Experiment: plate grown in LD 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbC and Synpcc7942_0059 are separated by 20 nucleotides Synpcc7942_0059 and Synpcc7942_0060 overlap by 22 nucleotides Synpcc7942_0060 and rfbG overlap by 4 nucleotides rfbG and rfbF overlap by 4 nucleotides
Synpcc7942_0058: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 60,301 to 60,846
rfbC
Synpcc7942_0059: Synpcc7942_0059 - hypothetical protein, at 60,867 to 61,112
_0059
Synpcc7942_0060: Synpcc7942_0060 - hypothetical protein, at 61,091 to 61,369
_0060
Synpcc7942_0061: rfbG - CDP-glucose 4,6-dehydratase, at 61,366 to 62,472
rfbG
Synpcc7942_0062: rfbF - glucose-1-phosphate cytidylyltransferase, at 62,469 to 63,242
rfbF
Position (kb) 61
62
63 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 60.378 kb on + strand, within rfbC at 60.386 kb on - strand, within rfbC at 60.396 kb on - strand, within rfbC at 60.436 kb on - strand, within rfbC at 60.468 kb on + strand, within rfbC at 60.476 kb on - strand, within rfbC at 60.496 kb on + strand, within rfbC at 60.506 kb on + strand, within rfbC at 60.512 kb on - strand, within rfbC at 60.514 kb on - strand, within rfbC at 60.514 kb on - strand, within rfbC at 60.514 kb on - strand, within rfbC at 60.516 kb on + strand, within rfbC at 60.555 kb on - strand, within rfbC at 60.573 kb on + strand, within rfbC at 60.587 kb on + strand, within rfbC at 60.587 kb on + strand, within rfbC at 60.589 kb on - strand, within rfbC at 60.595 kb on - strand, within rfbC at 60.595 kb on - strand, within rfbC at 60.621 kb on + strand, within rfbC at 60.629 kb on - strand, within rfbC at 60.666 kb on + strand, within rfbC at 60.682 kb on - strand, within rfbC at 60.688 kb on - strand, within rfbC at 60.696 kb on - strand, within rfbC at 60.704 kb on + strand, within rfbC at 60.712 kb on - strand, within rfbC at 60.716 kb on + strand, within rfbC at 60.724 kb on - strand, within rfbC at 60.724 kb on - strand, within rfbC at 60.724 kb on - strand, within rfbC at 60.813 kb on - strand at 60.818 kb on - strand at 60.835 kb on - strand at 60.953 kb on + strand, within Synpcc7942_0059 at 60.961 kb on - strand, within Synpcc7942_0059 at 60.975 kb on - strand, within Synpcc7942_0059 at 60.996 kb on - strand, within Synpcc7942_0059 at 61.050 kb on - strand, within Synpcc7942_0059 at 61.058 kb on - strand, within Synpcc7942_0059 at 61.083 kb on - strand, within Synpcc7942_0059 at 61.119 kb on + strand, within Synpcc7942_0060 at 61.208 kb on - strand, within Synpcc7942_0060 at 61.225 kb on + strand, within Synpcc7942_0060 at 61.270 kb on - strand, within Synpcc7942_0060 at 61.282 kb on + strand, within Synpcc7942_0060 at 61.290 kb on - strand, within Synpcc7942_0060 at 61.295 kb on - strand, within Synpcc7942_0060 at 61.317 kb on + strand, within Synpcc7942_0060 at 61.361 kb on - strand at 61.362 kb on - strand at 61.371 kb on - strand at 61.387 kb on + strand at 61.388 kb on - strand at 61.389 kb on + strand at 61.395 kb on - strand at 61.400 kb on + strand at 61.431 kb on - strand at 61.442 kb on - strand at 61.459 kb on - strand at 61.492 kb on + strand, within rfbG at 61.492 kb on + strand, within rfbG at 61.498 kb on + strand, within rfbG at 61.541 kb on - strand, within rfbG at 61.543 kb on + strand, within rfbG at 61.548 kb on + strand, within rfbG at 61.578 kb on + strand, within rfbG at 61.578 kb on + strand, within rfbG at 61.586 kb on - strand, within rfbG at 61.599 kb on - strand, within rfbG at 61.601 kb on + strand, within rfbG at 61.609 kb on - strand, within rfbG at 61.634 kb on - strand, within rfbG at 61.635 kb on + strand, within rfbG at 61.658 kb on - strand, within rfbG at 61.667 kb on + strand, within rfbG at 61.693 kb on + strand, within rfbG at 61.703 kb on - strand, within rfbG at 61.723 kb on + strand, within rfbG at 61.733 kb on + strand, within rfbG at 61.733 kb on - strand, within rfbG at 61.764 kb on + strand, within rfbG at 61.764 kb on + strand, within rfbG at 61.812 kb on - strand, within rfbG at 61.812 kb on - strand, within rfbG at 61.814 kb on + strand, within rfbG at 61.845 kb on - strand, within rfbG at 61.847 kb on + strand, within rfbG at 61.850 kb on + strand, within rfbG at 61.855 kb on - strand, within rfbG at 61.855 kb on - strand, within rfbG at 61.954 kb on + strand, within rfbG at 61.960 kb on + strand, within rfbG at 61.960 kb on + strand, within rfbG at 61.970 kb on + strand, within rfbG at 61.970 kb on + strand, within rfbG at 61.978 kb on - strand, within rfbG at 61.982 kb on + strand, within rfbG at 61.984 kb on + strand, within rfbG at 61.984 kb on + strand, within rfbG at 61.990 kb on + strand, within rfbG at 61.990 kb on - strand, within rfbG at 61.990 kb on - strand, within rfbG at 61.992 kb on - strand, within rfbG at 62.025 kb on + strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.060 kb on - strand, within rfbG at 62.060 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.096 kb on + strand, within rfbG at 62.115 kb on + strand, within rfbG at 62.130 kb on + strand, within rfbG at 62.147 kb on + strand, within rfbG at 62.170 kb on - strand, within rfbG at 62.176 kb on + strand, within rfbG at 62.184 kb on - strand, within rfbG at 62.184 kb on - strand, within rfbG at 62.195 kb on + strand, within rfbG at 62.203 kb on - strand, within rfbG at 62.205 kb on - strand, within rfbG at 62.205 kb on - strand, within rfbG at 62.231 kb on - strand, within rfbG at 62.236 kb on + strand, within rfbG at 62.249 kb on + strand, within rfbG at 62.284 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.322 kb on - strand, within rfbG at 62.329 kb on - strand, within rfbG at 62.338 kb on + strand, within rfbG at 62.355 kb on + strand, within rfbG at 62.378 kb on - strand at 62.378 kb on - strand at 62.434 kb on + strand at 62.434 kb on + strand at 62.434 kb on + strand at 62.440 kb on - strand at 62.440 kb on - strand at 62.440 kb on - strand at 62.442 kb on - strand at 62.442 kb on - strand at 62.442 kb on - strand at 62.450 kb on - strand at 62.459 kb on + strand at 62.480 kb on + strand at 62.488 kb on - strand at 62.488 kb on - strand at 62.503 kb on + strand at 62.511 kb on - strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.522 kb on - strand at 62.531 kb on + strand at 62.547 kb on - strand, within rfbF at 62.578 kb on - strand, within rfbF at 62.588 kb on - strand, within rfbF at 62.740 kb on - strand, within rfbF at 62.741 kb on + strand, within rfbF at 62.753 kb on + strand, within rfbF at 62.755 kb on - strand, within rfbF at 62.756 kb on - strand, within rfbF at 62.939 kb on - strand, within rfbF at 62.964 kb on - strand, within rfbF at 63.181 kb on - strand at 63.286 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction plate grown in LD 12:12 remove 60,378 + rfbC Synpcc7942_0058 0.14 +0.6 60,386 - rfbC Synpcc7942_0058 0.16 -1.9 60,396 - rfbC Synpcc7942_0058 0.17 +0.0 60,436 - rfbC Synpcc7942_0058 0.25 -1.9 60,468 + rfbC Synpcc7942_0058 0.31 -0.2 60,476 - rfbC Synpcc7942_0058 0.32 +0.2 60,496 + rfbC Synpcc7942_0058 0.36 -1.6 60,506 + rfbC Synpcc7942_0058 0.38 -3.1 60,512 - rfbC Synpcc7942_0058 0.39 +2.4 60,514 - rfbC Synpcc7942_0058 0.39 +0.3 60,514 - rfbC Synpcc7942_0058 0.39 +1.8 60,514 - rfbC Synpcc7942_0058 0.39 +0.0 60,516 + rfbC Synpcc7942_0058 0.39 +0.7 60,555 - rfbC Synpcc7942_0058 0.47 +0.4 60,573 + rfbC Synpcc7942_0058 0.50 -4.7 60,587 + rfbC Synpcc7942_0058 0.52 +0.5 60,587 + rfbC Synpcc7942_0058 0.52 -0.3 60,589 - rfbC Synpcc7942_0058 0.53 +0.5 60,595 - rfbC Synpcc7942_0058 0.54 -0.0 60,595 - rfbC Synpcc7942_0058 0.54 +0.7 60,621 + rfbC Synpcc7942_0058 0.59 +0.5 60,629 - rfbC Synpcc7942_0058 0.60 -0.1 60,666 + rfbC Synpcc7942_0058 0.67 -3.4 60,682 - rfbC Synpcc7942_0058 0.70 +0.5 60,688 - rfbC Synpcc7942_0058 0.71 +0.7 60,696 - rfbC Synpcc7942_0058 0.72 +0.2 60,704 + rfbC Synpcc7942_0058 0.74 -0.4 60,712 - rfbC Synpcc7942_0058 0.75 +0.9 60,716 + rfbC Synpcc7942_0058 0.76 +0.2 60,724 - rfbC Synpcc7942_0058 0.77 +0.9 60,724 - rfbC Synpcc7942_0058 0.77 +1.0 60,724 - rfbC Synpcc7942_0058 0.77 +0.3 60,813 - -0.7 60,818 - -2.5 60,835 - +0.4 60,953 + Synpcc7942_0059 0.35 -0.1 60,961 - Synpcc7942_0059 0.38 +1.7 60,975 - Synpcc7942_0059 0.44 +0.9 60,996 - Synpcc7942_0059 0.52 +0.2 61,050 - Synpcc7942_0059 0.74 -0.2 61,058 - Synpcc7942_0059 0.78 +0.4 61,083 - Synpcc7942_0059 0.88 +0.4 61,119 + Synpcc7942_0060 0.10 -0.7 61,208 - Synpcc7942_0060 0.42 -1.1 61,225 + Synpcc7942_0060 0.48 -0.8 61,270 - Synpcc7942_0060 0.64 -2.0 61,282 + Synpcc7942_0060 0.68 +0.8 61,290 - Synpcc7942_0060 0.71 +0.5 61,295 - Synpcc7942_0060 0.73 +0.2 61,317 + Synpcc7942_0060 0.81 +1.7 61,361 - +1.1 61,362 - +0.7 61,371 - -2.5 61,387 + +0.3 61,388 - +2.1 61,389 + -1.1 61,395 - -1.1 61,400 + -0.1 61,431 - +0.7 61,442 - -1.1 61,459 - +1.4 61,492 + rfbG Synpcc7942_0061 0.11 +1.6 61,492 + rfbG Synpcc7942_0061 0.11 -0.7 61,498 + rfbG Synpcc7942_0061 0.12 -2.8 61,541 - rfbG Synpcc7942_0061 0.16 +0.8 61,543 + rfbG Synpcc7942_0061 0.16 +0.4 61,548 + rfbG Synpcc7942_0061 0.16 -3.4 61,578 + rfbG Synpcc7942_0061 0.19 +0.6 61,578 + rfbG Synpcc7942_0061 0.19 +0.8 61,586 - rfbG Synpcc7942_0061 0.20 -0.6 61,599 - rfbG Synpcc7942_0061 0.21 +0.9 61,601 + rfbG Synpcc7942_0061 0.21 -4.1 61,609 - rfbG Synpcc7942_0061 0.22 -2.8 61,634 - rfbG Synpcc7942_0061 0.24 +0.6 61,635 + rfbG Synpcc7942_0061 0.24 -2.8 61,658 - rfbG Synpcc7942_0061 0.26 -2.4 61,667 + rfbG Synpcc7942_0061 0.27 +0.7 61,693 + rfbG Synpcc7942_0061 0.30 -0.7 61,703 - rfbG Synpcc7942_0061 0.30 -0.2 61,723 + rfbG Synpcc7942_0061 0.32 +0.4 61,733 + rfbG Synpcc7942_0061 0.33 -2.4 61,733 - rfbG Synpcc7942_0061 0.33 -3.1 61,764 + rfbG Synpcc7942_0061 0.36 +1.3 61,764 + rfbG Synpcc7942_0061 0.36 -2.6 61,812 - rfbG Synpcc7942_0061 0.40 +1.2 61,812 - rfbG Synpcc7942_0061 0.40 +0.9 61,814 + rfbG Synpcc7942_0061 0.40 +1.5 61,845 - rfbG Synpcc7942_0061 0.43 +1.5 61,847 + rfbG Synpcc7942_0061 0.43 -0.4 61,850 + rfbG Synpcc7942_0061 0.44 -0.1 61,855 - rfbG Synpcc7942_0061 0.44 -2.2 61,855 - rfbG Synpcc7942_0061 0.44 -0.1 61,954 + rfbG Synpcc7942_0061 0.53 -1.4 61,960 + rfbG Synpcc7942_0061 0.54 +0.2 61,960 + rfbG Synpcc7942_0061 0.54 -2.9 61,970 + rfbG Synpcc7942_0061 0.55 +0.3 61,970 + rfbG Synpcc7942_0061 0.55 +0.6 61,978 - rfbG Synpcc7942_0061 0.55 -2.0 61,982 + rfbG Synpcc7942_0061 0.56 +0.4 61,984 + rfbG Synpcc7942_0061 0.56 +1.3 61,984 + rfbG Synpcc7942_0061 0.56 +0.2 61,990 + rfbG Synpcc7942_0061 0.56 -2.4 61,990 - rfbG Synpcc7942_0061 0.56 +0.5 61,990 - rfbG Synpcc7942_0061 0.56 +1.1 61,992 - rfbG Synpcc7942_0061 0.57 -1.2 62,025 + rfbG Synpcc7942_0061 0.60 +0.5 62,031 - rfbG Synpcc7942_0061 0.60 +1.2 62,031 - rfbG Synpcc7942_0061 0.60 -0.4 62,031 - rfbG Synpcc7942_0061 0.60 +0.9 62,060 - rfbG Synpcc7942_0061 0.63 -0.8 62,060 - rfbG Synpcc7942_0061 0.63 -0.4 62,086 - rfbG Synpcc7942_0061 0.65 -2.7 62,086 - rfbG Synpcc7942_0061 0.65 -0.4 62,086 - rfbG Synpcc7942_0061 0.65 -0.5 62,086 - rfbG Synpcc7942_0061 0.65 -0.2 62,096 + rfbG Synpcc7942_0061 0.66 +0.7 62,115 + rfbG Synpcc7942_0061 0.68 -1.6 62,130 + rfbG Synpcc7942_0061 0.69 -1.4 62,147 + rfbG Synpcc7942_0061 0.71 -1.0 62,170 - rfbG Synpcc7942_0061 0.73 +1.3 62,176 + rfbG Synpcc7942_0061 0.73 -1.1 62,184 - rfbG Synpcc7942_0061 0.74 -1.2 62,184 - rfbG Synpcc7942_0061 0.74 +0.3 62,195 + rfbG Synpcc7942_0061 0.75 +0.0 62,203 - rfbG Synpcc7942_0061 0.76 -2.6 62,205 - rfbG Synpcc7942_0061 0.76 +0.0 62,205 - rfbG Synpcc7942_0061 0.76 +0.8 62,231 - rfbG Synpcc7942_0061 0.78 +0.0 62,236 + rfbG Synpcc7942_0061 0.79 +0.7 62,249 + rfbG Synpcc7942_0061 0.80 +0.3 62,284 + rfbG Synpcc7942_0061 0.83 +0.0 62,314 + rfbG Synpcc7942_0061 0.86 -0.9 62,314 + rfbG Synpcc7942_0061 0.86 -0.4 62,314 + rfbG Synpcc7942_0061 0.86 +0.5 62,314 + -2.4 62,314 + rfbG Synpcc7942_0061 0.86 -0.4 62,314 + rfbG Synpcc7942_0061 0.86 -2.6 62,322 - rfbG Synpcc7942_0061 0.86 -0.1 62,329 - rfbG Synpcc7942_0061 0.87 -0.1 62,338 + rfbG Synpcc7942_0061 0.88 +0.2 62,355 + rfbG Synpcc7942_0061 0.89 -0.1 62,378 - +0.4 62,378 - -0.5 62,434 + +0.5 62,434 + +1.2 62,434 + -3.6 62,440 - +0.1 62,440 - -2.1 62,440 - +0.7 62,442 - -2.5 62,442 - +1.7 62,442 - -0.3 62,450 - -0.9 62,459 + -4.3 62,480 + -0.5 62,488 - +1.8 62,488 - -0.2 62,503 + +0.3 62,511 - -0.1 62,513 + +0.5 62,513 + -0.1 62,513 + +1.0 62,513 + +0.9 62,513 + +0.1 62,522 - +0.3 62,531 + +0.8 62,547 - rfbF Synpcc7942_0062 0.10 +2.3 62,578 - rfbF Synpcc7942_0062 0.14 +1.6 62,588 - rfbF Synpcc7942_0062 0.15 -0.4 62,740 - rfbF Synpcc7942_0062 0.35 +1.7 62,741 + rfbF Synpcc7942_0062 0.35 -3.7 62,753 + rfbF Synpcc7942_0062 0.37 +0.5 62,755 - rfbF Synpcc7942_0062 0.37 +2.0 62,756 - rfbF Synpcc7942_0062 0.37 +1.0 62,939 - rfbF Synpcc7942_0062 0.61 +0.3 62,964 - rfbF Synpcc7942_0062 0.64 -0.3 63,181 - -2.0 63,286 + -0.3
Or see this region's nucleotide sequence