Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4362 and Pf6N2E2_4363 are separated by 167 nucleotides Pf6N2E2_4363 and Pf6N2E2_4364 are separated by 10 nucleotides Pf6N2E2_4364 and Pf6N2E2_4365 are separated by 271 nucleotides
Pf6N2E2_4362 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 5,093,118 to 5,093,381
_4362
Pf6N2E2_4363 - Guanylate kinase (EC 2.7.4.8), at 5,093,549 to 5,094,169
_4363
Pf6N2E2_4364 - Protein YicC, at 5,094,180 to 5,095,043
_4364
Pf6N2E2_4365 - Ribonuclease PH (EC 2.7.7.56), at 5,095,315 to 5,096,037
_4365
Position (kb)
5094
5095
5096 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5093.201 kb on - strand, within Pf6N2E2_4362 at 5093.236 kb on - strand, within Pf6N2E2_4362 at 5093.239 kb on - strand, within Pf6N2E2_4362 at 5093.244 kb on - strand, within Pf6N2E2_4362 at 5093.244 kb on - strand, within Pf6N2E2_4362 at 5093.244 kb on - strand, within Pf6N2E2_4362 at 5093.244 kb on - strand, within Pf6N2E2_4362 at 5093.244 kb on - strand, within Pf6N2E2_4362 at 5093.385 kb on - strand at 5093.385 kb on - strand at 5093.508 kb on + strand at 5093.508 kb on + strand at 5093.516 kb on + strand at 5093.516 kb on + strand at 5093.516 kb on + strand at 5093.516 kb on + strand at 5093.516 kb on + strand at 5093.517 kb on - strand at 5093.517 kb on - strand at 5093.517 kb on - strand at 5093.517 kb on - strand at 5093.517 kb on - strand at 5093.570 kb on + strand at 5093.571 kb on - strand at 5093.571 kb on - strand at 5093.571 kb on - strand at 5093.571 kb on - strand at 5094.182 kb on - strand at 5094.182 kb on - strand at 5094.282 kb on - strand, within Pf6N2E2_4364 at 5094.282 kb on - strand, within Pf6N2E2_4364 at 5094.282 kb on - strand, within Pf6N2E2_4364 at 5094.282 kb on - strand, within Pf6N2E2_4364 at 5095.134 kb on + strand at 5095.135 kb on - strand at 5095.254 kb on + strand at 5095.272 kb on + strand at 5095.272 kb on + strand at 5095.272 kb on + strand at 5095.273 kb on - strand at 5095.273 kb on - strand at 5095.273 kb on - strand at 5095.273 kb on - strand at 5095.274 kb on + strand at 5095.274 kb on + strand at 5095.274 kb on + strand at 5095.274 kb on + strand at 5095.307 kb on + strand at 5095.307 kb on + strand at 5095.366 kb on - strand at 5095.375 kb on + strand at 5095.376 kb on - strand at 5095.376 kb on - strand at 5095.401 kb on - strand, within Pf6N2E2_4365 at 5095.401 kb on - strand, within Pf6N2E2_4365 at 5095.420 kb on - strand, within Pf6N2E2_4365 at 5095.420 kb on - strand, within Pf6N2E2_4365 at 5095.654 kb on + strand, within Pf6N2E2_4365 at 5095.654 kb on + strand, within Pf6N2E2_4365 at 5095.654 kb on + strand, within Pf6N2E2_4365 at 5095.655 kb on - strand, within Pf6N2E2_4365 at 5095.655 kb on - strand, within Pf6N2E2_4365 at 5095.655 kb on - strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.819 kb on + strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.820 kb on - strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.849 kb on + strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.850 kb on - strand, within Pf6N2E2_4365 at 5095.886 kb on + strand, within Pf6N2E2_4365 at 5095.886 kb on + strand, within Pf6N2E2_4365 at 5095.887 kb on - strand, within Pf6N2E2_4365 at 5095.887 kb on - strand, within Pf6N2E2_4365 at 5095.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 5,093,201 - Pf6N2E2_4362 0.31 -0.3 5,093,236 - Pf6N2E2_4362 0.45 +1.0 5,093,239 - Pf6N2E2_4362 0.46 -2.4 5,093,244 - Pf6N2E2_4362 0.48 -1.6 5,093,244 - Pf6N2E2_4362 0.48 -0.8 5,093,244 - Pf6N2E2_4362 0.48 -2.8 5,093,244 - Pf6N2E2_4362 0.48 -2.0 5,093,244 - Pf6N2E2_4362 0.48 +0.4 5,093,385 - -3.0 5,093,385 - -1.8 5,093,508 + -1.0 5,093,508 + +0.3 5,093,516 + +0.7 5,093,516 + +0.0 5,093,516 + +0.7 5,093,516 + +0.5 5,093,516 + +0.4 5,093,517 - +1.0 5,093,517 - +0.4 5,093,517 - -1.5 5,093,517 - +1.5 5,093,517 - -0.2 5,093,570 + -2.1 5,093,571 - +0.1 5,093,571 - -1.3 5,093,571 - +0.4 5,093,571 - +0.7 5,094,182 - +0.3 5,094,182 - -0.0 5,094,282 - Pf6N2E2_4364 0.12 -1.5 5,094,282 - Pf6N2E2_4364 0.12 -0.3 5,094,282 - Pf6N2E2_4364 0.12 -3.4 5,094,282 - Pf6N2E2_4364 0.12 -0.4 5,095,134 + +0.2 5,095,135 - +0.2 5,095,254 + -0.8 5,095,272 + +1.3 5,095,272 + +0.4 5,095,272 + +1.3 5,095,273 - -0.5 5,095,273 - -3.0 5,095,273 - -0.6 5,095,273 - -1.0 5,095,274 + -0.6 5,095,274 + +0.4 5,095,274 + +0.4 5,095,274 + -0.1 5,095,307 + -0.7 5,095,307 + +1.2 5,095,366 - -0.7 5,095,375 + -1.4 5,095,376 - +0.6 5,095,376 - -0.2 5,095,401 - Pf6N2E2_4365 0.12 +2.1 5,095,401 - Pf6N2E2_4365 0.12 +3.0 5,095,420 - Pf6N2E2_4365 0.15 +0.6 5,095,420 - Pf6N2E2_4365 0.15 -0.1 5,095,654 + Pf6N2E2_4365 0.47 +0.8 5,095,654 + Pf6N2E2_4365 0.47 +0.5 5,095,654 + Pf6N2E2_4365 0.47 +1.4 5,095,655 - Pf6N2E2_4365 0.47 +1.2 5,095,655 - Pf6N2E2_4365 0.47 +1.3 5,095,655 - Pf6N2E2_4365 0.47 +1.1 5,095,819 + Pf6N2E2_4365 0.70 +0.7 5,095,819 + Pf6N2E2_4365 0.70 +1.3 5,095,819 + Pf6N2E2_4365 0.70 +1.8 5,095,820 - Pf6N2E2_4365 0.70 +0.6 5,095,820 - Pf6N2E2_4365 0.70 +1.3 5,095,820 - Pf6N2E2_4365 0.70 +0.4 5,095,820 - Pf6N2E2_4365 0.70 -1.0 5,095,820 - Pf6N2E2_4365 0.70 +1.1 5,095,820 - Pf6N2E2_4365 0.70 +0.8 5,095,849 + Pf6N2E2_4365 0.74 +2.0 5,095,849 + Pf6N2E2_4365 0.74 +0.5 5,095,849 + Pf6N2E2_4365 0.74 +1.1 5,095,850 - Pf6N2E2_4365 0.74 +0.6 5,095,850 - Pf6N2E2_4365 0.74 -1.0 5,095,850 - Pf6N2E2_4365 0.74 +0.2 5,095,886 + Pf6N2E2_4365 0.79 +0.1 5,095,886 + Pf6N2E2_4365 0.79 +0.6 5,095,887 - Pf6N2E2_4365 0.79 +0.7 5,095,887 - Pf6N2E2_4365 0.79 +0.5 5,095,969 - +0.8
Or see this region's nucleotide sequence