Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2084 and Shew_2085 are separated by 2 nucleotides Shew_2085 and Shew_2086 overlap by 38 nucleotides Shew_2086 and Shew_2087 overlap by 26 nucleotides
Shew_2084: Shew_2084 - gonadoliberin III-related protein (RefSeq), at 2,410,221 to 2,411,726
_2084
Shew_2085: Shew_2085 - alpha-L-glutamate ligase-like protein (RefSeq), at 2,411,729 to 2,412,685
_2085
Shew_2086: Shew_2086 - hypothetical protein (RefSeq), at 2,412,648 to 2,414,423
_2086
Shew_2087: Shew_2087 - hypothetical protein (RefSeq), at 2,414,398 to 2,415,549
_2087
Position (kb)
2412
2413
2414
2415 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2412.259 kb on + strand, within Shew_2085 at 2412.420 kb on + strand, within Shew_2085 at 2414.038 kb on - strand, within Shew_2086 at 2414.162 kb on - strand, within Shew_2086 at 2414.281 kb on - strand at 2414.332 kb on - strand at 2415.041 kb on - strand, within Shew_2087 at 2415.351 kb on - strand, within Shew_2087 at 2415.385 kb on + strand, within Shew_2087
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,412,259 + Shew_2085 0.55 -0.2 2,412,420 + Shew_2085 0.72 +0.0 2,414,038 - Shew_2086 0.78 +2.8 2,414,162 - Shew_2086 0.85 +2.3 2,414,281 - +2.0 2,414,332 - +3.0 2,415,041 - Shew_2087 0.56 +2.5 2,415,351 - Shew_2087 0.83 +2.4 2,415,385 + Shew_2087 0.86 +2.2
Or see this region's nucleotide sequence