Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gmk and rpoZ are separated by 58 nucleotides rpoZ and Shewana3_3812 are separated by 43 nucleotides Shewana3_3812 and Shewana3_3813 are separated by 22 nucleotides Shewana3_3813 and Shewana3_3814 are separated by 232 nucleotides
Shewana3_3810: gmk - guanylate kinase (RefSeq), at 4,576,589 to 4,577,212
gmk
Shewana3_3811: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 4,577,271 to 4,577,549
rpoZ
Shewana3_3812: Shewana3_3812 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,577,593 to 4,579,695
_3812
Shewana3_3813: Shewana3_3813 - putative endoribonuclease L-PSP (RefSeq), at 4,579,718 to 4,580,101
_3813
Shewana3_3814: Shewana3_3814 - AMP-dependent synthetase and ligase (RefSeq), at 4,580,334 to 4,581,998
_3814
Position (kb)
4577
4578
4579
4580 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4577.162 kb on + strand at 4577.286 kb on + strand at 4577.369 kb on + strand, within rpoZ at 4577.474 kb on + strand, within rpoZ at 4577.558 kb on - strand at 4577.564 kb on + strand at 4579.265 kb on - strand, within Shewana3_3812 at 4579.437 kb on + strand, within Shewana3_3812 at 4579.502 kb on - strand at 4579.592 kb on + strand at 4579.836 kb on + strand, within Shewana3_3813 at 4579.836 kb on + strand, within Shewana3_3813 at 4580.001 kb on + strand, within Shewana3_3813 at 4580.017 kb on + strand, within Shewana3_3813 at 4580.017 kb on + strand, within Shewana3_3813 at 4580.025 kb on - strand, within Shewana3_3813 at 4580.025 kb on - strand, within Shewana3_3813 at 4580.025 kb on - strand, within Shewana3_3813 at 4580.056 kb on + strand, within Shewana3_3813 at 4580.064 kb on - strand at 4580.166 kb on + strand at 4580.166 kb on + strand at 4580.174 kb on - strand at 4580.184 kb on + strand at 4580.402 kb on - strand at 4580.500 kb on + strand at 4580.508 kb on - strand, within Shewana3_3814 at 4580.508 kb on - strand, within Shewana3_3814 at 4580.552 kb on + strand, within Shewana3_3814 at 4580.573 kb on + strand, within Shewana3_3814
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 4,577,162 + -3.0 4,577,286 + -0.1 4,577,369 + rpoZ Shewana3_3811 0.35 -2.6 4,577,474 + rpoZ Shewana3_3811 0.73 +1.1 4,577,558 - -0.1 4,577,564 + +1.3 4,579,265 - Shewana3_3812 0.80 +1.7 4,579,437 + Shewana3_3812 0.88 +1.5 4,579,502 - +2.1 4,579,592 + +1.6 4,579,836 + Shewana3_3813 0.31 +0.1 4,579,836 + Shewana3_3813 0.31 -1.2 4,580,001 + Shewana3_3813 0.74 -0.6 4,580,017 + Shewana3_3813 0.78 +0.2 4,580,017 + Shewana3_3813 0.78 -1.0 4,580,025 - Shewana3_3813 0.80 +0.1 4,580,025 - Shewana3_3813 0.80 +1.2 4,580,025 - Shewana3_3813 0.80 -0.1 4,580,056 + Shewana3_3813 0.88 -1.8 4,580,064 - -0.4 4,580,166 + -0.6 4,580,166 + +0.9 4,580,174 - -0.7 4,580,184 + +1.0 4,580,402 - -0.8 4,580,500 + +0.2 4,580,508 - Shewana3_3814 0.10 -1.8 4,580,508 - Shewana3_3814 0.10 +0.9 4,580,552 + Shewana3_3814 0.13 -0.7 4,580,573 + Shewana3_3814 0.14 -0.4
Or see this region's nucleotide sequence