Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdhB and Synpcc7942_0144 are separated by 284 nucleotides Synpcc7942_0144 and Synpcc7942_0145 overlap by 4 nucleotides Synpcc7942_0145 and Synpcc7942_0146 are separated by 119 nucleotides
Synpcc7942_0143: pdhB - pyruvate/2-oxoglutarate dehydrogenase complex dehydrogenase (E1) component, at 145,003 to 145,983
pdhB
Synpcc7942_0144: Synpcc7942_0144 - hypothetical protein, at 146,268 to 146,720
_0144
Synpcc7942_0145: Synpcc7942_0145 - hypothetical protein, at 146,717 to 147,040
_0145
Synpcc7942_0146: Synpcc7942_0146 - hypothetical protein, at 147,160 to 147,855
_0146
Position (kb)
146
147
148 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 146.067 kb on - strand at 146.080 kb on - strand at 146.095 kb on + strand at 146.096 kb on - strand at 146.098 kb on + strand at 146.103 kb on - strand at 146.151 kb on + strand at 146.153 kb on - strand at 146.154 kb on - strand at 146.159 kb on - strand at 146.159 kb on - strand at 146.161 kb on + strand at 146.161 kb on + strand at 146.161 kb on + strand at 146.175 kb on - strand at 146.186 kb on - strand at 146.241 kb on - strand at 146.241 kb on - strand at 146.251 kb on + strand at 146.252 kb on + strand at 146.252 kb on + strand at 146.252 kb on + strand at 146.252 kb on + strand at 146.252 kb on + strand at 146.260 kb on - strand at 146.260 kb on - strand at 146.260 kb on - strand at 146.271 kb on - strand at 146.349 kb on + strand, within Synpcc7942_0144 at 146.356 kb on - strand, within Synpcc7942_0144 at 146.389 kb on - strand at 146.391 kb on + strand, within Synpcc7942_0144 at 146.411 kb on - strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.451 kb on + strand, within Synpcc7942_0144 at 146.459 kb on - strand, within Synpcc7942_0144 at 146.459 kb on - strand, within Synpcc7942_0144 at 146.459 kb on - strand at 146.459 kb on - strand, within Synpcc7942_0144 at 146.459 kb on - strand, within Synpcc7942_0144 at 146.459 kb on - strand, within Synpcc7942_0144 at 146.461 kb on + strand, within Synpcc7942_0144 at 146.462 kb on + strand, within Synpcc7942_0144 at 146.486 kb on + strand, within Synpcc7942_0144 at 146.486 kb on + strand, within Synpcc7942_0144 at 146.486 kb on + strand, within Synpcc7942_0144 at 146.486 kb on + strand, within Synpcc7942_0144 at 146.494 kb on - strand, within Synpcc7942_0144 at 146.494 kb on - strand, within Synpcc7942_0144 at 146.497 kb on + strand, within Synpcc7942_0144 at 146.507 kb on + strand, within Synpcc7942_0144 at 146.536 kb on + strand, within Synpcc7942_0144 at 146.573 kb on - strand, within Synpcc7942_0144 at 146.616 kb on - strand, within Synpcc7942_0144 at 146.616 kb on - strand, within Synpcc7942_0144 at 146.688 kb on - strand at 146.728 kb on - strand at 146.728 kb on - strand at 146.758 kb on + strand, within Synpcc7942_0145 at 147.020 kb on + strand at 147.103 kb on + strand at 147.103 kb on + strand at 147.103 kb on + strand at 147.103 kb on + strand at 147.111 kb on - strand at 147.111 kb on - strand at 147.111 kb on - strand at 147.111 kb on - strand at 147.111 kb on - strand at 147.111 kb on - strand at 147.120 kb on - strand at 147.166 kb on + strand at 147.166 kb on + strand at 147.171 kb on + strand at 147.186 kb on - strand at 147.191 kb on - strand at 147.214 kb on + strand at 147.214 kb on + strand at 147.215 kb on - strand at 147.220 kb on + strand at 147.221 kb on + strand at 147.224 kb on - strand at 147.224 kb on - strand at 147.244 kb on + strand, within Synpcc7942_0146 at 147.262 kb on + strand, within Synpcc7942_0146 at 147.383 kb on + strand, within Synpcc7942_0146 at 147.402 kb on - strand, within Synpcc7942_0146 at 147.430 kb on + strand, within Synpcc7942_0146 at 147.430 kb on + strand, within Synpcc7942_0146 at 147.440 kb on + strand, within Synpcc7942_0146 at 147.440 kb on + strand, within Synpcc7942_0146 at 147.440 kb on + strand, within Synpcc7942_0146 at 147.448 kb on - strand, within Synpcc7942_0146 at 147.463 kb on + strand, within Synpcc7942_0146 at 147.463 kb on + strand, within Synpcc7942_0146 at 147.478 kb on + strand, within Synpcc7942_0146 at 147.501 kb on + strand, within Synpcc7942_0146 at 147.501 kb on + strand, within Synpcc7942_0146 at 147.534 kb on + strand, within Synpcc7942_0146 at 147.549 kb on - strand, within Synpcc7942_0146 at 147.554 kb on + strand, within Synpcc7942_0146 at 147.554 kb on + strand, within Synpcc7942_0146 at 147.559 kb on - strand, within Synpcc7942_0146 at 147.631 kb on + strand, within Synpcc7942_0146 at 147.634 kb on + strand at 147.636 kb on + strand, within Synpcc7942_0146 at 147.641 kb on + strand, within Synpcc7942_0146 at 147.643 kb on - strand, within Synpcc7942_0146 at 147.654 kb on + strand, within Synpcc7942_0146 at 147.655 kb on + strand, within Synpcc7942_0146 at 147.681 kb on + strand, within Synpcc7942_0146 at 147.688 kb on + strand, within Synpcc7942_0146 at 147.688 kb on + strand, within Synpcc7942_0146 at 147.706 kb on - strand, within Synpcc7942_0146 at 147.726 kb on + strand, within Synpcc7942_0146 at 147.726 kb on + strand, within Synpcc7942_0146 at 147.759 kb on + strand, within Synpcc7942_0146 at 147.759 kb on + strand, within Synpcc7942_0146 at 147.767 kb on - strand, within Synpcc7942_0146 at 147.770 kb on + strand, within Synpcc7942_0146 at 147.770 kb on + strand, within Synpcc7942_0146 at 147.770 kb on + strand, within Synpcc7942_0146 at 147.770 kb on + strand, within Synpcc7942_0146 at 147.778 kb on + strand, within Synpcc7942_0146 at 147.778 kb on - strand, within Synpcc7942_0146 at 147.778 kb on - strand, within Synpcc7942_0146 at 147.793 kb on + strand at 147.793 kb on + strand at 147.795 kb on - strand at 147.802 kb on - strand at 147.820 kb on + strand at 147.838 kb on + strand at 147.838 kb on + strand at 147.846 kb on - strand at 147.858 kb on - strand at 147.858 kb on - strand at 147.877 kb on - strand at 147.888 kb on + strand at 147.888 kb on + strand at 147.896 kb on - strand at 147.896 kb on - strand at 147.896 kb on - strand at 147.896 kb on - strand at 147.896 kb on - strand at 147.897 kb on - strand at 147.925 kb on - strand at 147.938 kb on + strand at 147.940 kb on + strand at 147.955 kb on + strand at 147.955 kb on + strand at 147.955 kb on + strand at 147.957 kb on + strand at 147.965 kb on - strand at 147.981 kb on - strand at 148.016 kb on - strand at 148.032 kb on + strand at 148.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 146,067 - +2.1 146,080 - -3.1 146,095 + +1.7 146,096 - -0.7 146,098 + +0.2 146,103 - +0.4 146,151 + -0.9 146,153 - +0.5 146,154 - -1.2 146,159 - -0.6 146,159 - +0.6 146,161 + +1.6 146,161 + -0.8 146,161 + +0.7 146,175 - +1.9 146,186 - +0.6 146,241 - +1.1 146,241 - +0.8 146,251 + +0.5 146,252 + -0.4 146,252 + +0.6 146,252 + -1.8 146,252 + -0.4 146,252 + -3.4 146,260 - -3.1 146,260 - +0.5 146,260 - +1.1 146,271 - +0.6 146,349 + Synpcc7942_0144 0.18 -0.1 146,356 - Synpcc7942_0144 0.19 -1.1 146,389 - +0.4 146,391 + Synpcc7942_0144 0.27 +0.3 146,411 - Synpcc7942_0144 0.32 +1.6 146,451 + Synpcc7942_0144 0.40 -0.1 146,451 + Synpcc7942_0144 0.40 -0.6 146,451 + Synpcc7942_0144 0.40 -2.4 146,451 + Synpcc7942_0144 0.40 -3.8 146,451 + Synpcc7942_0144 0.40 -0.8 146,451 + Synpcc7942_0144 0.40 -0.8 146,451 + Synpcc7942_0144 0.40 +0.5 146,451 + Synpcc7942_0144 0.40 +0.2 146,451 + Synpcc7942_0144 0.40 +0.1 146,459 - Synpcc7942_0144 0.42 +0.0 146,459 - Synpcc7942_0144 0.42 +0.8 146,459 - -0.6 146,459 - Synpcc7942_0144 0.42 -0.0 146,459 - Synpcc7942_0144 0.42 +1.1 146,459 - Synpcc7942_0144 0.42 -2.5 146,461 + Synpcc7942_0144 0.43 +1.5 146,462 + Synpcc7942_0144 0.43 +0.8 146,486 + Synpcc7942_0144 0.48 +0.8 146,486 + Synpcc7942_0144 0.48 +0.4 146,486 + Synpcc7942_0144 0.48 -1.3 146,486 + Synpcc7942_0144 0.48 -1.0 146,494 - Synpcc7942_0144 0.50 -3.1 146,494 - Synpcc7942_0144 0.50 +0.5 146,497 + Synpcc7942_0144 0.51 +0.1 146,507 + Synpcc7942_0144 0.53 -2.5 146,536 + Synpcc7942_0144 0.59 +0.6 146,573 - Synpcc7942_0144 0.67 -2.6 146,616 - Synpcc7942_0144 0.77 +0.3 146,616 - Synpcc7942_0144 0.77 +0.1 146,688 - -0.0 146,728 - -2.1 146,728 - -3.4 146,758 + Synpcc7942_0145 0.13 -0.6 147,020 + -2.4 147,103 + -0.3 147,103 + +1.2 147,103 + -0.1 147,103 + +1.1 147,111 - +0.1 147,111 - -0.3 147,111 - +0.0 147,111 - +1.4 147,111 - +0.4 147,111 - +2.4 147,120 - +1.2 147,166 + -0.1 147,166 + -0.4 147,171 + -0.1 147,186 - +0.1 147,191 - -2.8 147,214 + -0.2 147,214 + +0.4 147,215 - +1.4 147,220 + +0.8 147,221 + +0.1 147,224 - -3.0 147,224 - +1.2 147,244 + Synpcc7942_0146 0.12 -3.2 147,262 + Synpcc7942_0146 0.15 -0.1 147,383 + Synpcc7942_0146 0.32 +0.2 147,402 - Synpcc7942_0146 0.35 +0.7 147,430 + Synpcc7942_0146 0.39 +0.4 147,430 + Synpcc7942_0146 0.39 +1.3 147,440 + Synpcc7942_0146 0.40 -0.5 147,440 + Synpcc7942_0146 0.40 +0.3 147,440 + Synpcc7942_0146 0.40 +1.0 147,448 - Synpcc7942_0146 0.41 -0.9 147,463 + Synpcc7942_0146 0.44 +0.8 147,463 + Synpcc7942_0146 0.44 -1.2 147,478 + Synpcc7942_0146 0.46 +1.1 147,501 + Synpcc7942_0146 0.49 +0.3 147,501 + Synpcc7942_0146 0.49 +0.2 147,534 + Synpcc7942_0146 0.54 +2.3 147,549 - Synpcc7942_0146 0.56 +0.0 147,554 + Synpcc7942_0146 0.57 -0.2 147,554 + Synpcc7942_0146 0.57 -3.1 147,559 - Synpcc7942_0146 0.57 -0.5 147,631 + Synpcc7942_0146 0.68 -1.7 147,634 + +0.4 147,636 + Synpcc7942_0146 0.68 +0.3 147,641 + Synpcc7942_0146 0.69 -1.6 147,643 - Synpcc7942_0146 0.69 -1.9 147,654 + Synpcc7942_0146 0.71 -3.0 147,655 + Synpcc7942_0146 0.71 +1.0 147,681 + Synpcc7942_0146 0.75 -1.2 147,688 + Synpcc7942_0146 0.76 +0.6 147,688 + Synpcc7942_0146 0.76 -1.2 147,706 - Synpcc7942_0146 0.78 -0.2 147,726 + Synpcc7942_0146 0.81 -0.2 147,726 + Synpcc7942_0146 0.81 +1.0 147,759 + Synpcc7942_0146 0.86 +0.9 147,759 + Synpcc7942_0146 0.86 +0.4 147,767 - Synpcc7942_0146 0.87 +0.2 147,770 + Synpcc7942_0146 0.88 +0.4 147,770 + Synpcc7942_0146 0.88 -1.1 147,770 + Synpcc7942_0146 0.88 -1.1 147,770 + Synpcc7942_0146 0.88 +0.6 147,778 + Synpcc7942_0146 0.89 +0.6 147,778 - Synpcc7942_0146 0.89 -3.0 147,778 - Synpcc7942_0146 0.89 +1.3 147,793 + -4.1 147,793 + +1.4 147,795 - -3.4 147,802 - +0.6 147,820 + +0.2 147,838 + -0.1 147,838 + +0.8 147,846 - -1.3 147,858 - -1.4 147,858 - +0.4 147,877 - -0.2 147,888 + -1.0 147,888 + +0.6 147,896 - +0.1 147,896 - -1.6 147,896 - +0.5 147,896 - +0.1 147,896 - -0.8 147,897 - -0.3 147,925 - +0.0 147,938 + -0.7 147,940 + +0.5 147,955 + +1.2 147,955 + +0.1 147,955 + -0.2 147,957 + +2.4 147,965 - -0.3 147,981 - -0.7 148,016 - -0.4 148,032 + +0.5 148,040 - -0.6
Or see this region's nucleotide sequence