0.8 2,065,625 + +0.5 2,065,625 + -2.5 2,065,635 - +0.5 2,065,679 - Synpcc7942_1996 0.18 -0.0 2,065,686 - Synpcc7942_1996 0.20 +0.3 2,065,704 - Synpcc7942_1996 0.25 -0.1 2,065,717 + Synpcc7942_1996 0.28 +0.7 2,065,754 + Synpcc7942_1996 0.39 -1.6 2,065,756 + Synpcc7942_1996 0.40 -2.8 2,065,763 - Synpcc7942_1996 0.42 -0.4 2,065,789 + Synpcc7942_1996 0.49 -2.0 2,065,831 - Synpcc7942_1996 0.61 +0.8 2,065,831 - Synpcc7942_1996 0.61 -1.4 2,065,844 - Synpcc7942_1996 0.65 -1.1 2,065,893 + Synpcc7942_1996 0.79 -3.9 2,065,902 - Synpcc7942_1996 0.81 +1.9 2,065,941 - +0.6 2,065,971 + -0.1 2,065,981 + -3.1 2,066,096 + -0.2 2,066,113 + -0.3 2,066,133 + -1.4 2,066,133 + +1.7 2,066,141 - +0.1 2,066,149 + +2.1 2,066,212 + hliA Synpcc7942_1997 0.27 -0.5 2,066,245 + hliA Synpcc7942_1997 0.42 -2.9 2,066,463 + +0.4 2,066,532 - +0.5 2,066,539 - +0.4 2,066,539 - +0.4 2,066,552 + +0.8 2,066,552 + +0.5 2,066,594 + +0.7 2,066,625 + Synpcc7942_1998 0.12 -0.2 2,066,625 + Synpcc7942_1998 0.12 +0.9 2,066,625 + Synpcc7942_1998 0.12 +1.6 2,066,630 + Synpcc7942_1998 0.13 -2.7 2,066,633 - Synpcc7942_1998 0.13 +2.8 2,066,633 - Synpcc7942_1998 0.13 -3.0 2,066,635 + Synpcc7942_1998 0.13 +0.5 2,066,641 - Synpcc7942_1998 0.14 -3.8 2,066,641 - Synpcc7942_1998 0.14 -3.7 2,066,643 - Synpcc7942_1998 0.15 -0.2 2,066,657 + Synpcc7942_1998 0.17 -2.4 2,066,681 + Synpcc7942_1998 0.21 +0.6 2,066,689 + -2.1 2,066,712 - Synpcc7942_1998 0.27 -2.5 2,066,723 - Synpcc7942_1998 0.28 +0.5 2,066,739 - Synpcc7942_1998 0.31 +0.2 2,066,744 - Synpcc7942_1998 0.32 -0.6 2,066,749 - Synpcc7942_1998 0.33 -1.1 2,066,762 - Synpcc7942_1998 0.35 +1.3 2,066,789 + Synpcc7942_1998 0.40 -2.2 2,066,797 - Synpcc7942_1998 0.41 +1.1 2,066,810 + Synpcc7942_1998 0.43 -1.7 2,066,813 - Synpcc7942_1998 0.44 +0.7 2,066,830 + Synpcc7942_1998 0.47 +1.8 2,066,836 - Synpcc7942_1998 0.48 +0.6 2,066,850 + Synpcc7942_1998 0.50 -1.2 2,066,875 + Synpcc7942_1998 0.54 -2.1 2,066,876 + Synpcc7942_1998 0.54 +0.3 2,066,881 + Synpcc7942_1998 0.55 -0.1 2,066,889 - Synpcc7942_1998 0.57 -2.2 2,066,892 + Synpcc7942_1998 0.57 -3.2 2,066,901 + Synpcc7942_1998 0.59 +0.5 2,066,929 - Synpcc7942_1998 0.63 +0.3 2,066,968 - -0.7 2,066,968 - Synpcc7942_1998 0.70 +0.9 2,066,968 - Synpcc7942_1998 0.70 +0.1 2,066,968 - Synpcc7942_1998 0.70 -0.3 2,067,002 - Synpcc7942_1998 0.76 +0.0 2,067,008 + Synpcc7942_1998 0.77 +0.6 2,067,025 + Synpcc7942_1998 0.80 -2.5 2,067,039 + Synpcc7942_1998 0.82 +1.1 2,067,044 + Synpcc7942_1998 0.83 +0.7 2,067,052 - Synpcc7942_1998 0.84 -3.2 2,067,052 - Synpcc7942_1998 0.84 +0.7 2,067,076 - Synpcc7942_1998 0.88 +1.1 2,067,107 - -1.5 2,067,112 + +0.8 2,067,178 + -0.9 2,067,210 + +0.9 2,067,216 + -3.2 2,067,223 - -2.4 2,067,223 - +1.1 2,067,225 - +0.2 2,067,236 + +0.2 2,067,244 - -0.5 2,067,245 - +0.9 2,067,246 + -1.1 2,067,246 + +0.3 2,067,251 + -3.1 2,067,251 + +0.1 2,067,251 + +0.3 2,067,254 - +1.2 2,067,259 - +0.3 2,067,259 - -0.8 2,067,284 - +0.4 2,067,293 + +0.5 2,067,330 - -0.2 2,067,345 + -0.3 2,067,345 + +0.4 2,067,346 - +0.1 2,067,348 - +1.6 2,067,348 - -0.8 2,067,350 + +0.3 2,067,353 - +1.1
Or see this region's nucleotide sequence