Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1859 and ycf29 overlap by 12 nucleotides ycf29 and natB are separated by 157 nucleotides
Synpcc7942_1859: Synpcc7942_1859 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s), at 1,927,733 to 1,930,486
_1859
Synpcc7942_1860: ycf29 - two component transcriptional regulator, LuxR family, at 1,930,475 to 1,931,182
ycf29
Synpcc7942_1861: natB - periplasmic binding protein of ABC transporter for natural amino acids, at 1,931,340 to 1,932,605
natB
Position (kb)
1930
1931
1932 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1929.555 kb on + strand, within Synpcc7942_1859 at 1929.563 kb on - strand, within Synpcc7942_1859 at 1929.599 kb on - strand, within Synpcc7942_1859 at 1929.636 kb on - strand, within Synpcc7942_1859 at 1929.636 kb on - strand, within Synpcc7942_1859 at 1929.700 kb on - strand, within Synpcc7942_1859 at 1929.732 kb on + strand, within Synpcc7942_1859 at 1929.740 kb on - strand, within Synpcc7942_1859 at 1929.740 kb on - strand, within Synpcc7942_1859 at 1929.745 kb on - strand, within Synpcc7942_1859 at 1929.802 kb on + strand, within Synpcc7942_1859 at 1929.812 kb on + strand, within Synpcc7942_1859 at 1929.823 kb on + strand, within Synpcc7942_1859 at 1929.831 kb on - strand, within Synpcc7942_1859 at 1929.859 kb on + strand, within Synpcc7942_1859 at 1929.927 kb on + strand, within Synpcc7942_1859 at 1929.993 kb on - strand, within Synpcc7942_1859 at 1930.016 kb on - strand, within Synpcc7942_1859 at 1930.068 kb on + strand, within Synpcc7942_1859 at 1930.097 kb on - strand, within Synpcc7942_1859 at 1930.166 kb on + strand, within Synpcc7942_1859 at 1930.193 kb on + strand, within Synpcc7942_1859 at 1930.201 kb on - strand, within Synpcc7942_1859 at 1930.257 kb on - strand at 1930.315 kb on + strand at 1930.318 kb on - strand at 1930.353 kb on + strand at 1930.418 kb on - strand at 1930.478 kb on + strand at 1931.194 kb on + strand at 1931.194 kb on + strand at 1931.196 kb on + strand at 1931.200 kb on - strand at 1931.202 kb on - strand at 1931.233 kb on - strand at 1931.241 kb on + strand at 1931.248 kb on - strand at 1931.248 kb on - strand at 1931.248 kb on - strand at 1931.248 kb on - strand at 1931.260 kb on - strand at 1931.279 kb on + strand at 1931.281 kb on - strand at 1931.287 kb on - strand at 1931.293 kb on - strand at 1931.298 kb on + strand at 1931.306 kb on - strand at 1931.320 kb on + strand at 1931.350 kb on - strand at 1931.350 kb on - strand at 1931.364 kb on - strand at 1931.364 kb on - strand at 1931.398 kb on + strand at 1931.407 kb on - strand at 1931.417 kb on - strand at 1931.464 kb on - strand at 1931.464 kb on - strand at 1931.484 kb on + strand, within natB at 1931.490 kb on + strand, within natB at 1931.491 kb on + strand, within natB at 1931.492 kb on - strand, within natB at 1931.492 kb on - strand, within natB at 1931.496 kb on + strand, within natB at 1931.497 kb on - strand, within natB at 1931.505 kb on - strand, within natB at 1931.536 kb on - strand, within natB at 1931.553 kb on + strand, within natB at 1931.553 kb on + strand, within natB at 1931.559 kb on - strand, within natB at 1931.570 kb on - strand, within natB at 1931.578 kb on + strand, within natB at 1931.591 kb on - strand at 1931.595 kb on + strand, within natB at 1931.602 kb on + strand, within natB at 1931.682 kb on - strand, within natB at 1931.717 kb on - strand, within natB at 1931.746 kb on - strand, within natB at 1931.748 kb on + strand, within natB at 1931.755 kb on + strand, within natB at 1931.756 kb on - strand, within natB at 1931.756 kb on - strand, within natB at 1931.761 kb on + strand, within natB at 1931.778 kb on - strand, within natB at 1931.788 kb on + strand, within natB at 1931.790 kb on + strand, within natB at 1931.790 kb on + strand, within natB at 1931.796 kb on - strand, within natB at 1931.798 kb on - strand, within natB at 1931.811 kb on + strand, within natB at 1931.825 kb on + strand, within natB at 1931.834 kb on - strand, within natB at 1931.840 kb on - strand, within natB at 1931.887 kb on + strand, within natB at 1931.899 kb on + strand, within natB at 1931.899 kb on + strand, within natB at 1931.932 kb on - strand, within natB at 1931.937 kb on + strand, within natB at 1931.945 kb on - strand, within natB at 1931.949 kb on + strand, within natB at 1931.951 kb on + strand, within natB at 1931.956 kb on + strand, within natB at 1931.959 kb on + strand, within natB at 1931.959 kb on - strand, within natB at 1931.961 kb on + strand, within natB at 1931.964 kb on - strand, within natB at 1931.967 kb on - strand, within natB at 1931.982 kb on + strand, within natB at 1931.990 kb on - strand, within natB at 1932.003 kb on - strand at 1932.005 kb on + strand at 1932.013 kb on - strand, within natB at 1932.035 kb on + strand, within natB at 1932.038 kb on - strand, within natB at 1932.066 kb on + strand, within natB at 1932.074 kb on - strand, within natB at 1932.085 kb on + strand, within natB at 1932.118 kb on - strand, within natB at 1932.118 kb on - strand, within natB at 1932.118 kb on - strand, within natB at 1932.123 kb on - strand, within natB at 1932.128 kb on + strand, within natB at 1932.132 kb on + strand, within natB at 1932.132 kb on + strand, within natB
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 1,929,555 + Synpcc7942_1859 0.66 +1.0 1,929,563 - Synpcc7942_1859 0.66 -2.2 1,929,599 - Synpcc7942_1859 0.68 +1.0 1,929,636 - Synpcc7942_1859 0.69 -1.9 1,929,636 - Synpcc7942_1859 0.69 -0.2 1,929,700 - Synpcc7942_1859 0.71 -0.2 1,929,732 + Synpcc7942_1859 0.73 +1.0 1,929,740 - Synpcc7942_1859 0.73 -0.8 1,929,740 - Synpcc7942_1859 0.73 -2.2 1,929,745 - Synpcc7942_1859 0.73 +1.4 1,929,802 + Synpcc7942_1859 0.75 +0.2 1,929,812 + Synpcc7942_1859 0.75 -0.5 1,929,823 + Synpcc7942_1859 0.76 -0.9 1,929,831 - Synpcc7942_1859 0.76 +1.3 1,929,859 + Synpcc7942_1859 0.77 -1.7 1,929,927 + Synpcc7942_1859 0.80 +0.9 1,929,993 - Synpcc7942_1859 0.82 +1.1 1,930,016 - Synpcc7942_1859 0.83 +0.5 1,930,068 + Synpcc7942_1859 0.85 +0.5 1,930,097 - Synpcc7942_1859 0.86 -0.2 1,930,166 + Synpcc7942_1859 0.88 +0.1 1,930,193 + Synpcc7942_1859 0.89 +0.4 1,930,201 - Synpcc7942_1859 0.90 +1.5 1,930,257 - -0.4 1,930,315 + +0.2 1,930,318 - +1.7 1,930,353 + +0.4 1,930,418 - +0.6 1,930,478 + -0.0 1,931,194 + -2.4 1,931,194 + -2.3 1,931,196 + +0.8 1,931,200 - +0.5 1,931,202 - +0.0 1,931,233 - +1.5 1,931,241 + -2.1 1,931,248 - +1.3 1,931,248 - -2.5 1,931,248 - -0.1 1,931,248 - +0.0 1,931,260 - +0.5 1,931,279 + -1.4 1,931,281 - +2.0 1,931,287 - +0.8 1,931,293 - -2.5 1,931,298 + -0.4 1,931,306 - +0.5 1,931,320 + -1.5 1,931,350 - +0.3 1,931,350 - +1.4 1,931,364 - +0.4 1,931,364 - +1.1 1,931,398 + +0.5 1,931,407 - +2.0 1,931,417 - +1.8 1,931,464 - -3.1 1,931,464 - -0.3 1,931,484 + natB Synpcc7942_1861 0.11 -1.9 1,931,490 + natB Synpcc7942_1861 0.12 +0.2 1,931,491 + natB Synpcc7942_1861 0.12 +0.8 1,931,492 - natB Synpcc7942_1861 0.12 -0.9 1,931,492 - natB Synpcc7942_1861 0.12 +0.3 1,931,496 + natB Synpcc7942_1861 0.12 -3.1 1,931,497 - natB Synpcc7942_1861 0.12 -1.2 1,931,505 - natB Synpcc7942_1861 0.13 -0.2 1,931,536 - natB Synpcc7942_1861 0.15 -0.4 1,931,553 + natB Synpcc7942_1861 0.17 +0.2 1,931,553 + natB Synpcc7942_1861 0.17 +0.5 1,931,559 - natB Synpcc7942_1861 0.17 -0.3 1,931,570 - natB Synpcc7942_1861 0.18 +0.3 1,931,578 + natB Synpcc7942_1861 0.19 -1.9 1,931,591 - -1.4 1,931,595 + natB Synpcc7942_1861 0.20 -0.5 1,931,602 + natB Synpcc7942_1861 0.21 +0.3 1,931,682 - natB Synpcc7942_1861 0.27 -3.2 1,931,717 - natB Synpcc7942_1861 0.30 -2.1 1,931,746 - natB Synpcc7942_1861 0.32 +0.1 1,931,748 + natB Synpcc7942_1861 0.32 +1.6 1,931,755 + natB Synpcc7942_1861 0.33 -1.3 1,931,756 - natB Synpcc7942_1861 0.33 -1.3 1,931,756 - natB Synpcc7942_1861 0.33 -1.3 1,931,761 + natB Synpcc7942_1861 0.33 +0.4 1,931,778 - natB Synpcc7942_1861 0.35 -1.5 1,931,788 + natB Synpcc7942_1861 0.35 -0.4 1,931,790 + natB Synpcc7942_1861 0.36 +0.1 1,931,790 + natB Synpcc7942_1861 0.36 +0.5 1,931,796 - natB Synpcc7942_1861 0.36 -0.2 1,931,798 - natB Synpcc7942_1861 0.36 +0.3 1,931,811 + natB Synpcc7942_1861 0.37 +0.2 1,931,825 + natB Synpcc7942_1861 0.38 -0.2 1,931,834 - natB Synpcc7942_1861 0.39 +0.6 1,931,840 - natB Synpcc7942_1861 0.39 -0.5 1,931,887 + natB Synpcc7942_1861 0.43 -3.2 1,931,899 + natB Synpcc7942_1861 0.44 +1.4 1,931,899 + natB Synpcc7942_1861 0.44 +0.6 1,931,932 - natB Synpcc7942_1861 0.47 -0.0 1,931,937 + natB Synpcc7942_1861 0.47 +0.6 1,931,945 - natB Synpcc7942_1861 0.48 -2.1 1,931,949 + natB Synpcc7942_1861 0.48 +0.2 1,931,951 + natB Synpcc7942_1861 0.48 +0.3 1,931,956 + natB Synpcc7942_1861 0.49 +0.9 1,931,959 + natB Synpcc7942_1861 0.49 +0.2 1,931,959 - natB Synpcc7942_1861 0.49 +0.1 1,931,961 + natB Synpcc7942_1861 0.49 +0.5 1,931,964 - natB Synpcc7942_1861 0.49 +2.1 1,931,967 - natB Synpcc7942_1861 0.50 +0.6 1,931,982 + natB Synpcc7942_1861 0.51 +0.5 1,931,990 - natB Synpcc7942_1861 0.51 +1.7 1,932,003 - -2.2 1,932,005 + +2.6 1,932,013 - natB Synpcc7942_1861 0.53 +0.2 1,932,035 + natB Synpcc7942_1861 0.55 +2.2 1,932,038 - natB Synpcc7942_1861 0.55 -3.2 1,932,066 + natB Synpcc7942_1861 0.57 -2.1 1,932,074 - natB Synpcc7942_1861 0.58 -0.2 1,932,085 + natB Synpcc7942_1861 0.59 -0.0 1,932,118 - natB Synpcc7942_1861 0.61 -0.8 1,932,118 - natB Synpcc7942_1861 0.61 +0.1 1,932,118 - natB Synpcc7942_1861 0.61 +1.4 1,932,123 - natB Synpcc7942_1861 0.62 +0.7 1,932,128 + natB Synpcc7942_1861 0.62 -0.0 1,932,132 + natB Synpcc7942_1861 0.63 -3.9 1,932,132 + natB Synpcc7942_1861 0.63 +0.9
Or see this region's nucleotide sequence