Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and Shew_1658 overlap by 1 nucleotides Shew_1658 and Shew_1659 are separated by 85 nucleotides Shew_1659 and Shew_1660 are separated by 220 nucleotides Shew_1660 and fur are separated by 90 nucleotides
Shew_1657: sucC - succinyl-CoA synthetase subunit beta (RefSeq), at 1,906,416 to 1,907,582
sucC
Shew_1658: Shew_1658 - succinyl-CoA synthetase, alpha subunit (RefSeq), at 1,907,582 to 1,908,454
_1658
Shew_1659: Shew_1659 - ferredoxin-dependent glutamate synthase (RefSeq), at 1,908,540 to 1,910,213
_1659
Shew_1660: Shew_1660 - GCN5-related N-acetyltransferase (RefSeq), at 1,910,434 to 1,910,934
_1660
Shew_1661: fur - ferric uptake regulator (RefSeq), at 1,911,025 to 1,911,456
fur
Position (kb) 1908
1909
1910
1911 Strain fitness (log2 ratio) -2
-1
0
1 at 1909.085 kb on + strand, within Shew_1659 at 1909.585 kb on - strand, within Shew_1659 at 1909.792 kb on + strand, within Shew_1659 at 1910.042 kb on + strand, within Shew_1659 at 1910.097 kb on - strand at 1910.107 kb on - strand at 1910.640 kb on + strand, within Shew_1660 at 1910.926 kb on + strand at 1911.080 kb on - strand, within fur
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 1,909,085 + Shew_1659 0.33 +0.4 1,909,585 - Shew_1659 0.62 +1.3 1,909,792 + Shew_1659 0.75 +0.5 1,910,042 + Shew_1659 0.90 -0.2 1,910,097 - +0.3 1,910,107 - -2.3 1,910,640 + Shew_1660 0.41 +0.8 1,910,926 + +0.2 1,911,080 - fur Shew_1661 0.13 +0.8
Or see this region's nucleotide sequence