Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purT and Synpcc7942_0589 are separated by 70 nucleotides Synpcc7942_0589 and Synpcc7942_0590 are separated by 46 nucleotides Synpcc7942_0590 and Synpcc7942_0591 are separated by 4 nucleotides Synpcc7942_0591 and pfkA are separated by 13 nucleotides
Synpcc7942_0588: purT - phosphoribosylglycinamide formyltransferase 2, at 576,665 to 577,840
purT
Synpcc7942_0589: Synpcc7942_0589 - Fe-S-cluster oxidoreductase-like, at 577,911 to 578,573
_0589
Synpcc7942_0590: Synpcc7942_0590 - membrane protein-like, at 578,620 to 578,967
_0590
Synpcc7942_0591: Synpcc7942_0591 - membrane protein-like, at 578,972 to 579,298
_0591
Synpcc7942_0592: pfkA - 6-phosphofructokinase, at 579,312 to 580,394
pfkA
Position (kb) 577
578
579 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 576.948 kb on - strand, within purT at 577.071 kb on - strand, within purT at 577.086 kb on - strand, within purT at 577.136 kb on - strand, within purT at 577.235 kb on - strand, within purT at 577.299 kb on + strand, within purT at 577.316 kb on + strand, within purT at 577.316 kb on + strand, within purT at 577.324 kb on - strand, within purT at 577.326 kb on + strand, within purT at 577.384 kb on - strand, within purT at 577.496 kb on - strand, within purT at 577.599 kb on + strand, within purT at 577.624 kb on - strand, within purT at 577.688 kb on + strand, within purT at 577.789 kb on + strand at 577.789 kb on + strand at 577.797 kb on - strand at 577.797 kb on - strand at 577.797 kb on - strand at 577.862 kb on - strand at 577.881 kb on + strand at 577.934 kb on + strand at 577.936 kb on + strand at 577.938 kb on + strand at 577.939 kb on + strand at 577.939 kb on + strand at 577.944 kb on + strand at 577.944 kb on + strand at 577.947 kb on - strand at 577.951 kb on + strand at 578.063 kb on - strand, within Synpcc7942_0589 at 578.090 kb on - strand, within Synpcc7942_0589 at 578.165 kb on + strand, within Synpcc7942_0589 at 578.173 kb on - strand, within Synpcc7942_0589 at 578.173 kb on - strand, within Synpcc7942_0589 at 578.181 kb on + strand, within Synpcc7942_0589 at 578.181 kb on + strand, within Synpcc7942_0589 at 578.204 kb on - strand, within Synpcc7942_0589 at 578.555 kb on + strand at 578.555 kb on + strand at 578.555 kb on + strand at 578.575 kb on + strand at 578.575 kb on + strand at 578.575 kb on - strand at 578.666 kb on - strand, within Synpcc7942_0590 at 578.679 kb on + strand, within Synpcc7942_0590 at 578.679 kb on + strand, within Synpcc7942_0590 at 578.693 kb on - strand, within Synpcc7942_0590 at 578.759 kb on - strand, within Synpcc7942_0590 at 578.759 kb on - strand, within Synpcc7942_0590 at 578.763 kb on + strand, within Synpcc7942_0590 at 578.787 kb on + strand, within Synpcc7942_0590 at 578.787 kb on + strand, within Synpcc7942_0590 at 578.795 kb on - strand, within Synpcc7942_0590 at 578.797 kb on + strand, within Synpcc7942_0590 at 578.818 kb on + strand, within Synpcc7942_0590 at 578.818 kb on + strand, within Synpcc7942_0590 at 578.826 kb on - strand, within Synpcc7942_0590 at 578.826 kb on - strand, within Synpcc7942_0590 at 578.888 kb on + strand, within Synpcc7942_0590 at 578.888 kb on + strand, within Synpcc7942_0590 at 578.916 kb on + strand, within Synpcc7942_0590 at 578.932 kb on + strand, within Synpcc7942_0590 at 578.932 kb on + strand, within Synpcc7942_0590 at 578.948 kb on + strand at 578.970 kb on - strand at 579.066 kb on + strand, within Synpcc7942_0591 at 579.136 kb on + strand, within Synpcc7942_0591 at 579.137 kb on + strand, within Synpcc7942_0591 at 579.144 kb on - strand, within Synpcc7942_0591 at 579.144 kb on - strand, within Synpcc7942_0591 at 579.158 kb on + strand, within Synpcc7942_0591 at 579.216 kb on + strand, within Synpcc7942_0591 at 579.237 kb on + strand, within Synpcc7942_0591 at 579.285 kb on + strand at 579.292 kb on + strand at 579.292 kb on + strand at 579.293 kb on + strand at 579.294 kb on - strand at 579.294 kb on - strand at 579.300 kb on - strand at 579.304 kb on + strand at 579.326 kb on + strand at 579.328 kb on + strand at 579.331 kb on + strand at 579.354 kb on + strand at 579.358 kb on - strand at 579.387 kb on + strand at 579.394 kb on - strand at 579.411 kb on - strand at 579.419 kb on + strand at 579.427 kb on - strand, within pfkA at 579.427 kb on - strand, within pfkA at 579.427 kb on - strand, within pfkA at 579.476 kb on + strand, within pfkA at 579.483 kb on - strand, within pfkA at 579.513 kb on + strand, within pfkA at 579.525 kb on + strand, within pfkA at 579.531 kb on + strand, within pfkA at 579.544 kb on + strand, within pfkA
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 576,948 - purT Synpcc7942_0588 0.24 -2.3 577,071 - purT Synpcc7942_0588 0.35 -4.5 577,086 - purT Synpcc7942_0588 0.36 -1.8 577,136 - purT Synpcc7942_0588 0.40 -2.3 577,235 - purT Synpcc7942_0588 0.48 -1.9 577,299 + purT Synpcc7942_0588 0.54 -0.8 577,316 + purT Synpcc7942_0588 0.55 -2.8 577,316 + purT Synpcc7942_0588 0.55 -2.8 577,324 - purT Synpcc7942_0588 0.56 -0.7 577,326 + purT Synpcc7942_0588 0.56 -2.4 577,384 - purT Synpcc7942_0588 0.61 -2.7 577,496 - purT Synpcc7942_0588 0.71 -2.1 577,599 + purT Synpcc7942_0588 0.79 -3.1 577,624 - purT Synpcc7942_0588 0.82 -0.3 577,688 + purT Synpcc7942_0588 0.87 -2.3 577,789 + -4.7 577,789 + -3.3 577,797 - -1.0 577,797 - -3.1 577,797 - -2.8 577,862 - +2.0 577,881 + -2.4 577,934 + -0.7 577,936 + -3.1 577,938 + +0.3 577,939 + -2.8 577,939 + -0.9 577,944 + -0.7 577,944 + -1.3 577,947 - -1.4 577,951 + -0.3 578,063 - Synpcc7942_0589 0.23 -2.5 578,090 - Synpcc7942_0589 0.27 -3.2 578,165 + Synpcc7942_0589 0.38 -2.5 578,173 - Synpcc7942_0589 0.40 -0.4 578,173 - Synpcc7942_0589 0.40 -0.3 578,181 + Synpcc7942_0589 0.41 -0.5 578,181 + Synpcc7942_0589 0.41 -0.7 578,204 - Synpcc7942_0589 0.44 -0.2 578,555 + -1.8 578,555 + +1.4 578,555 + +0.6 578,575 + -1.9 578,575 + +0.6 578,575 - -0.1 578,666 - Synpcc7942_0590 0.13 -0.6 578,679 + Synpcc7942_0590 0.17 -0.1 578,679 + Synpcc7942_0590 0.17 -0.4 578,693 - Synpcc7942_0590 0.21 -0.5 578,759 - Synpcc7942_0590 0.40 -2.9 578,759 - Synpcc7942_0590 0.40 +1.0 578,763 + Synpcc7942_0590 0.41 -2.4 578,787 + Synpcc7942_0590 0.48 -1.4 578,787 + Synpcc7942_0590 0.48 -0.6 578,795 - Synpcc7942_0590 0.50 +0.7 578,797 + Synpcc7942_0590 0.51 -0.1 578,818 + Synpcc7942_0590 0.57 -2.5 578,818 + Synpcc7942_0590 0.57 +0.4 578,826 - Synpcc7942_0590 0.59 +0.7 578,826 - Synpcc7942_0590 0.59 -2.7 578,888 + Synpcc7942_0590 0.77 -2.8 578,888 + Synpcc7942_0590 0.77 -2.6 578,916 + Synpcc7942_0590 0.85 +0.7 578,932 + Synpcc7942_0590 0.90 +1.2 578,932 + Synpcc7942_0590 0.90 +0.9 578,948 + -1.7 578,970 - -2.8 579,066 + Synpcc7942_0591 0.29 +1.8 579,136 + Synpcc7942_0591 0.50 +0.7 579,137 + Synpcc7942_0591 0.50 -0.7 579,144 - Synpcc7942_0591 0.53 +1.6 579,144 - Synpcc7942_0591 0.53 +1.6 579,158 + Synpcc7942_0591 0.57 -1.9 579,216 + Synpcc7942_0591 0.75 -1.2 579,237 + Synpcc7942_0591 0.81 -0.1 579,285 + -0.3 579,292 + +0.4 579,292 + -3.2 579,293 + +0.6 579,294 - +0.4 579,294 - +1.5 579,300 - +1.4 579,304 + +0.7 579,326 + +0.9 579,328 + -3.2 579,331 + -2.3 579,354 + +0.8 579,358 - +1.4 579,387 + +0.4 579,394 - +1.1 579,411 - -0.3 579,419 + +0.5 579,427 - pfkA Synpcc7942_0592 0.11 -1.9 579,427 - pfkA Synpcc7942_0592 0.11 +0.3 579,427 - pfkA Synpcc7942_0592 0.11 -0.7 579,476 + pfkA Synpcc7942_0592 0.15 -0.8 579,483 - pfkA Synpcc7942_0592 0.16 -0.2 579,513 + pfkA Synpcc7942_0592 0.19 -0.8 579,525 + pfkA Synpcc7942_0592 0.20 +0.5 579,531 + pfkA Synpcc7942_0592 0.20 -0.3 579,544 + pfkA Synpcc7942_0592 0.21 +1.0
Or see this region's nucleotide sequence