Experiment: CDM1 without Isoleucine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12200 and BBR_RS12205 are separated by 169 nucleotides BBR_RS12205 and BBR_RS12210 are separated by 245 nucleotides BBR_RS12210 and BBR_RS12215 are separated by 683 nucleotides
BBR_RS12200: BBR_RS12200 - hypothetical protein, at 484,980 to 485,678
_RS12200
BBR_RS12205: BBR_RS12205 - tRNA-Gly, at 485,848 to 485,921
_RS12205
BBR_RS12210: BBR_RS12210 - hypothetical protein, at 486,167 to 486,697
_RS12210
BBR_RS12215: BBR_RS12215 - VWA domain-containing protein, at 487,381 to 491,109
_RS12215
Position (kb) 486
487 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 485.185 kb on + strand, within BBR_RS12200 at 485.185 kb on + strand, within BBR_RS12200 at 485.185 kb on + strand, within BBR_RS12200 at 485.185 kb on + strand, within BBR_RS12200 at 485.187 kb on - strand, within BBR_RS12200 at 485.193 kb on - strand, within BBR_RS12200 at 485.200 kb on - strand, within BBR_RS12200 at 485.200 kb on - strand, within BBR_RS12200 at 485.206 kb on + strand, within BBR_RS12200 at 485.214 kb on - strand, within BBR_RS12200 at 485.219 kb on + strand, within BBR_RS12200 at 485.281 kb on + strand, within BBR_RS12200 at 485.281 kb on + strand, within BBR_RS12200 at 485.289 kb on - strand, within BBR_RS12200 at 485.289 kb on - strand, within BBR_RS12200 at 485.289 kb on - strand, within BBR_RS12200 at 485.291 kb on + strand, within BBR_RS12200 at 485.291 kb on + strand, within BBR_RS12200 at 485.296 kb on - strand, within BBR_RS12200 at 485.299 kb on - strand, within BBR_RS12200 at 485.304 kb on - strand, within BBR_RS12200 at 485.318 kb on + strand, within BBR_RS12200 at 485.318 kb on + strand, within BBR_RS12200 at 485.364 kb on + strand, within BBR_RS12200 at 485.364 kb on + strand, within BBR_RS12200 at 485.372 kb on - strand, within BBR_RS12200 at 485.372 kb on - strand, within BBR_RS12200 at 485.372 kb on - strand, within BBR_RS12200 at 485.377 kb on + strand, within BBR_RS12200 at 485.404 kb on - strand, within BBR_RS12200 at 485.404 kb on - strand, within BBR_RS12200 at 485.404 kb on - strand, within BBR_RS12200 at 485.456 kb on + strand, within BBR_RS12200 at 485.456 kb on + strand, within BBR_RS12200 at 485.464 kb on - strand, within BBR_RS12200 at 485.464 kb on - strand, within BBR_RS12200 at 485.475 kb on - strand, within BBR_RS12200 at 485.495 kb on - strand, within BBR_RS12200 at 485.512 kb on - strand, within BBR_RS12200 at 485.512 kb on - strand, within BBR_RS12200 at 485.512 kb on - strand, within BBR_RS12200 at 485.565 kb on - strand, within BBR_RS12200 at 485.565 kb on - strand, within BBR_RS12200 at 485.589 kb on - strand, within BBR_RS12200 at 485.618 kb on + strand at 485.623 kb on + strand at 485.623 kb on + strand at 485.623 kb on + strand at 485.623 kb on + strand at 485.630 kb on + strand at 485.636 kb on - strand at 485.636 kb on - strand at 485.655 kb on - strand at 485.673 kb on + strand at 485.682 kb on - strand at 485.693 kb on - strand at 485.712 kb on + strand at 485.712 kb on + strand at 485.722 kb on + strand at 485.722 kb on + strand at 485.725 kb on - strand at 485.730 kb on - strand at 485.730 kb on - strand at 485.739 kb on + strand at 485.747 kb on - strand at 485.750 kb on + strand at 485.752 kb on + strand at 485.758 kb on - strand at 485.758 kb on - strand at 485.758 kb on - strand at 485.758 kb on - strand at 485.760 kb on - strand at 485.766 kb on - strand at 485.799 kb on - strand at 485.961 kb on + strand at 485.970 kb on + strand at 485.970 kb on + strand at 485.989 kb on - strand at 486.005 kb on - strand at 486.085 kb on - strand at 486.119 kb on + strand at 486.134 kb on - strand at 486.152 kb on + strand at 486.193 kb on + strand at 486.275 kb on + strand, within BBR_RS12210 at 486.313 kb on + strand, within BBR_RS12210 at 486.318 kb on + strand, within BBR_RS12210 at 486.371 kb on + strand, within BBR_RS12210 at 486.381 kb on + strand, within BBR_RS12210 at 486.381 kb on + strand, within BBR_RS12210 at 486.381 kb on + strand, within BBR_RS12210 at 486.389 kb on - strand, within BBR_RS12210 at 486.389 kb on - strand, within BBR_RS12210 at 486.389 kb on - strand, within BBR_RS12210 at 486.447 kb on + strand, within BBR_RS12210 at 486.455 kb on - strand, within BBR_RS12210 at 486.455 kb on - strand, within BBR_RS12210 at 486.457 kb on + strand, within BBR_RS12210 at 486.468 kb on + strand, within BBR_RS12210 at 486.497 kb on - strand, within BBR_RS12210 at 486.538 kb on - strand, within BBR_RS12210 at 486.545 kb on + strand, within BBR_RS12210 at 486.546 kb on + strand, within BBR_RS12210 at 486.568 kb on - strand, within BBR_RS12210 at 486.581 kb on + strand, within BBR_RS12210 at 486.602 kb on - strand, within BBR_RS12210 at 486.618 kb on + strand, within BBR_RS12210 at 486.637 kb on - strand, within BBR_RS12210 at 486.679 kb on + strand at 486.691 kb on + strand at 486.738 kb on + strand at 486.780 kb on + strand at 486.788 kb on - strand at 486.831 kb on + strand at 486.841 kb on - strand at 486.859 kb on + strand at 486.862 kb on - strand at 486.898 kb on - strand at 486.900 kb on + strand at 486.908 kb on - strand at 486.921 kb on + strand at 486.929 kb on - strand at 486.947 kb on + strand at 486.952 kb on + strand at 486.955 kb on - strand at 486.955 kb on - strand at 486.974 kb on + strand at 486.982 kb on - strand at 486.991 kb on + strand at 486.991 kb on + strand at 486.999 kb on - strand at 486.999 kb on - strand at 486.999 kb on - strand at 486.999 kb on - strand at 487.046 kb on - strand at 487.050 kb on + strand at 487.077 kb on + strand at 487.087 kb on + strand at 487.102 kb on - strand at 487.111 kb on + strand at 487.111 kb on + strand at 487.119 kb on - strand at 487.125 kb on - strand at 487.137 kb on - strand at 487.171 kb on - strand at 487.171 kb on - strand at 487.178 kb on + strand at 487.186 kb on - strand at 487.186 kb on - strand at 487.186 kb on - strand at 487.203 kb on + strand at 487.203 kb on + strand at 487.211 kb on - strand at 487.211 kb on - strand at 487.269 kb on + strand at 487.324 kb on + strand at 487.332 kb on - strand at 487.332 kb on - strand at 487.352 kb on - strand at 487.371 kb on + strand at 487.371 kb on + strand at 487.375 kb on - strand at 487.386 kb on - strand at 487.415 kb on + strand at 487.419 kb on - strand at 487.441 kb on + strand at 487.450 kb on + strand at 487.450 kb on + strand at 487.455 kb on + strand at 487.519 kb on - strand at 487.529 kb on - strand at 487.547 kb on - strand at 487.550 kb on - strand at 487.628 kb on - strand at 487.640 kb on + strand at 487.648 kb on - strand at 487.648 kb on - strand at 487.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Isoleucine remove 485,185 + BBR_RS12200 0.29 -0.2 485,185 + BBR_RS12200 0.29 -0.9 485,185 + BBR_RS12200 0.29 +0.2 485,185 + BBR_RS12200 0.29 -3.1 485,187 - BBR_RS12200 0.30 -1.2 485,193 - BBR_RS12200 0.30 -0.8 485,200 - BBR_RS12200 0.31 -0.6 485,200 - BBR_RS12200 0.31 +0.9 485,206 + BBR_RS12200 0.32 +0.9 485,214 - BBR_RS12200 0.33 +0.1 485,219 + BBR_RS12200 0.34 +0.5 485,281 + BBR_RS12200 0.43 -2.4 485,281 + BBR_RS12200 0.43 +0.2 485,289 - BBR_RS12200 0.44 -0.1 485,289 - BBR_RS12200 0.44 +0.2 485,289 - BBR_RS12200 0.44 -0.3 485,291 + BBR_RS12200 0.44 -1.8 485,291 + BBR_RS12200 0.44 -1.8 485,296 - BBR_RS12200 0.45 +0.4 485,299 - BBR_RS12200 0.46 +0.1 485,304 - BBR_RS12200 0.46 +0.1 485,318 + BBR_RS12200 0.48 -1.1 485,318 + BBR_RS12200 0.48 -1.1 485,364 + BBR_RS12200 0.55 -0.3 485,364 + BBR_RS12200 0.55 -1.6 485,372 - BBR_RS12200 0.56 -1.9 485,372 - BBR_RS12200 0.56 -0.2 485,372 - BBR_RS12200 0.56 -0.1 485,377 + BBR_RS12200 0.57 -1.2 485,404 - BBR_RS12200 0.61 +0.3 485,404 - BBR_RS12200 0.61 +0.3 485,404 - BBR_RS12200 0.61 -0.4 485,456 + BBR_RS12200 0.68 -0.8 485,456 + BBR_RS12200 0.68 -1.8 485,464 - BBR_RS12200 0.69 +1.0 485,464 - BBR_RS12200 0.69 -1.2 485,475 - BBR_RS12200 0.71 +1.4 485,495 - BBR_RS12200 0.74 +0.1 485,512 - BBR_RS12200 0.76 +0.3 485,512 - BBR_RS12200 0.76 -2.0 485,512 - BBR_RS12200 0.76 -1.8 485,565 - BBR_RS12200 0.84 -0.2 485,565 - BBR_RS12200 0.84 -0.0 485,589 - BBR_RS12200 0.87 -0.3 485,618 + +0.8 485,623 + -2.0 485,623 + -1.2 485,623 + -2.1 485,623 + -0.4 485,630 + -1.2 485,636 - +0.3 485,636 - +0.4 485,655 - +0.8 485,673 + -1.0 485,682 - +0.1 485,693 - +0.4 485,712 + -2.3 485,712 + -1.4 485,722 + -1.2 485,722 + -1.2 485,725 - -0.0 485,730 - -1.0 485,730 - +0.1 485,739 + -1.6 485,747 - -1.3 485,750 + -1.9 485,752 + -3.4 485,758 - -0.5 485,758 - -1.0 485,758 - -0.4 485,758 - +0.0 485,760 - +0.3 485,766 - -1.6 485,799 - +0.9 485,961 + +0.6 485,970 + -0.3 485,970 + -0.9 485,989 - -0.1 486,005 - -1.2 486,085 - +0.9 486,119 + -1.8 486,134 - -1.8 486,152 + -1.4 486,193 + -2.5 486,275 + BBR_RS12210 0.20 -2.9 486,313 + BBR_RS12210 0.27 -1.6 486,318 + BBR_RS12210 0.28 -0.4 486,371 + BBR_RS12210 0.38 -1.5 486,381 + BBR_RS12210 0.40 -0.8 486,381 + BBR_RS12210 0.40 -0.9 486,381 + BBR_RS12210 0.40 -0.9 486,389 - BBR_RS12210 0.42 -1.3 486,389 - BBR_RS12210 0.42 -2.9 486,389 - BBR_RS12210 0.42 -0.4 486,447 + BBR_RS12210 0.53 -0.2 486,455 - BBR_RS12210 0.54 -1.5 486,455 - BBR_RS12210 0.54 -2.5 486,457 + BBR_RS12210 0.55 -1.5 486,468 + BBR_RS12210 0.57 +0.0 486,497 - BBR_RS12210 0.62 -3.0 486,538 - BBR_RS12210 0.70 -2.6 486,545 + BBR_RS12210 0.71 -1.6 486,546 + BBR_RS12210 0.71 -0.7 486,568 - BBR_RS12210 0.76 -3.4 486,581 + BBR_RS12210 0.78 -0.9 486,602 - BBR_RS12210 0.82 -1.3 486,618 + BBR_RS12210 0.85 -0.8 486,637 - BBR_RS12210 0.89 -2.0 486,679 + -1.1 486,691 + +0.4 486,738 + -2.1 486,780 + -0.9 486,788 - -2.1 486,831 + -0.9 486,841 - -0.8 486,859 + -1.1 486,862 - -1.0 486,898 - -1.9 486,900 + -1.3 486,908 - -0.5 486,921 + -2.7 486,929 - -2.4 486,947 + -0.9 486,952 + -0.3 486,955 - -1.4 486,955 - -2.4 486,974 + +0.1 486,982 - -1.1 486,991 + -2.1 486,991 + -2.0 486,999 - -0.9 486,999 - -1.8 486,999 - -0.6 486,999 - -2.1 487,046 - -1.3 487,050 + -0.5 487,077 + -0.9 487,087 + -1.1 487,102 - +1.4 487,111 + -0.1 487,111 + -0.9 487,119 - -1.0 487,125 - -1.2 487,137 - -2.6 487,171 - -1.2 487,171 - -1.9 487,178 + -1.7 487,186 - -2.1 487,186 - +0.1 487,186 - -0.8 487,203 + -0.7 487,203 + +0.4 487,211 - -0.1 487,211 - -0.4 487,269 + +1.2 487,324 + -0.3 487,332 - +0.2 487,332 - -0.4 487,352 - -2.7 487,371 + -1.7 487,371 + -0.6 487,375 - -1.0 487,386 - -2.2 487,415 + -1.6 487,419 - +0.2 487,441 + -1.4 487,450 + -1.1 487,450 + -0.6 487,455 + -2.2 487,519 - -0.9 487,529 - -0.4 487,547 - -0.6 487,550 - +0.2 487,628 - -0.7 487,640 + -1.9 487,648 - -0.8 487,648 - -0.6 487,683 + -1.4
Or see this region's nucleotide sequence