Experiment: CDM1 without Isoleucine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20925 and BBR_RS15835 are separated by 201 nucleotides BBR_RS15835 and BBR_RS15840 are separated by 183 nucleotides BBR_RS15840 and BBR_RS20930 are separated by 330 nucleotides BBR_RS20930 and BBR_RS15845 are separated by 56 nucleotides BBR_RS15845 and BBR_RS20935 are separated by 10 nucleotides
BBR_RS20925: BBR_RS20925 - amino acid permease, at 1,318,242 to 1,318,397
_RS20925
BBR_RS15835: BBR_RS15835 - MFS transporter, at 1,318,599 to 1,319,207
_RS15835
BBR_RS15840: BBR_RS15840 - VanZ family protein, at 1,319,391 to 1,320,404
_RS15840
BBR_RS20930: BBR_RS20930 - hypothetical protein, at 1,320,735 to 1,321,001
_RS20930
BBR_RS15845: BBR_RS15845 - hypothetical protein, at 1,321,058 to 1,321,327
_RS15845
BBR_RS20935: BBR_RS20935 - hypothetical protein, at 1,321,338 to 1,321,659
_RS20935
Position (kb)
1319
1320
1321 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1318.534 kb on - strand at 1318.542 kb on - strand at 1318.542 kb on - strand at 1318.552 kb on - strand at 1318.554 kb on - strand at 1318.556 kb on + strand at 1318.566 kb on + strand at 1318.659 kb on + strand at 1318.667 kb on - strand, within BBR_RS15835 at 1318.669 kb on - strand, within BBR_RS15835 at 1318.743 kb on - strand, within BBR_RS15835 at 1318.745 kb on + strand, within BBR_RS15835 at 1318.753 kb on - strand, within BBR_RS15835 at 1318.770 kb on + strand, within BBR_RS15835 at 1318.861 kb on + strand, within BBR_RS15835 at 1318.889 kb on - strand, within BBR_RS15835 at 1318.994 kb on + strand, within BBR_RS15835 at 1319.002 kb on - strand, within BBR_RS15835 at 1319.013 kb on + strand, within BBR_RS15835 at 1319.070 kb on + strand, within BBR_RS15835 at 1319.085 kb on - strand, within BBR_RS15835 at 1319.091 kb on - strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.117 kb on + strand, within BBR_RS15835 at 1319.120 kb on - strand, within BBR_RS15835 at 1319.120 kb on - strand, within BBR_RS15835 at 1319.129 kb on + strand, within BBR_RS15835 at 1319.150 kb on + strand at 1319.224 kb on - strand at 1319.259 kb on - strand at 1319.269 kb on + strand at 1319.277 kb on + strand at 1319.289 kb on - strand at 1319.523 kb on - strand, within BBR_RS15840 at 1319.523 kb on - strand, within BBR_RS15840 at 1319.532 kb on + strand, within BBR_RS15840 at 1319.651 kb on + strand, within BBR_RS15840 at 1319.660 kb on - strand, within BBR_RS15840 at 1319.695 kb on - strand, within BBR_RS15840 at 1319.717 kb on - strand, within BBR_RS15840 at 1319.722 kb on - strand, within BBR_RS15840 at 1319.824 kb on - strand, within BBR_RS15840 at 1319.869 kb on + strand, within BBR_RS15840 at 1319.879 kb on + strand, within BBR_RS15840 at 1319.892 kb on - strand, within BBR_RS15840 at 1320.075 kb on + strand, within BBR_RS15840 at 1320.114 kb on - strand, within BBR_RS15840 at 1320.129 kb on + strand, within BBR_RS15840 at 1320.132 kb on - strand, within BBR_RS15840 at 1320.146 kb on + strand, within BBR_RS15840 at 1320.152 kb on - strand, within BBR_RS15840 at 1320.214 kb on - strand, within BBR_RS15840 at 1320.226 kb on - strand, within BBR_RS15840 at 1320.226 kb on - strand, within BBR_RS15840 at 1320.228 kb on - strand, within BBR_RS15840 at 1320.240 kb on - strand, within BBR_RS15840 at 1320.248 kb on + strand, within BBR_RS15840 at 1320.251 kb on - strand, within BBR_RS15840 at 1320.251 kb on - strand, within BBR_RS15840 at 1320.335 kb on - strand at 1320.357 kb on - strand at 1320.427 kb on - strand at 1320.474 kb on + strand at 1320.474 kb on + strand at 1320.474 kb on + strand at 1320.525 kb on + strand at 1320.564 kb on - strand at 1320.615 kb on + strand at 1320.669 kb on - strand at 1320.669 kb on - strand at 1320.733 kb on + strand at 1320.856 kb on + strand, within BBR_RS20930 at 1320.864 kb on - strand, within BBR_RS20930 at 1320.951 kb on - strand, within BBR_RS20930 at 1321.013 kb on - strand at 1321.040 kb on - strand at 1321.130 kb on - strand, within BBR_RS15845 at 1321.285 kb on + strand, within BBR_RS15845 at 1321.318 kb on + strand at 1321.401 kb on + strand, within BBR_RS20935
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Isoleucine remove 1,318,534 - -1.1 1,318,542 - +0.4 1,318,542 - -1.2 1,318,552 - -1.9 1,318,554 - -1.5 1,318,556 + +0.5 1,318,566 + +1.1 1,318,659 + -0.1 1,318,667 - BBR_RS15835 0.11 +0.4 1,318,669 - BBR_RS15835 0.11 +0.7 1,318,743 - BBR_RS15835 0.24 -1.4 1,318,745 + BBR_RS15835 0.24 -0.7 1,318,753 - BBR_RS15835 0.25 -1.2 1,318,770 + BBR_RS15835 0.28 -1.5 1,318,861 + BBR_RS15835 0.43 -3.5 1,318,889 - BBR_RS15835 0.48 +0.5 1,318,994 + BBR_RS15835 0.65 +0.3 1,319,002 - BBR_RS15835 0.66 -0.4 1,319,013 + BBR_RS15835 0.68 -0.9 1,319,070 + BBR_RS15835 0.77 +1.7 1,319,085 - BBR_RS15835 0.80 +0.3 1,319,091 - BBR_RS15835 0.81 -0.5 1,319,112 + BBR_RS15835 0.84 -0.1 1,319,112 + BBR_RS15835 0.84 +1.3 1,319,112 + BBR_RS15835 0.84 -0.0 1,319,112 + BBR_RS15835 0.84 +0.6 1,319,112 + BBR_RS15835 0.84 +1.0 1,319,112 + BBR_RS15835 0.84 +0.6 1,319,117 + BBR_RS15835 0.85 -0.6 1,319,120 - BBR_RS15835 0.86 +1.0 1,319,120 - BBR_RS15835 0.86 -0.6 1,319,129 + BBR_RS15835 0.87 +0.7 1,319,150 + +0.2 1,319,224 - +0.1 1,319,259 - +0.3 1,319,269 + +0.7 1,319,277 + -0.5 1,319,289 - +0.9 1,319,523 - BBR_RS15840 0.13 -0.4 1,319,523 - BBR_RS15840 0.13 +0.4 1,319,532 + BBR_RS15840 0.14 -1.3 1,319,651 + BBR_RS15840 0.26 -1.0 1,319,660 - BBR_RS15840 0.27 +0.5 1,319,695 - BBR_RS15840 0.30 +0.1 1,319,717 - BBR_RS15840 0.32 -1.3 1,319,722 - BBR_RS15840 0.33 -1.5 1,319,824 - BBR_RS15840 0.43 +1.3 1,319,869 + BBR_RS15840 0.47 -0.9 1,319,879 + BBR_RS15840 0.48 -1.3 1,319,892 - BBR_RS15840 0.49 +1.0 1,320,075 + BBR_RS15840 0.67 -1.9 1,320,114 - BBR_RS15840 0.71 +0.9 1,320,129 + BBR_RS15840 0.73 +0.8 1,320,132 - BBR_RS15840 0.73 -0.7 1,320,146 + BBR_RS15840 0.74 -0.6 1,320,152 - BBR_RS15840 0.75 +0.0 1,320,214 - BBR_RS15840 0.81 -1.2 1,320,226 - BBR_RS15840 0.82 -0.2 1,320,226 - BBR_RS15840 0.82 -0.1 1,320,228 - BBR_RS15840 0.83 +1.1 1,320,240 - BBR_RS15840 0.84 -0.9 1,320,248 + BBR_RS15840 0.85 -2.8 1,320,251 - BBR_RS15840 0.85 -0.5 1,320,251 - BBR_RS15840 0.85 +0.5 1,320,335 - +0.0 1,320,357 - -0.2 1,320,427 - -0.4 1,320,474 + +0.5 1,320,474 + -2.3 1,320,474 + -1.3 1,320,525 + -0.1 1,320,564 - +0.7 1,320,615 + -0.1 1,320,669 - -0.2 1,320,669 - -1.2 1,320,733 + -2.9 1,320,856 + BBR_RS20930 0.45 -1.1 1,320,864 - BBR_RS20930 0.48 -1.4 1,320,951 - BBR_RS20930 0.81 -0.5 1,321,013 - -2.0 1,321,040 - -0.4 1,321,130 - BBR_RS15845 0.27 -1.2 1,321,285 + BBR_RS15845 0.84 -1.6 1,321,318 + -1.3 1,321,401 + BBR_RS20935 0.20 -2.3
Or see this region's nucleotide sequence