Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3170 and Pf6N2E2_3171 are separated by 194 nucleotides Pf6N2E2_3171 and Pf6N2E2_3172 are separated by 63 nucleotides Pf6N2E2_3172 and Pf6N2E2_3173 overlap by 13 nucleotides
Pf6N2E2_3170 - 2-isopropylmalate synthase (EC 2.3.3.13), at 3,665,153 to 3,666,832
_3170
Pf6N2E2_3171 - hypothetical protein, at 3,667,027 to 3,667,440
_3171
Pf6N2E2_3172 - Aliphatic amidase AmiE (EC 3.5.1.4), at 3,667,504 to 3,668,298
_3172
Pf6N2E2_3173 - Aspartate aminotransferase (EC 2.6.1.1), at 3,668,286 to 3,669,434
_3173
Position (kb)
3667
3668 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3666.029 kb on + strand, within Pf6N2E2_3170 at 3666.131 kb on + strand, within Pf6N2E2_3170 at 3666.132 kb on - strand, within Pf6N2E2_3170 at 3666.132 kb on - strand, within Pf6N2E2_3170 at 3666.132 kb on - strand, within Pf6N2E2_3170 at 3666.233 kb on + strand, within Pf6N2E2_3170 at 3666.233 kb on + strand, within Pf6N2E2_3170 at 3666.233 kb on + strand, within Pf6N2E2_3170 at 3666.234 kb on - strand, within Pf6N2E2_3170 at 3666.234 kb on - strand, within Pf6N2E2_3170 at 3666.234 kb on - strand, within Pf6N2E2_3170 at 3666.269 kb on + strand, within Pf6N2E2_3170 at 3666.269 kb on + strand, within Pf6N2E2_3170 at 3666.269 kb on + strand, within Pf6N2E2_3170 at 3666.270 kb on - strand, within Pf6N2E2_3170 at 3666.270 kb on - strand, within Pf6N2E2_3170 at 3666.339 kb on - strand, within Pf6N2E2_3170 at 3666.387 kb on + strand, within Pf6N2E2_3170 at 3666.387 kb on + strand, within Pf6N2E2_3170 at 3666.387 kb on + strand at 3666.387 kb on + strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.458 kb on + strand, within Pf6N2E2_3170 at 3666.459 kb on - strand, within Pf6N2E2_3170 at 3666.529 kb on - strand, within Pf6N2E2_3170 at 3666.647 kb on + strand, within Pf6N2E2_3170 at 3666.647 kb on + strand, within Pf6N2E2_3170 at 3666.647 kb on + strand, within Pf6N2E2_3170 at 3666.648 kb on - strand, within Pf6N2E2_3170 at 3666.648 kb on - strand, within Pf6N2E2_3170 at 3666.695 kb on + strand at 3666.835 kb on + strand at 3666.835 kb on + strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.912 kb on + strand at 3666.912 kb on + strand at 3666.912 kb on + strand at 3666.913 kb on - strand at 3667.389 kb on + strand, within Pf6N2E2_3171 at 3667.414 kb on + strand at 3667.414 kb on + strand at 3667.431 kb on + strand at 3667.432 kb on - strand at 3667.440 kb on + strand at 3667.559 kb on + strand at 3667.667 kb on + strand, within Pf6N2E2_3172 at 3667.667 kb on + strand, within Pf6N2E2_3172 at 3667.668 kb on - strand, within Pf6N2E2_3172 at 3667.715 kb on + strand, within Pf6N2E2_3172 at 3667.716 kb on - strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.795 kb on + strand, within Pf6N2E2_3172 at 3667.795 kb on + strand, within Pf6N2E2_3172 at 3667.796 kb on - strand, within Pf6N2E2_3172 at 3667.796 kb on - strand, within Pf6N2E2_3172 at 3667.851 kb on - strand, within Pf6N2E2_3172 at 3667.916 kb on + strand, within Pf6N2E2_3172 at 3667.917 kb on - strand, within Pf6N2E2_3172 at 3667.961 kb on + strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3668.298 kb on + strand at 3668.298 kb on + strand at 3668.299 kb on - strand at 3668.299 kb on - strand at 3668.299 kb on - strand at 3668.383 kb on + strand at 3668.384 kb on - strand at 3668.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 3,666,029 + Pf6N2E2_3170 0.52 -3.9 3,666,131 + Pf6N2E2_3170 0.58 -4.0 3,666,132 - Pf6N2E2_3170 0.58 -3.2 3,666,132 - Pf6N2E2_3170 0.58 -2.2 3,666,132 - Pf6N2E2_3170 0.58 -4.1 3,666,233 + Pf6N2E2_3170 0.64 -2.9 3,666,233 + Pf6N2E2_3170 0.64 -2.5 3,666,233 + Pf6N2E2_3170 0.64 -1.8 3,666,234 - Pf6N2E2_3170 0.64 -3.0 3,666,234 - Pf6N2E2_3170 0.64 -1.5 3,666,234 - Pf6N2E2_3170 0.64 -3.2 3,666,269 + Pf6N2E2_3170 0.66 -1.6 3,666,269 + Pf6N2E2_3170 0.66 -1.9 3,666,269 + Pf6N2E2_3170 0.66 -2.4 3,666,270 - Pf6N2E2_3170 0.66 -2.7 3,666,270 - Pf6N2E2_3170 0.66 -3.4 3,666,339 - Pf6N2E2_3170 0.71 -3.5 3,666,387 + Pf6N2E2_3170 0.73 -2.6 3,666,387 + Pf6N2E2_3170 0.73 -2.9 3,666,387 + +1.2 3,666,387 + Pf6N2E2_3170 0.73 -3.9 3,666,388 - Pf6N2E2_3170 0.74 -3.1 3,666,388 - Pf6N2E2_3170 0.74 -3.9 3,666,388 - Pf6N2E2_3170 0.74 -2.2 3,666,388 - Pf6N2E2_3170 0.74 -4.0 3,666,458 + Pf6N2E2_3170 0.78 +0.5 3,666,459 - Pf6N2E2_3170 0.78 +0.4 3,666,529 - Pf6N2E2_3170 0.82 +1.2 3,666,647 + Pf6N2E2_3170 0.89 +0.3 3,666,647 + Pf6N2E2_3170 0.89 +0.8 3,666,647 + Pf6N2E2_3170 0.89 +0.2 3,666,648 - Pf6N2E2_3170 0.89 +0.8 3,666,648 - Pf6N2E2_3170 0.89 +0.1 3,666,695 + -0.6 3,666,835 + -1.0 3,666,835 + +0.7 3,666,836 - +1.0 3,666,836 - +0.5 3,666,836 - +0.7 3,666,836 - +0.4 3,666,836 - -0.2 3,666,912 + -1.5 3,666,912 + +0.7 3,666,912 + -1.0 3,666,913 - -0.2 3,667,389 + Pf6N2E2_3171 0.87 -1.0 3,667,414 + +1.1 3,667,414 + +1.2 3,667,431 + +0.7 3,667,432 - -0.1 3,667,440 + +0.8 3,667,559 + +0.7 3,667,667 + Pf6N2E2_3172 0.21 +0.5 3,667,667 + Pf6N2E2_3172 0.21 -0.7 3,667,668 - Pf6N2E2_3172 0.21 +1.2 3,667,715 + Pf6N2E2_3172 0.27 -0.4 3,667,716 - Pf6N2E2_3172 0.27 -0.4 3,667,793 + Pf6N2E2_3172 0.36 +0.6 3,667,793 + Pf6N2E2_3172 0.36 +0.1 3,667,793 + Pf6N2E2_3172 0.36 +0.6 3,667,793 + Pf6N2E2_3172 0.36 +0.3 3,667,793 + Pf6N2E2_3172 0.36 -0.8 3,667,794 - Pf6N2E2_3172 0.36 +0.7 3,667,794 - Pf6N2E2_3172 0.36 +1.0 3,667,794 - Pf6N2E2_3172 0.36 +0.8 3,667,794 - Pf6N2E2_3172 0.36 +0.9 3,667,794 - Pf6N2E2_3172 0.36 -0.4 3,667,794 - Pf6N2E2_3172 0.36 +0.1 3,667,795 + Pf6N2E2_3172 0.37 -0.3 3,667,795 + Pf6N2E2_3172 0.37 +0.0 3,667,796 - Pf6N2E2_3172 0.37 -0.8 3,667,796 - Pf6N2E2_3172 0.37 +1.0 3,667,851 - Pf6N2E2_3172 0.44 +0.0 3,667,916 + Pf6N2E2_3172 0.52 +1.1 3,667,917 - Pf6N2E2_3172 0.52 +0.4 3,667,961 + Pf6N2E2_3172 0.57 +0.2 3,667,962 - Pf6N2E2_3172 0.58 -0.2 3,667,962 - Pf6N2E2_3172 0.58 +0.9 3,667,962 - Pf6N2E2_3172 0.58 -0.9 3,668,298 + +0.2 3,668,298 + +0.7 3,668,299 - +0.3 3,668,299 - -0.1 3,668,299 - +0.0 3,668,383 + +0.5 3,668,384 - +0.3 3,668,384 - +0.1
Or see this region's nucleotide sequence