Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1760 and Shew_1761 are separated by 25 nucleotides Shew_1761 and Shew_1762 overlap by 20 nucleotides Shew_1762 and Shew_1763 are separated by 11 nucleotides
Shew_1760: Shew_1760 - (NiFe) hydrogenase maturation protein HypF (RefSeq), at 2,010,302 to 2,012,647
_1760
Shew_1761: Shew_1761 - hypothetical protein (RefSeq), at 2,012,673 to 2,014,460
_1761
Shew_1762: Shew_1762 - hydrogenase expression/formation protein (RefSeq), at 2,014,441 to 2,015,004
_1762
Shew_1763: Shew_1763 - Ni/Fe-hydrogenase, b-type cytochrome subunit (RefSeq), at 2,015,016 to 2,015,687
_1763
Position (kb)
2012
2013
2014
2015 Strain fitness (log2 ratio)
-2
-1
0
1 at 2012.063 kb on - strand, within Shew_1760 at 2012.212 kb on - strand, within Shew_1760 at 2012.950 kb on - strand, within Shew_1761 at 2013.313 kb on + strand, within Shew_1761 at 2013.389 kb on - strand, within Shew_1761 at 2013.414 kb on + strand, within Shew_1761 at 2013.843 kb on - strand, within Shew_1761 at 2013.919 kb on - strand, within Shew_1761 at 2013.965 kb on - strand, within Shew_1761 at 2013.980 kb on + strand, within Shew_1761 at 2014.472 kb on - strand at 2014.705 kb on + strand, within Shew_1762 at 2014.787 kb on - strand, within Shew_1762 at 2015.416 kb on - strand, within Shew_1763 at 2015.416 kb on - strand, within Shew_1763 at 2015.423 kb on + strand, within Shew_1763
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,012,063 - Shew_1760 0.75 -2.3 2,012,212 - Shew_1760 0.81 -0.0 2,012,950 - Shew_1761 0.15 -0.6 2,013,313 + Shew_1761 0.36 +0.2 2,013,389 - Shew_1761 0.40 -0.7 2,013,414 + Shew_1761 0.41 +0.1 2,013,843 - Shew_1761 0.65 -0.3 2,013,919 - Shew_1761 0.70 -0.2 2,013,965 - Shew_1761 0.72 +0.1 2,013,980 + Shew_1761 0.73 -0.6 2,014,472 - -0.3 2,014,705 + Shew_1762 0.47 +0.0 2,014,787 - Shew_1762 0.61 -1.4 2,015,416 - Shew_1763 0.60 +0.1 2,015,416 - Shew_1763 0.60 -0.1 2,015,423 + Shew_1763 0.61 +0.2
Or see this region's nucleotide sequence