Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recJ and Synpcc7942_0518 are separated by 59 nucleotides Synpcc7942_0518 and Synpcc7942_0519 are separated by 63 nucleotides Synpcc7942_0519 and Synpcc7942_0520 are separated by 49 nucleotides
Synpcc7942_0517: recJ - exonuclease RecJ, at 502,006 to 504,333
recJ
Synpcc7942_0518: Synpcc7942_0518 - hypothetical protein, at 504,393 to 505,556
_0518
Synpcc7942_0519: Synpcc7942_0519 - hypothetical protein, at 505,620 to 506,138
_0519
Synpcc7942_0520: Synpcc7942_0520 - hypothetical protein, at 506,188 to 506,829
_0520
Position (kb) 504
505
506 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 503.396 kb on + strand, within recJ at 503.396 kb on + strand, within recJ at 503.396 kb on + strand, within recJ at 503.399 kb on - strand, within recJ at 503.401 kb on + strand, within recJ at 503.401 kb on + strand, within recJ at 503.402 kb on + strand, within recJ at 503.404 kb on - strand, within recJ at 503.420 kb on + strand, within recJ at 503.422 kb on + strand, within recJ at 503.427 kb on + strand, within recJ at 503.428 kb on - strand, within recJ at 503.428 kb on - strand, within recJ at 503.428 kb on - strand, within recJ at 503.434 kb on + strand, within recJ at 503.447 kb on - strand, within recJ at 503.485 kb on + strand, within recJ at 503.493 kb on - strand, within recJ at 503.518 kb on + strand, within recJ at 503.644 kb on + strand, within recJ at 503.650 kb on + strand at 503.651 kb on - strand, within recJ at 503.651 kb on - strand, within recJ at 503.655 kb on + strand, within recJ at 503.658 kb on - strand, within recJ at 503.673 kb on + strand, within recJ at 503.673 kb on + strand, within recJ at 503.676 kb on - strand, within recJ at 503.685 kb on - strand, within recJ at 503.701 kb on - strand, within recJ at 503.717 kb on - strand, within recJ at 503.732 kb on - strand, within recJ at 503.736 kb on - strand, within recJ at 503.759 kb on + strand, within recJ at 503.767 kb on - strand, within recJ at 503.767 kb on - strand, within recJ at 503.767 kb on - strand, within recJ at 503.767 kb on - strand, within recJ at 503.793 kb on + strand, within recJ at 503.811 kb on - strand, within recJ at 503.831 kb on - strand, within recJ at 503.856 kb on + strand, within recJ at 503.859 kb on - strand, within recJ at 503.865 kb on + strand, within recJ at 503.884 kb on + strand, within recJ at 503.889 kb on + strand, within recJ at 503.897 kb on - strand, within recJ at 503.897 kb on - strand, within recJ at 503.897 kb on - strand, within recJ at 503.911 kb on - strand, within recJ at 503.914 kb on + strand, within recJ at 503.920 kb on + strand, within recJ at 503.920 kb on + strand, within recJ at 503.927 kb on + strand, within recJ at 503.933 kb on + strand, within recJ at 503.935 kb on + strand at 503.935 kb on - strand, within recJ at 503.940 kb on + strand, within recJ at 503.943 kb on - strand, within recJ at 503.952 kb on + strand, within recJ at 503.972 kb on + strand, within recJ at 503.972 kb on + strand, within recJ at 503.980 kb on - strand, within recJ at 504.001 kb on + strand, within recJ at 504.025 kb on - strand, within recJ at 504.075 kb on - strand, within recJ at 504.097 kb on + strand, within recJ at 504.097 kb on + strand, within recJ at 504.106 kb on - strand at 504.144 kb on + strand at 504.152 kb on - strand at 504.152 kb on - strand at 504.152 kb on - strand at 504.152 kb on - strand at 504.162 kb on - strand at 504.166 kb on - strand at 504.173 kb on + strand at 504.213 kb on - strand at 504.240 kb on - strand at 504.250 kb on + strand at 504.264 kb on - strand at 504.266 kb on + strand at 504.276 kb on + strand at 504.312 kb on - strand at 504.330 kb on + strand at 504.330 kb on + strand at 504.349 kb on - strand at 504.349 kb on - strand at 504.349 kb on - strand at 504.351 kb on - strand at 504.353 kb on + strand at 504.361 kb on - strand at 504.397 kb on + strand at 504.409 kb on + strand at 504.409 kb on + strand at 504.545 kb on - strand, within Synpcc7942_0518 at 504.666 kb on + strand, within Synpcc7942_0518 at 504.666 kb on + strand, within Synpcc7942_0518 at 504.704 kb on - strand, within Synpcc7942_0518 at 504.920 kb on - strand, within Synpcc7942_0518 at 504.945 kb on + strand, within Synpcc7942_0518 at 504.945 kb on + strand, within Synpcc7942_0518 at 504.974 kb on - strand, within Synpcc7942_0518 at 505.026 kb on - strand, within Synpcc7942_0518 at 505.066 kb on + strand, within Synpcc7942_0518 at 505.078 kb on + strand, within Synpcc7942_0518 at 505.122 kb on - strand, within Synpcc7942_0518 at 505.187 kb on - strand, within Synpcc7942_0518 at 505.383 kb on + strand, within Synpcc7942_0518 at 505.424 kb on - strand, within Synpcc7942_0518 at 505.480 kb on + strand at 505.480 kb on + strand at 505.488 kb on - strand at 505.548 kb on + strand at 505.548 kb on + strand at 505.550 kb on + strand at 505.556 kb on - strand at 505.568 kb on + strand at 505.576 kb on - strand at 505.576 kb on - strand at 505.579 kb on + strand at 505.601 kb on + strand at 505.607 kb on - strand at 505.609 kb on + strand at 505.632 kb on + strand at 505.680 kb on + strand, within Synpcc7942_0519 at 505.696 kb on - strand, within Synpcc7942_0519 at 505.725 kb on + strand, within Synpcc7942_0519 at 505.781 kb on + strand, within Synpcc7942_0519 at 505.789 kb on - strand, within Synpcc7942_0519 at 505.796 kb on + strand, within Synpcc7942_0519 at 505.796 kb on + strand, within Synpcc7942_0519 at 505.804 kb on - strand, within Synpcc7942_0519 at 505.804 kb on - strand, within Synpcc7942_0519 at 505.804 kb on - strand, within Synpcc7942_0519 at 505.813 kb on - strand, within Synpcc7942_0519 at 505.819 kb on - strand, within Synpcc7942_0519 at 505.829 kb on - strand, within Synpcc7942_0519 at 505.841 kb on - strand, within Synpcc7942_0519 at 505.864 kb on + strand, within Synpcc7942_0519 at 505.883 kb on + strand, within Synpcc7942_0519 at 505.887 kb on - strand, within Synpcc7942_0519 at 505.889 kb on + strand, within Synpcc7942_0519 at 505.916 kb on + strand, within Synpcc7942_0519 at 505.989 kb on + strand, within Synpcc7942_0519 at 505.997 kb on - strand, within Synpcc7942_0519 at 506.004 kb on - strand, within Synpcc7942_0519 at 506.015 kb on + strand, within Synpcc7942_0519 at 506.043 kb on + strand, within Synpcc7942_0519 at 506.099 kb on - strand at 506.099 kb on - strand at 506.099 kb on - strand at 506.111 kb on - strand at 506.111 kb on - strand at 506.139 kb on + strand at 506.185 kb on + strand at 506.194 kb on + strand at 506.271 kb on + strand, within Synpcc7942_0520 at 506.290 kb on - strand, within Synpcc7942_0520 at 506.290 kb on - strand, within Synpcc7942_0520 at 506.291 kb on - strand, within Synpcc7942_0520 at 506.370 kb on - strand, within Synpcc7942_0520 at 506.372 kb on + strand, within Synpcc7942_0520 at 506.372 kb on + strand, within Synpcc7942_0520 at 506.373 kb on + strand, within Synpcc7942_0520 at 506.375 kb on + strand, within Synpcc7942_0520 at 506.380 kb on + strand, within Synpcc7942_0520 at 506.380 kb on - strand, within Synpcc7942_0520 at 506.452 kb on + strand, within Synpcc7942_0520 at 506.470 kb on + strand, within Synpcc7942_0520 at 506.470 kb on + strand, within Synpcc7942_0520 at 506.475 kb on + strand, within Synpcc7942_0520 at 506.478 kb on - strand, within Synpcc7942_0520 at 506.478 kb on - strand, within Synpcc7942_0520 at 506.478 kb on - strand, within Synpcc7942_0520 at 506.479 kb on - strand, within Synpcc7942_0520 at 506.554 kb on + strand, within Synpcc7942_0520
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 503,396 + recJ Synpcc7942_0517 0.60 +0.1 503,396 + recJ Synpcc7942_0517 0.60 +1.2 503,396 + recJ Synpcc7942_0517 0.60 +0.4 503,399 - recJ Synpcc7942_0517 0.60 +0.7 503,401 + recJ Synpcc7942_0517 0.60 +1.2 503,401 + recJ Synpcc7942_0517 0.60 +0.0 503,402 + recJ Synpcc7942_0517 0.60 -0.7 503,404 - recJ Synpcc7942_0517 0.60 -0.3 503,420 + recJ Synpcc7942_0517 0.61 -2.1 503,422 + recJ Synpcc7942_0517 0.61 +0.1 503,427 + recJ Synpcc7942_0517 0.61 -0.1 503,428 - recJ Synpcc7942_0517 0.61 +1.7 503,428 - recJ Synpcc7942_0517 0.61 +0.9 503,428 - recJ Synpcc7942_0517 0.61 +0.8 503,434 + recJ Synpcc7942_0517 0.61 +1.2 503,447 - recJ Synpcc7942_0517 0.62 +0.1 503,485 + recJ Synpcc7942_0517 0.64 -1.2 503,493 - recJ Synpcc7942_0517 0.64 +0.2 503,518 + recJ Synpcc7942_0517 0.65 -1.0 503,644 + recJ Synpcc7942_0517 0.70 -0.5 503,650 + +0.8 503,651 - recJ Synpcc7942_0517 0.71 -1.7 503,651 - recJ Synpcc7942_0517 0.71 +0.7 503,655 + recJ Synpcc7942_0517 0.71 +0.5 503,658 - recJ Synpcc7942_0517 0.71 -0.8 503,673 + recJ Synpcc7942_0517 0.72 +0.4 503,673 + recJ Synpcc7942_0517 0.72 -0.2 503,676 - recJ Synpcc7942_0517 0.72 +0.6 503,685 - recJ Synpcc7942_0517 0.72 +3.0 503,701 - recJ Synpcc7942_0517 0.73 -1.9 503,717 - recJ Synpcc7942_0517 0.73 +0.4 503,732 - recJ Synpcc7942_0517 0.74 -2.9 503,736 - recJ Synpcc7942_0517 0.74 +0.1 503,759 + recJ Synpcc7942_0517 0.75 +0.4 503,767 - recJ Synpcc7942_0517 0.76 +1.6 503,767 - recJ Synpcc7942_0517 0.76 +0.2 503,767 - recJ Synpcc7942_0517 0.76 -0.3 503,767 - recJ Synpcc7942_0517 0.76 -1.4 503,793 + recJ Synpcc7942_0517 0.77 -0.5 503,811 - recJ Synpcc7942_0517 0.78 +0.3 503,831 - recJ Synpcc7942_0517 0.78 -0.5 503,856 + recJ Synpcc7942_0517 0.79 -3.4 503,859 - recJ Synpcc7942_0517 0.80 -1.1 503,865 + recJ Synpcc7942_0517 0.80 -0.8 503,884 + recJ Synpcc7942_0517 0.81 +0.5 503,889 + recJ Synpcc7942_0517 0.81 +0.5 503,897 - recJ Synpcc7942_0517 0.81 +0.7 503,897 - recJ Synpcc7942_0517 0.81 +0.6 503,897 - recJ Synpcc7942_0517 0.81 +1.0 503,911 - recJ Synpcc7942_0517 0.82 -0.5 503,914 + recJ Synpcc7942_0517 0.82 -1.0 503,920 + recJ Synpcc7942_0517 0.82 -2.3 503,920 + recJ Synpcc7942_0517 0.82 +0.7 503,927 + recJ Synpcc7942_0517 0.83 -2.3 503,933 + recJ Synpcc7942_0517 0.83 +0.5 503,935 + +0.2 503,935 - recJ Synpcc7942_0517 0.83 +0.0 503,940 + recJ Synpcc7942_0517 0.83 -2.8 503,943 - recJ Synpcc7942_0517 0.83 +0.7 503,952 + recJ Synpcc7942_0517 0.84 -2.1 503,972 + recJ Synpcc7942_0517 0.84 -3.0 503,972 + recJ Synpcc7942_0517 0.84 +0.6 503,980 - recJ Synpcc7942_0517 0.85 -0.2 504,001 + recJ Synpcc7942_0517 0.86 +0.3 504,025 - recJ Synpcc7942_0517 0.87 -1.0 504,075 - recJ Synpcc7942_0517 0.89 +0.5 504,097 + recJ Synpcc7942_0517 0.90 -1.8 504,097 + recJ Synpcc7942_0517 0.90 -3.5 504,106 - +2.3 504,144 + -0.8 504,152 - +0.2 504,152 - +0.9 504,152 - +1.4 504,152 - +0.3 504,162 - -4.0 504,166 - -0.7 504,173 + +0.6 504,213 - +0.1 504,240 - -0.1 504,250 + +0.1 504,264 - -1.1 504,266 + -1.0 504,276 + -0.8 504,312 - +0.9 504,330 + -3.1 504,330 + -0.6 504,349 - -0.2 504,349 - +1.6 504,349 - +0.1 504,351 - +3.1 504,353 + +0.5 504,361 - +0.2 504,397 + -3.9 504,409 + -0.1 504,409 + -4.6 504,545 - Synpcc7942_0518 0.13 -2.5 504,666 + Synpcc7942_0518 0.23 -1.6 504,666 + Synpcc7942_0518 0.23 -2.1 504,704 - Synpcc7942_0518 0.27 -3.6 504,920 - Synpcc7942_0518 0.45 -2.5 504,945 + Synpcc7942_0518 0.47 -1.0 504,945 + Synpcc7942_0518 0.47 -0.8 504,974 - Synpcc7942_0518 0.50 -5.5 505,026 - Synpcc7942_0518 0.54 -4.1 505,066 + Synpcc7942_0518 0.58 -2.3 505,078 + Synpcc7942_0518 0.59 -2.4 505,122 - Synpcc7942_0518 0.63 -2.4 505,187 - Synpcc7942_0518 0.68 -1.4 505,383 + Synpcc7942_0518 0.85 -2.8 505,424 - Synpcc7942_0518 0.89 -3.5 505,480 + -0.7 505,480 + +1.9 505,488 - -1.8 505,548 + -0.8 505,548 + -3.5 505,550 + +0.0 505,556 - -0.3 505,568 + +0.2 505,576 - +1.4 505,576 - -0.3 505,579 + +0.7 505,601 + -1.4 505,607 - +0.8 505,609 + -2.3 505,632 + +0.4 505,680 + Synpcc7942_0519 0.12 -1.2 505,696 - Synpcc7942_0519 0.15 -2.5 505,725 + Synpcc7942_0519 0.20 +2.8 505,781 + Synpcc7942_0519 0.31 -1.3 505,789 - Synpcc7942_0519 0.33 -1.5 505,796 + Synpcc7942_0519 0.34 -3.4 505,796 + Synpcc7942_0519 0.34 -0.4 505,804 - Synpcc7942_0519 0.35 +0.2 505,804 - Synpcc7942_0519 0.35 +0.9 505,804 - Synpcc7942_0519 0.35 +1.2 505,813 - Synpcc7942_0519 0.37 -0.3 505,819 - Synpcc7942_0519 0.38 +0.4 505,829 - Synpcc7942_0519 0.40 -2.2 505,841 - Synpcc7942_0519 0.43 -0.7 505,864 + Synpcc7942_0519 0.47 -0.3 505,883 + Synpcc7942_0519 0.51 -0.3 505,887 - Synpcc7942_0519 0.51 +1.9 505,889 + Synpcc7942_0519 0.52 +0.1 505,916 + Synpcc7942_0519 0.57 -3.8 505,989 + Synpcc7942_0519 0.71 +1.2 505,997 - Synpcc7942_0519 0.73 +1.2 506,004 - Synpcc7942_0519 0.74 +0.4 506,015 + Synpcc7942_0519 0.76 +0.9 506,043 + Synpcc7942_0519 0.82 +0.3 506,099 - +2.2 506,099 - -1.1 506,099 - -1.7 506,111 - +1.3 506,111 - -1.2 506,139 + +1.0 506,185 + +0.2 506,194 + +0.1 506,271 + Synpcc7942_0520 0.13 -0.7 506,290 - Synpcc7942_0520 0.16 +1.7 506,290 - Synpcc7942_0520 0.16 +0.7 506,291 - Synpcc7942_0520 0.16 -2.7 506,370 - Synpcc7942_0520 0.28 +1.1 506,372 + Synpcc7942_0520 0.29 -1.4 506,372 + Synpcc7942_0520 0.29 -0.7 506,373 + Synpcc7942_0520 0.29 +0.6 506,375 + Synpcc7942_0520 0.29 -2.1 506,380 + Synpcc7942_0520 0.30 +0.1 506,380 - Synpcc7942_0520 0.30 +0.0 506,452 + Synpcc7942_0520 0.41 -0.5 506,470 + Synpcc7942_0520 0.44 -1.7 506,470 + Synpcc7942_0520 0.44 +0.7 506,475 + Synpcc7942_0520 0.45 -2.4 506,478 - Synpcc7942_0520 0.45 +0.2 506,478 - Synpcc7942_0520 0.45 -0.7 506,478 - Synpcc7942_0520 0.45 +0.7 506,479 - Synpcc7942_0520 0.45 +0.9 506,554 + Synpcc7942_0520 0.57 +0.6
Or see this region's nucleotide sequence