Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmK3 and ccmK4 are separated by 44 nucleotides ccmK4 and Synpcc7942_0286 are separated by 84 nucleotides Synpcc7942_0286 and Synpcc7942_0287 are separated by 54 nucleotides Synpcc7942_0287 and glmU are separated by 11 nucleotides
Synpcc7942_0284: ccmK3 - carbon dioxide concentrating mechanism protein CcmK, at 280,701 to 281,009
ccmK3
Synpcc7942_0285: ccmK4 - carbon dioxide concentrating mechanism protein CcmK, at 281,054 to 281,395
ccmK4
Synpcc7942_0286: Synpcc7942_0286 - hypothetical protein, at 281,480 to 281,842
_0286
Synpcc7942_0287: Synpcc7942_0287 - hypothetical protein, at 281,897 to 282,592
_0287
Synpcc7942_0288: glmU - glucosamine-1-phosphate N-acetyltransferase / UDP-N-acetylglucosamine pyrophosphorylase, at 282,604 to 283,962
glmU
Position (kb) 281
282
283 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 280.968 kb on - strand, within ccmK3 at 281.396 kb on + strand at 281.396 kb on + strand at 281.449 kb on + strand at 281.506 kb on + strand at 281.567 kb on + strand, within Synpcc7942_0286 at 281.567 kb on + strand, within Synpcc7942_0286 at 281.630 kb on - strand, within Synpcc7942_0286 at 281.633 kb on + strand, within Synpcc7942_0286 at 281.646 kb on - strand, within Synpcc7942_0286 at 281.725 kb on + strand, within Synpcc7942_0286 at 281.785 kb on - strand, within Synpcc7942_0286 at 281.818 kb on - strand at 281.861 kb on - strand at 281.872 kb on + strand at 281.887 kb on - strand at 281.894 kb on + strand at 281.894 kb on + strand at 281.908 kb on + strand at 281.908 kb on - strand at 281.909 kb on - strand at 281.916 kb on - strand at 281.919 kb on + strand at 281.955 kb on - strand at 281.965 kb on - strand at 281.968 kb on + strand, within Synpcc7942_0287 at 282.012 kb on + strand, within Synpcc7942_0287 at 282.020 kb on - strand, within Synpcc7942_0287 at 282.340 kb on - strand, within Synpcc7942_0287 at 282.454 kb on + strand, within Synpcc7942_0287 at 282.610 kb on - strand at 282.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 280,968 - ccmK3 Synpcc7942_0284 0.86 -2.2 281,396 + -0.8 281,396 + +0.4 281,449 + +0.6 281,506 + +0.7 281,567 + Synpcc7942_0286 0.24 -0.2 281,567 + Synpcc7942_0286 0.24 -0.3 281,630 - Synpcc7942_0286 0.41 +0.1 281,633 + Synpcc7942_0286 0.42 +0.0 281,646 - Synpcc7942_0286 0.46 +0.2 281,725 + Synpcc7942_0286 0.67 +0.5 281,785 - Synpcc7942_0286 0.84 +0.3 281,818 - +0.0 281,861 - +0.4 281,872 + +1.5 281,887 - +1.8 281,894 + +0.5 281,894 + -0.2 281,908 + -0.4 281,908 - +1.0 281,909 - -1.0 281,916 - -1.3 281,919 + -2.8 281,955 - -0.9 281,965 - -0.8 281,968 + Synpcc7942_0287 0.10 -0.6 282,012 + Synpcc7942_0287 0.17 -0.9 282,020 - Synpcc7942_0287 0.18 -2.6 282,340 - Synpcc7942_0287 0.64 -0.9 282,454 + Synpcc7942_0287 0.80 -1.7 282,610 - +0.9 282,621 - +0.2
Or see this region's nucleotide sequence