Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2177 and Pf6N2E2_2178 overlap by 17 nucleotides Pf6N2E2_2178 and Pf6N2E2_2179 are separated by 76 nucleotides
Pf6N2E2_2177 - Glutamine synthetase (EC 6.3.1.2), putative, at 2,581,513 to 2,582,868
_2177
Pf6N2E2_2178 - FIG00960144: hypothetical protein, at 2,582,852 to 2,583,604
_2178
Pf6N2E2_2179 - Putrescine importer, at 2,583,681 to 2,585,030
_2179
Position (kb)
2582
2583
2584 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2582.092 kb on + strand, within Pf6N2E2_2177 at 2582.151 kb on - strand, within Pf6N2E2_2177 at 2582.353 kb on + strand, within Pf6N2E2_2177 at 2582.398 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.782 kb on + strand at 2582.782 kb on + strand at 2582.782 kb on + strand at 2582.783 kb on - strand at 2582.851 kb on + strand at 2582.851 kb on + strand at 2582.851 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.864 kb on - strand at 2582.888 kb on + strand at 2582.888 kb on + strand at 2582.889 kb on - strand at 2582.889 kb on - strand at 2583.101 kb on + strand, within Pf6N2E2_2178 at 2583.101 kb on + strand, within Pf6N2E2_2178 at 2583.151 kb on + strand, within Pf6N2E2_2178 at 2583.151 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.174 kb on - strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.392 kb on + strand, within Pf6N2E2_2178 at 2583.392 kb on + strand, within Pf6N2E2_2178 at 2583.455 kb on + strand, within Pf6N2E2_2178 at 2583.455 kb on + strand, within Pf6N2E2_2178 at 2583.584 kb on + strand at 2583.585 kb on - strand at 2583.602 kb on + strand at 2583.602 kb on + strand at 2583.602 kb on + strand at 2583.748 kb on + strand at 2583.748 kb on + strand at 2583.749 kb on - strand at 2583.792 kb on + strand at 2583.798 kb on + strand at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.879 kb on + strand, within Pf6N2E2_2179 at 2583.922 kb on - strand, within Pf6N2E2_2179 at 2583.922 kb on - strand, within Pf6N2E2_2179 at 2584.014 kb on + strand, within Pf6N2E2_2179 at 2584.014 kb on + strand, within Pf6N2E2_2179 at 2584.014 kb on + strand, within Pf6N2E2_2179 at 2584.020 kb on + strand, within Pf6N2E2_2179 at 2584.020 kb on + strand, within Pf6N2E2_2179 at 2584.020 kb on + strand, within Pf6N2E2_2179 at 2584.021 kb on - strand, within Pf6N2E2_2179 at 2584.021 kb on - strand, within Pf6N2E2_2179 at 2584.192 kb on - strand, within Pf6N2E2_2179 at 2584.192 kb on - strand, within Pf6N2E2_2179 at 2584.251 kb on + strand, within Pf6N2E2_2179
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,582,092 + Pf6N2E2_2177 0.43 +1.2 2,582,151 - Pf6N2E2_2177 0.47 +1.4 2,582,353 + Pf6N2E2_2177 0.62 +0.2 2,582,398 + Pf6N2E2_2177 0.65 -0.8 2,582,434 + Pf6N2E2_2177 0.68 -0.5 2,582,434 + Pf6N2E2_2177 0.68 -0.3 2,582,434 + Pf6N2E2_2177 0.68 -0.8 2,582,435 - Pf6N2E2_2177 0.68 +1.4 2,582,435 - Pf6N2E2_2177 0.68 +1.4 2,582,435 - Pf6N2E2_2177 0.68 +0.3 2,582,435 - Pf6N2E2_2177 0.68 +0.4 2,582,782 + -1.9 2,582,782 + -0.4 2,582,782 + +0.3 2,582,783 - +1.0 2,582,851 + +0.4 2,582,851 + +0.1 2,582,851 + -0.5 2,582,863 + +1.2 2,582,863 + -0.1 2,582,863 + +0.3 2,582,863 + +1.6 2,582,864 - +0.6 2,582,888 + -1.4 2,582,888 + -0.4 2,582,889 - +1.5 2,582,889 - +2.4 2,583,101 + Pf6N2E2_2178 0.33 -0.9 2,583,101 + Pf6N2E2_2178 0.33 -0.4 2,583,151 + Pf6N2E2_2178 0.40 -2.1 2,583,151 + Pf6N2E2_2178 0.40 -0.9 2,583,173 + Pf6N2E2_2178 0.43 -2.2 2,583,173 + Pf6N2E2_2178 0.43 -0.9 2,583,173 + Pf6N2E2_2178 0.43 -1.8 2,583,173 + Pf6N2E2_2178 0.43 -2.6 2,583,174 - Pf6N2E2_2178 0.43 +1.5 2,583,338 + Pf6N2E2_2178 0.65 -1.6 2,583,338 + Pf6N2E2_2178 0.65 -0.8 2,583,338 + Pf6N2E2_2178 0.65 -0.8 2,583,338 + Pf6N2E2_2178 0.65 -1.4 2,583,392 + Pf6N2E2_2178 0.72 -1.1 2,583,392 + Pf6N2E2_2178 0.72 -1.4 2,583,455 + Pf6N2E2_2178 0.80 -1.8 2,583,455 + Pf6N2E2_2178 0.80 -3.0 2,583,584 + -2.4 2,583,585 - +0.9 2,583,602 + -2.2 2,583,602 + -1.9 2,583,602 + -0.1 2,583,748 + +1.6 2,583,748 + +1.1 2,583,749 - -0.4 2,583,792 + +0.1 2,583,798 + +1.3 2,583,838 - Pf6N2E2_2179 0.12 +0.3 2,583,838 - Pf6N2E2_2179 0.12 +1.9 2,583,838 - Pf6N2E2_2179 0.12 +0.4 2,583,838 - Pf6N2E2_2179 0.12 -1.1 2,583,879 + Pf6N2E2_2179 0.15 -0.1 2,583,922 - Pf6N2E2_2179 0.18 -0.6 2,583,922 - Pf6N2E2_2179 0.18 +0.1 2,584,014 + Pf6N2E2_2179 0.25 +1.2 2,584,014 + Pf6N2E2_2179 0.25 +0.6 2,584,014 + Pf6N2E2_2179 0.25 +0.7 2,584,020 + Pf6N2E2_2179 0.25 -0.5 2,584,020 + Pf6N2E2_2179 0.25 +2.0 2,584,020 + Pf6N2E2_2179 0.25 -0.1 2,584,021 - Pf6N2E2_2179 0.25 +0.2 2,584,021 - Pf6N2E2_2179 0.25 -0.5 2,584,192 - Pf6N2E2_2179 0.38 +1.5 2,584,192 - Pf6N2E2_2179 0.38 +0.2 2,584,251 + Pf6N2E2_2179 0.42 +0.8
Or see this region's nucleotide sequence