Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1006 and fumC are separated by 780 nucleotides fumC and purU are separated by 36 nucleotides
Synpcc7942_1006: Synpcc7942_1006 - TPR repeat, at 1,017,044 to 1,018,123
_1006
Synpcc7942_1007: fumC - fumarate hydratase, at 1,018,904 to 1,020,295
fumC
Synpcc7942_1008: purU - formyltetrahydrofolate deformylase, at 1,020,332 to 1,021,186
purU
Position (kb)
1018
1019
1020
1021 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1018.052 kb on + strand at 1018.145 kb on + strand at 1018.325 kb on + strand at 1018.510 kb on + strand at 1018.510 kb on + strand at 1018.518 kb on - strand at 1018.518 kb on - strand at 1018.608 kb on - strand at 1019.191 kb on - strand, within fumC at 1019.262 kb on + strand, within fumC at 1019.270 kb on - strand, within fumC at 1019.270 kb on - strand, within fumC at 1019.270 kb on - strand, within fumC at 1019.423 kb on + strand, within fumC at 1019.493 kb on + strand, within fumC at 1019.606 kb on - strand, within fumC at 1019.634 kb on + strand, within fumC at 1019.706 kb on - strand, within fumC at 1019.706 kb on - strand, within fumC at 1019.707 kb on - strand, within fumC at 1019.708 kb on - strand, within fumC at 1019.780 kb on + strand, within fumC at 1019.782 kb on + strand, within fumC at 1019.788 kb on - strand, within fumC at 1019.796 kb on + strand, within fumC at 1019.799 kb on - strand, within fumC at 1019.926 kb on - strand, within fumC at 1019.936 kb on - strand, within fumC at 1020.150 kb on - strand, within fumC at 1020.203 kb on + strand at 1020.219 kb on + strand at 1020.219 kb on + strand at 1020.252 kb on - strand at 1020.280 kb on - strand at 1020.338 kb on - strand at 1020.348 kb on + strand at 1020.365 kb on - strand at 1020.422 kb on + strand, within purU at 1020.472 kb on + strand, within purU at 1020.521 kb on + strand, within purU at 1020.528 kb on + strand, within purU at 1020.571 kb on - strand, within purU at 1020.917 kb on - strand, within purU at 1020.955 kb on + strand, within purU at 1020.957 kb on + strand, within purU at 1021.033 kb on - strand, within purU at 1021.033 kb on - strand, within purU at 1021.054 kb on - strand, within purU at 1021.083 kb on + strand, within purU at 1021.152 kb on - strand at 1021.157 kb on + strand at 1021.227 kb on + strand at 1021.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 1,018,052 + +0.2 1,018,145 + +0.9 1,018,325 + +0.6 1,018,510 + -0.2 1,018,510 + +1.0 1,018,518 - +0.2 1,018,518 - -0.2 1,018,608 - +0.5 1,019,191 - fumC Synpcc7942_1007 0.21 -3.7 1,019,262 + fumC Synpcc7942_1007 0.26 -2.1 1,019,270 - fumC Synpcc7942_1007 0.26 -0.5 1,019,270 - fumC Synpcc7942_1007 0.26 -3.0 1,019,270 - fumC Synpcc7942_1007 0.26 -2.1 1,019,423 + fumC Synpcc7942_1007 0.37 -0.5 1,019,493 + fumC Synpcc7942_1007 0.42 -0.2 1,019,606 - fumC Synpcc7942_1007 0.50 -0.3 1,019,634 + fumC Synpcc7942_1007 0.52 -2.0 1,019,706 - fumC Synpcc7942_1007 0.58 -1.5 1,019,706 - fumC Synpcc7942_1007 0.58 -0.7 1,019,707 - fumC Synpcc7942_1007 0.58 -0.4 1,019,708 - fumC Synpcc7942_1007 0.58 -2.9 1,019,780 + fumC Synpcc7942_1007 0.63 -2.3 1,019,782 + fumC Synpcc7942_1007 0.63 -0.2 1,019,788 - fumC Synpcc7942_1007 0.64 +0.2 1,019,796 + fumC Synpcc7942_1007 0.64 -2.7 1,019,799 - fumC Synpcc7942_1007 0.64 +0.2 1,019,926 - fumC Synpcc7942_1007 0.73 -1.7 1,019,936 - fumC Synpcc7942_1007 0.74 -2.1 1,020,150 - fumC Synpcc7942_1007 0.90 -2.3 1,020,203 + +0.6 1,020,219 + -1.4 1,020,219 + -1.3 1,020,252 - +0.6 1,020,280 - -0.8 1,020,338 - +1.7 1,020,348 + +1.0 1,020,365 - -0.6 1,020,422 + purU Synpcc7942_1008 0.11 -1.2 1,020,472 + purU Synpcc7942_1008 0.16 -0.1 1,020,521 + purU Synpcc7942_1008 0.22 -2.8 1,020,528 + purU Synpcc7942_1008 0.23 +0.9 1,020,571 - purU Synpcc7942_1008 0.28 -1.2 1,020,917 - purU Synpcc7942_1008 0.68 -0.5 1,020,955 + purU Synpcc7942_1008 0.73 -0.2 1,020,957 + purU Synpcc7942_1008 0.73 +0.9 1,021,033 - purU Synpcc7942_1008 0.82 -0.6 1,021,033 - purU Synpcc7942_1008 0.82 -1.2 1,021,054 - purU Synpcc7942_1008 0.84 -1.0 1,021,083 + purU Synpcc7942_1008 0.88 -3.0 1,021,152 - -4.7 1,021,157 + -1.0 1,021,227 + +0.6 1,021,268 - -1.9
Or see this region's nucleotide sequence